A simple and sensitive colorimetric pH meter based on DNA conformational switch and gold nanoparticle aggregation

Chen, Cuie; Song, Guangtao; Ren, Jinsong; Qu, Xiaogang

doi:10.1039/b812660g

Cited by 73 publications

(51 citation statements)

References 29 publications

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“…The degree of Au NP aggregation is expected to be influenced by the PCR products which serve as the linker molecules for forming the Au NP agglomeration. More cycles of the PCR procedure will lead to more PCR amplification which provides more linker molecules, and will result in a larger red-shift of UV-visible spectrum [21]. As expected, the spectral shift increased when the cycle number N was increased from 5 to 20 in our experiments (Fig.…”

Section: Resultssupporting

confidence: 64%

One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection

Cai

Zhang

et al. 2010

Nano Res.

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We report a novel method for rapid, colorimetric detection of a specific deoxyribonucleic acid (DNA) sequence by carrying out a polymerase chain reaction in the presence of gold nanoparticles functionalized with two primers. Extension of the primers when the target DNA is present as a template during the polymerase chain reaction process affords the complementary sequences on the gold nanoparticle surfaces and results in the formation of gold nanoparticle aggregates with a concomitant color change from red to pinkish/purple. This method provides a convenient and straightforward solution for ultrasensitive DNA detection without any further post-treatment of the polymerase chain reaction products being necessary, and is a promising tool for rapid disease diagnostics and gene sequencing.

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Section: Resultssupporting

confidence: 64%

One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection

Cai

Zhang

et al. 2010

Nano Res.

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“…The presence of NaCl could induce the formation of AuCl 4 À ions, which would lead to slow reaction kinetics and the subsequent formation of uniform shell structures (Fig. [35][36][37] The strong interactions between the adenine base and the gold surface are predominant in acidic pH conditions. S3, ESI †).…”

Section: Resultsmentioning

confidence: 99%

DNA-mediated control of Au shell nanostructure and controlled intra-nanogap for a highly sensitive and broad plasmonic response range

et al. 2015

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We report DNA-mediated simple synthetic methods to obtain anisotropic plasmonic nanostructures with a tailorable intra-nanogap distance ranging from 0.9 to 4.0 nm. Anisotropic half-shell structures with sub-1.0 nm intra-nanogaps showed a wavelength-independent surface-enhanced Raman scattering (SERS) intensity and a highly sensitive SERS response to NIR light. We found that the reaction conditions such as pH and NaCl concentration are responsible for the resulting shell structures and intra-nanogap distances. Three noticeable plasmonic nanostructures [i.e., half-shell with sub-1.0 nm nanogaps, closedshell with a wide nanogap (2.1 nm) and star-shaped with an irregular nanogap (1.5-4.0 nm)] were synthesized, and solution-based and single particle-based Raman measurements showed a strong relationship between the plasmonic structures and the SERS intensity. An understanding of DNA-mediated control for nanogap-engineered plasmonic nanostructures and studies of SERS-activity relationships using single particle-correlated measurements can provide new insights into the design of new plasmonic nanostructures and SERS-based biosensing applications.

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“…477 Gold-functionalised nanoparticles are more widely used, and are useful as they undergo a colour change from red to purple on aggregation, as well as possessing surface plasmon properties. The former has been used to detect hybridisation, 478,479 to detect conformational switching in i-motif sequences, 480 for the detection of mismatches using the repair enzyme MutS, 481 and linear amplification of DNA using Bst DNA polymerase. 482 Gold-conjugated oligonucleotides have been used to prepare crystalline assemblies using the complementarity of nucleotides, which may be of use in nanodevices.…”

Section: Oligonucleotide-metal Conjugatesmentioning

confidence: 99%

Nucleotides and Nucleic Acids; Oligo- and Polynucleotides

Loakes

2010

Organophosphorus Chemistry

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A simple and sensitive colorimetric pH meter based on DNA conformational switch and gold nanoparticle aggregation

Abstract: A simple and rapid colorimetric pH meter has been developed based on the conformational switch of i-motif DNA and non-crosslinking AuNP aggregation, the average accuracy of the nano-meter was found to be +/-0.04 pH unit across the physiological operating range.

Cited by 73 publications

References 29 publications

One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection

One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection

DNA-mediated control of Au shell nanostructure and controlled intra-nanogap for a highly sensitive and broad plasmonic response range

Nucleotides and Nucleic Acids; Oligo- and Polynucleotides

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