A simple and robust in vitro assay to quantify the vigour of oilseed rape lines and hybrids

Block, Marc De; Brouwer, Dirk De

doi:10.1016/s0981-9428(02)01439-0

Cited by 13 publications

(20 citation statements)

References 35 publications

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“…A positive correlation between yield and low cellular respiration rates has been demonstrated in canola, Lolium perenne, tomato (Solanum esculentum), and cucumber (Cucumis sativus) (10)(11)(12)(13)(14)(15)(16). Based on the published experimental concept (2-4), an artificial bidirectional selection procedure was established in canola for high and low cellular respiration rates.…”

Section: Resultsmentioning

confidence: 99%

Energy use efficiency is characterized by an epigenetic component that can be directed through artificial selection to increase yield

Hauben

Haesendonckx

Standaert

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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174

140

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Quantitative traits, such as size and weight in animals and seed yield in plants, are distributed normally, even within a population of genetically identical individuals. For example, in plants, various factors, such as local soil quality, microclimate, and sowing depth, affect growth differences among individual plants of isogenic populations. Besides these physical factors, also epigenetic components contribute to differences in growth and yield. The network that regulates crop yield is still not well understood. Although this network is expected to have epigenetic elements, it is completely unclear whether it would be possible to shape the epigenome to increase crop yield. Here we show that energy use efficiency is an important factor in determining seed yield in canola (Brassica napus) and that it can be selected artificially through an epigenetic feature. From an isogenic canola population of which the individual plants and their self-fertilized progenies were recursively selected for respiration intensity, populations with distinct physiological and agronomical characteristics could be generated. These populations were found to be genetically identical, but epigenetically different. Furthermore, both the DNA methylation patterns as well as the agronomical and physiological characteristics of the selected lines were heritable. Hybrids derived from parent lines selected for high energy use efficiencies had a 5% yield increase on top of heterosis. Our results demonstrate that artificial selection allows the increase of the yield potential by selecting populations with particular epigenomic states. Brassica napus ͉ epigenome

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Section: Resultsmentioning

confidence: 99%

Energy use efficiency is characterized by an epigenetic component that can be directed through artificial selection to increase yield

Hauben

Haesendonckx

Standaert

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

174

140

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“…This enhanced energy-use efficiency could be rapidly assessed with an in vitro stress assay that allows us to monitor vigor. In this assay, total NAD(P)H and ATP contents and the capacity of plants to reduce 2,3,5-triphenyltetrazolium chloride after a few hours of high-light (HL) stress are quantified (9,21). To obtain a better understanding of the molecular mechanisms driving the stress tolerance in hpAtPARP2 plants, we followed the gene expres- sion dynamics in both wild-type and transgenic plants during this HL stress-dependent assay.…”

Section: Genome-wide Transcriptome Analysis Of Stress-tolerant Hpatparp2mentioning

confidence: 99%

Silencing of poly(ADP-ribose) polymerase in plants alters abiotic stress signal transduction

Vanderauwera

Block

Steene

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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154

118

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Transgenic plants with reduced poly(ADP-ribose) polymerase (PARP) levels have broad-spectrum stress-resistant phenotypes. Both Arabidopsis thaliana and oilseed rape (Brassica napus) lines overexpressing RNA interference-PARP constructs were more resistant to various abiotic stress treatments in laboratory and greenhouse experiments without negative effects on growth, development, and seed production. This outperforming stress tolerance was initially attributed solely to a maintained energy homeostasis due to reduced NAD ؉ consumption. We show that in PARP2-deficient Arabidopsis plants, the observed abiotic stress resistance can also be explained by alterations in abscisic acid levels that facilitate the induction of a wide set of defense-related genes.gene expression ͉ protection ͉ transcriptome analysis

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“…Diminished cell viability within seeds, as detected by TTC, occurred earlier than the loss of seed germination capacity (Figures and S1). Mitochondrial dehydrogenase reduces TTC, and the efficiency of formation of red formazan depends on the activity of cytochrome oxidase (Block and Brouwer, ). Transmission electron microscopy results confirmed that typical mitochondrial swelling was an early cellular alteration in cotyledons, after 2 days of CDT (Figure ).…”

Section: Discussionmentioning

confidence: 99%

Reactive oxygen species‐provoked mitochondria‐dependent cell death during ageing of elm (Ulmus pumila L.) seeds

Wang

Xue

et al. 2015

The Plant Journal

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These authors contributed equally to this work. SUMMARYPrevious studies have shown that controlled deterioration treatment (CDT) induces programmed cell death in elm (Ulmus pumila L.) seeds, which undergo certain fundamental processes that are comparable to apoptosis in animals. In this study, the essential characteristics of mitochondrial physiology in elm seeds during CDT were identified by cellular ultrastructural analysis, whole-body optical imaging, Western blotting and semi-quantitative RT-PCR. The alteration in mitochondrial morphology was an early event during CDT, as indicated by progressive dynamic mitochondrial changes and rupture of the mitochondrial outer membrane; loss of mitochondrial transmembrane potential (Dw m ) ensued, and mitochondrial ATP levels decreased. The mitochondrial permeability transition pore inhibitor cyclosporine A effectively suppressed these changes during ageing. The in situ localization of production of reactive oxygen species (ROS), and evaluation of the expression of voltage-dependent anion-selective channel and cyclophilin D indicated that the levels of mitochondrial permeability transition pore components were positively correlated with ROS production, leading to an imbalance of the cellular redox potential and ultimately to programmed cell death. Pre-incubation with ascorbic acid slowed loss of mitochondrial Dw m , and decreased the effect of CDT on seed viability. However, there were no significant changes in multiple antioxidant elements or chaperones in the mitochondria during early stages of ageing. Our results indicate that CDT induces dynamic changes in mitochondrial physiology via increased ROS production, ultimately resulting in an irreversible loss of seed viability.

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A simple and robust in vitro assay to quantify the vigour of oilseed rape lines and hybrids

Cited by 13 publications

References 35 publications

Energy use efficiency is characterized by an epigenetic component that can be directed through artificial selection to increase yield

Energy use efficiency is characterized by an epigenetic component that can be directed through artificial selection to increase yield

Silencing of poly(ADP-ribose) polymerase in plants alters abiotic stress signal transduction

Reactive oxygen species‐provoked mitochondria‐dependent cell death during ageing of elm (Ulmus pumila L.) seeds

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