A simple and sensitive method for the detection of Oxytetracycine levels in ready-to-eat beef by liquid chromatography-mass spectrometry

Mgonja, F.; Mosha, Resto D.; Mabiki, Faith; Choongo, Kennedy

doi:10.5897/ajpp2016.4553

Cited by 5 publications

(1 citation statement)

References 14 publications

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“…This poses a risk of microbial resistance in consumers due to the misuse of antibiotics during animal production. The LC-MS method is considered a fast, sensitive and easy means to detect residues and nonresidues in meat samples [ 51 ]. In addition, sample pre-treatment and extraction are carried out using the combined liquid–liquid extraction (LLE) approach because it is effective for the analysis complex samples [ 69 ].…”

Section: Drug Residues In Foodmentioning

confidence: 99%

Recent Advances in the Determination of Veterinary Drug Residues in Food

Pratiwi,

Ramadhanti,

Amatulloh

et al. 2023

Foods

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The presence of drug residues in food products has become a growing concern because of the adverse health risks and regulatory implications. Drug residues in food refer to the presence of pharmaceutical compounds or their metabolites in products such as meat, fish, eggs, poultry and ready-to-eat foods, which are intended for human consumption. These residues can come from the use of drugs in the field of veterinary medicine, such as antibiotics, antiparasitic agents, growth promoters and other veterinary drugs given to livestock and aquaculture with the aim of providing them as prophylaxis, therapy and for promoting growth. Various analytical techniques are used for this purpose to control the maximum residue limit. Compliance with the maximum residue limit is very important for food manufacturers according to the Food and Drug Administration (FDA) or European Union (EU) regulations. Effective monitoring and control of drug residues in food requires continuous advances in analytical techniques. Few studies have been reviewed on sample extraction and preparation techniques as well as challenges and future directions for the determination of veterinary drug residues in food. This current review focuses on the overview of regulations, classifications and types of food, as well as the latest analytical methods that have been used in recent years (2020–2023) for the determination of drug residues in food so that appropriate methods and accurate results can be used. The results show that chromatography is still a widely used technique for the determination of drug residue in food. Other approaches have been developed including immunoassay, biosensors, electrophoresis and molecular-based methods. This review provides a new development method that has been used to control veterinary drug residue limit in food.

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Section: Drug Residues In Foodmentioning

confidence: 99%

Recent Advances in the Determination of Veterinary Drug Residues in Food

Pratiwi,

Ramadhanti,

Amatulloh

et al. 2023

Foods

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Green validated chromatographic methods for simultaneous determination of co-formulated oxytetracycline HCl and flunixin meglumine in the presence of their impurities in different matrices

et al. 2022

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Green analytical chemistry is an important area of chemical sciences. So, developing competent eco-friendly analytical tools is a big challenge. This study devotes two chromatographic techniques for the simultaneous determination of a binary formulated mixture of oxytetracycline HCl (OXY) and flunixin meglumine (FLU), and their impurities tetracycline (TRC) and 2-chloronicotinic acid (CNA), respectively. Primarily, a TLC densitometric method is proposed and validated using TLC plates sprayed with 5% EDTA of pH 9 as a stationary phase and (acetonitrile/ distilled water/ ethanol 7:2:1, by volume) as a developing system. Well-separated spots are detected at 267 nm where linear relations have been achieved at 0.05–2 μg/band and 0.01–2 μg/band for OXY and FLU, respectively. The second developed and validated method is HPLC which is fulfilled on Hypersil BDS column-C18. A mobile phase of distilled water with 0.1%TFA/ acetonitrile in a ratio of 82: 18, v/v firstly then 70: 30, v/v after 9 min is used in a sequential isocratic elution at 210 nm for FLU and 267 nm for OXY, TRC, and CNA. Sensitive and wide linearity ranges are achieved at 0.05–200 μg/mL for both drugs. The two methods are applied successfully in cattle meat and milk for cited drugs determination. According to ICH guidelines, a validation study has been accomplished for the proposed methods. Statistical comparison has been carried out with official and reported methods. Eventually, the greenness of both procedures is evaluated using Eco-Scale which gives eco-friendly results. Graphical abstract

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Spectrophotometric Methods for Simultaneous Determination of Oxytetracycline HCl and Flunixin Meglumine in Their Veterinary Pharmaceutical Formulation

Merey

Abd-Elmonem

Nazlawy

et al. 2017

Journal of Analytical Methods in Chemistry

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Four precise, accurate, selective, and sensitive UV-spectrophotometric methods were developed and validated for the simultaneous determination of a binary mixture of Oxytetracycline HCl (OXY) and Flunixin Meglumine (FLU). The first method, dual wavelength (DW), depends on measuring the difference in absorbance (ΔA 273.4–327 nm) for the determination of OXY where FLU is zero while FLU is determined at ΔA 251.7–275.7 nm. The second method, first-derivative spectrophotometric method (1D), depends on measuring the peak amplitude of the first derivative selectively at 377 and 266.7 nm for the determination of OXY and FLU, respectively. The third method, ratio difference method, depends on the difference in amplitudes of the ratio spectra at ΔP 286.5–324.8 nm and ΔP 249.6–286.3 nm for the determination of OXY and FLU, respectively. The fourth method, first derivative of ratio spectra method (1DD), depends on measuring the amplitude peak to peak of the first derivative of ratio spectra at 296.7 to 369 nm and 259.1 to 304.7 nm for the determination of OXY and FLU, respectively. Different factors affecting the applied spectrophotometric methods were studied. The proposed methods were validated according to ICH guidelines. Satisfactory results were obtained for determination of both drugs in laboratory prepared mixture and pharmaceutical dosage form. The developed methods are compared favourably with the official ones.

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A simple and sensitive method for the detection of Oxytetracycine levels in ready-to-eat beef by liquid chromatography-mass spectrometry

Cited by 5 publications

References 14 publications

Recent Advances in the Determination of Veterinary Drug Residues in Food

Recent Advances in the Determination of Veterinary Drug Residues in Food

Green validated chromatographic methods for simultaneous determination of co-formulated oxytetracycline HCl and flunixin meglumine in the presence of their impurities in different matrices

Spectrophotometric Methods for Simultaneous Determination of Oxytetracycline HCl and Flunixin Meglumine in Their Veterinary Pharmaceutical Formulation

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