A simple and sensitive assay for ascorbate using potassium ferricyanide as spectroscopic probe reagent

Zhang, Hua; Li, Jing; Wang, Kui; Du, Xingqiang; Li, Quanmin

doi:10.1016/j.ab.2009.01.039

Cited by 18 publications

(14 citation statements)

References 17 publications

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“…These methods are routinely used in Srinagarind Hospital, Faculty of Medicine, Khon Kaen University. The plasma was used to analyze vitamin C levels with using Zhang’s method [18]. …”

Section: Methodsmentioning

confidence: 99%

Cashew apple juice supplementation enhanced fat utilization during high-intensity exercise in trained and untrained men

Prasertsri

Roengrit

Kanpetta

et al. 2013

Journal of the International Society of Sports Nutrition

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BackgroundExercise training is known to increase fat utilization during exercise. Diets containing antioxidants and branch chain amino acids (BCAAs) are also reported to have potential effects on fat utilization. Cashew apple juice (CAJ) comprises many nutritional components including vitamin C and BCAAs. This study aimed to investigate the effect of CAJ supplementation on substrate utilization during high-intensity exercise in trained and untrained subjects.MethodsTen trained and ten untrained men were randomly supplemented with either placebo (PLA) or CAJ at 3.5 ml/kg body mass (BM) /day for 4 weeks with a 4-week washout between treatments in a randomized cross-over design. Before and after the 4-week supplementations all subjects performed cycling exercise at 85% of maximal oxygen consumption for 20 minutes. At rest, before, and immediately after the exercise, venous blood samples were taken to determine glucose, insulin and lipid concentrations. Expired air was collected during the 20 minutes of exercise to calculate substrate utilization.ResultsDuring the exercise in both trained and untrained groups, there were lower carbohydrate (CHO) and higher fat oxidation rates and contributions to total energy expenditure after the CAJ supplementation compared to the PLA supplementation (p<0.05). These values were greater in the trained group than the untrained group except CHO oxidation rates (p<0.05), which were not significantly different. Moreover, in both trained and untrained groups, resting plasma vitamin C concentrations were significantly higher after the CAJ supplementation compared to the PLA supplementation, without any change after the PLA supplementation. These values were greater in the trained group than the untrained group (p<0.05). There were no significant differences in glucose, insulin or lipid concentrations between the groups’ blood samples.ConclusionThe findings of this study suggest that CAJ supplementation enhanced fat oxidation during exercise may enhance endurance performance, but specific studies are needed to assess this possibility.

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“…These methods are routinely used in Srinagarind Hospital, Faculty of Medicine, Khon Kaen University. The plasma was used to analyze vitamin C levels with using Zhang’s method [18]. …”

Section: Methodsmentioning

confidence: 99%

Cashew apple juice supplementation enhanced fat utilization during high-intensity exercise in trained and untrained men

Prasertsri

Roengrit

Kanpetta

et al. 2013

Journal of the International Society of Sports Nutrition

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“…1). Ferrocyanide has previously been employed as a redox mediator in electro-organic transformations [1], as an electron shuttle for respirating living cells [2], in thermoelectrochemical devices [3], in the electroanalytical determination of ascorbate [4], nitrite [5], hydrogen sulphide [6,7] or (via electrochemiluminescence) in the determination of thiols [8]. Ferrocyanide has been employed also as heterogeneous or polymer-immobilised redox catalyst for sulphide detection [9] and for nitrite detection [10].…”

Section: −mentioning

confidence: 99%

The immobilisation and reactivity of Fe(CN)63−/4− in an intrinsically microporous polyamine (PIM-EA-TB)

Wang

Malpass‐Evans

Carta

et al. 2020

J Solid State Electrochem

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Protonation of the molecularly rigid polymer of intrinsic microporosity PIM-EA-TB can be coupled to immobilisation of Fe(CN) 6 3−/4− (as well as immobilisation of Prussian blue) into 1-2 nm diameter channels. The resulting films provide redoxactive coatings on glassy carbon electrodes. Uptake, transport, and retention of Fe(CN) 6 3−/4− in the microporous polymer are strongly pH dependent requiring protonation of the PIM-EA-TB (pK A ≈ 4). Both Fe(CN) 6 4− and Fe(CN) 6 3− can be immobilised, but Fe(CN) 6 4− appears to bind tighter to the polymer backbone presumably via bridging protons. Loss of Fe(CN) 6 3−/4− by leaching into the aqueous solution phase becomes significant only at pH > 9 and is likely to be associated with hydroxide anions directly entering the microporous structure to combine with protons. This and the interaction of Fe(CN) 6 3−/4− and protons within the molecularly rigid PIM-EA-TB host are suggested to be responsible for retention and relatively slow leaching processes. Electrocatalysis with immobilised Fe(CN) 6 3−/4− is demonstrated for the oxidation of ascorbic acid.

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“…Only a few studies, however, have been performed to use immobilized Fe(CN) 6 3− as an electron mediator to adjust the electron transfer process [9][10][11][12][13][14][15][16]. For example, Yang et al [9] fabricated a copper hexacyanoferrate (CuHCF) multilayer on the 4-aminobenzoic acid (4-ABA)-modified GCE based on the complexation of metal ion with ferricyanide.…”

Section: Introductionmentioning

confidence: 97%

Development of an electrochemical ascorbic acid sensor based on the incorporation of a ferricyanide mediator with a polyelectrolyte–calcium carbonate microsphere

Tang

Liu

et al. 2010

Electrochimica Acta

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A simple and sensitive assay for ascorbate using potassium ferricyanide as spectroscopic probe reagent

Cited by 18 publications

References 17 publications

Cashew apple juice supplementation enhanced fat utilization during high-intensity exercise in trained and untrained men

Cashew apple juice supplementation enhanced fat utilization during high-intensity exercise in trained and untrained men

The immobilisation and reactivity of Fe(CN)63−/4− in an intrinsically microporous polyamine (PIM-EA-TB)

Development of an electrochemical ascorbic acid sensor based on the incorporation of a ferricyanide mediator with a polyelectrolyte–calcium carbonate microsphere

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