A simple and sensitive method for determining reducing sugars in plant tissues. Application to quantify the sugar content in quinoa (Chenopodium quinoa Willd.) seedlings

Prado, Fernando Eduardo; González, Juan Antonio Suarez; Boero, C.; Sampietro, Antonio Rodolfo

doi:10.1002/(sici)1099-1565(199803/04)9:2<58::aid-pca387>3.3.co;2-q

Cited by 14 publications

(15 citation statements)

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“…These tubes were placed on dry ice and transported back to the laboratory. Sugars were extracted from leaf tissues as follows (Prado et al 1998): a 500 mg fresh mass (FW) of each sample was homogenized with 2 cm 3 of 80% ethanol solution using a pestle and mortar. Following heating of the homogenate in a water bath at 75 °C for 10 min, the insoluble material was separated by centrifugation at 5000 × g for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Low‐temperature resistance in Polylepis tarapacana, a tree growing at the highest altitudes in the world

Rada

García-Núñez

Boero

et al. 2001

Plant Cell & Environment

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The Polylepis tarapacana forests found in Bolivia are unique with respect to their altitudinal distribution (4200-5200 m). Given the extreme environmental conditions that characterize these altitudes, this species has to rely on distinct mechanisms to survive stressful temperatures. The purpose of this study was to determine lowtemperature resistance mechanisms in P. tarapacana. Tissue was sampled for carbohydrate and proline contents and micro-climatic measurements were made at two altitudes, 4300 and 4850 m, during both the dry cold and wet warm seasons. Supercooling capacity (-3 to -6°C for the cold dry and -7 to -9°C for the wet warm season) and injury temperatures (-18 to -23°C for both seasons), determined in the laboratory, indicate that P. tarapacana is a frost-tolerant species. On the other hand, an increase in supercooling capacity, as the result of significant increase in total soluble sugar and proline contents, occurs during the wet warm season as a consequence of higher metabolic activity. Hence, P. tarapacana, a frost-tolerant species during the colder unfavourable season, is able to avoid freezing during the more favourable season when minimum night-time temperatures are not as extreme.

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Section: Methodsmentioning

confidence: 99%

Low‐temperature resistance in Polylepis tarapacana, a tree growing at the highest altitudes in the world

Rada

García-Núñez

Boero

et al. 2001

Plant Cell & Environment

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“…Sugar extraction: Flour (1 g) or fresh fruits (1 g) was extracted with ethanol 80% (4 mL) at 75˚C during 10 min and then centrifuged at 9000 × g during 5 min [13]. The remaining solids were extracted exhaustively with the same solvent system.…”

Section: Sugarmentioning

confidence: 99%

Nutritional, Antioxidant and Anti-Inflammatory Properties of <i>Cyclanthera pedata</i>, an Andinean Fruit and Products Derived from Them

Rivas¹,

Vignale²,

Ordóñez³

et al. 2013

FNS

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There is an increasing interest in food plant with health-giving effects. The aim of this research was to evaluate the phytochemical and nutraceutical profiles of Cyclanthera pedata fruits, a native fruit of the Andean region used by Incas communities called achojcha. Soluble protein (SP), total sugar (TS), free phenolic compounds (FPC), ascorbic acid (AA), total monomeric anthocyanin (TMA), condensed and hydrolizable tannin (CT, HT) were evaluated by sprectrophotometric methods in fresh fruits with and without heating and in flour obtained from fruits. Multielemental composition by ICP-MS was done. Antioxidant activity (AOA) was determined by ABTS and Linoleic acid--carotene methods. The inhibitory capacity of LOX, a pro-inflammatory enzyme was also analyzed. The achojcha fresh fruits and achojcha flour showed low amount calories. The flour was high in potassium (7400 mg·K/100 g) and low in sodium (77 mg Na/100 g). High levels of FPC (670 mg GAE/100 g) were found together with elevated levels of AA (123 mg AA/ 100 g). The TMA (0.6 mg C3-GE/100 g) as well as HT (3.4 mg PB2E/100 g) were also detected in flour. All preparations obtained with fresh and dried fruits showed AOA with SC 50 values between 1.8 to 14.5 μg GAE/mL. Inhibitory capacity on LOX was also demonstrated (IC 50 values of 40 µg GAE/mL). The fresh fruits and flour showed nutraceutical characteristics that are demanded by functional food and could be used as dietary supplement.

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“…Free sugars were extracted by sonicating ground fine roots in 80% ethanol (3 × 2 mL, 25 mg tissue). Starch was enzymatically converted to glucose prior to the colorimetric assay (Prado et al 1998).…”

Section: Root Chemistrymentioning

confidence: 99%

Altered growth and fine root chemistry of Betula papyrifera and Acer saccharum under elevated CO₂

Parsons¹,

Kopper²,

Lindroth³

2003

Can. J. For. Res.

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We investigated the effects of CO2 enrichment on fine root chemical composition of two tree species common to northern hardwood forests. Two-year-old Betula papyrifera and 3-year-old Acer saccharum saplings were grown under ambient (400 µmol·mol1) and elevated (700 µmol·mol1) CO2 in a glasshouse experiment. In both species, root/shoot ratios and fine root percentages (of total biomass) were unaltered by CO2 enrichment. Tissue nitrogen concentrations decreased in the fine roots, and consequently, C/N ratios increased with elevated CO2. In birch, only condensed tannins increased with CO2 enrichment, while root starch levels were conserved. In maple, neither condensed tannins nor hydrolysable tannins were positively influenced by elevated CO2. Both fine root biomass and chemistry responses of the tree saplings may be related to their successional status.

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A simple and sensitive method for determining reducing sugars in plant tissues. Application to quantify the sugar content in quinoa (Chenopodium quinoa Willd.) seedlings

Cited by 14 publications

References 0 publications

Low‐temperature resistance in Polylepis tarapacana, a tree growing at the highest altitudes in the world

Low‐temperature resistance in Polylepis tarapacana, a tree growing at the highest altitudes in the world

Nutritional, Antioxidant and Anti-Inflammatory Properties of <i>Cyclanthera pedata</i>, an Andinean Fruit and Products Derived from Them

Altered growth and fine root chemistry of Betula papyrifera and Acer saccharum under elevated CO₂

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A simple and sensitive method for determining reducing sugars in plant tissues. Application to quantify the sugar content in quinoa (Chenopodium quinoa Willd.) seedlings

Cited by 14 publications

References 0 publications

Low‐temperature resistance in Polylepis tarapacana, a tree growing at the highest altitudes in the world

Low‐temperature resistance in Polylepis tarapacana, a tree growing at the highest altitudes in the world

Nutritional, Antioxidant and Anti-Inflammatory Properties of &lt;i&gt;Cyclanthera pedata&lt;/i&gt;, an Andinean Fruit and Products Derived from Them

Altered growth and fine root chemistry of Betula papyrifera and Acer saccharum under elevated CO2

Contact Info

Product

Resources

About

Nutritional, Antioxidant and Anti-Inflammatory Properties of <i>Cyclanthera pedata</i>, an Andinean Fruit and Products Derived from Them

Altered growth and fine root chemistry of Betula papyrifera and Acer saccharum under elevated CO₂