A Simple and Reproducible Method for Collecting Nasal Secretions in Frail Elderly Adults, for Measurement of Virus‐Specific IgA

Walsh, Edward E.; Falsey, Ann R.

doi:10.1086/314726

Cited by 29 publications

(17 citation statements)

References 14 publications

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“…In RSV, serum neutralising antibody titres have been shown to negatively correlate with risk of medical attendance with RSV disease but no upper threshold of protection has been identifiable, with serum neutralising antibodies only broadly dividing individuals into those with minimal antibody (and therefore highly susceptible) and those with adult-like levels [39]. Previous observational studies indicated that local RSV-specific antibodies might be better correlates of protection but suggested that protective antibody levels were not well maintained [40,41]. To address these questions, we used the experimental human infection system to study the relationship between pre-existing local and systemic antibodies with infection [42].…”

Section: Experimental Challenge Provides Insights Into Pathogenesismentioning

confidence: 99%

Controlled human infection with RSV: The opportunities of experimental challenge

Habibi

Chiu

2017

Vaccine

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Section: Experimental Challenge Provides Insights Into Pathogenesismentioning

confidence: 99%

Controlled human infection with RSV: The opportunities of experimental challenge

Habibi

Chiu

2017

Vaccine

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“…The titer of nasal IgA to F, Ga, and Gb was determined using published methods (23). Total protein in each nasal sample was determined (Micro BCA, Rockford, IL) and nasal IgA was corrected to a total protein of 100 g/ml.…”

Section: Rsv Serologymentioning

confidence: 99%

Detection of Respiratory Syncytial Virus in Adults with Chronic Obstructive Pulmonary Disease

Falsey

Formica

Hennessey

et al. 2006

Am J Respir Crit Care Med

129

107

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Rationale: Recently, respiratory syncytial virus (RSV) RNA has been identified by reverse transcriptase-polymerase chain reaction (RT-PCR) from a high percentage of patients with stable chronic obstructive pulmonary disease (COPD). These data raise the possibility of persistent low-grade infection in this population, which could have implications in COPD pathogenesis. Objectives: RSV persistence was investigated by testing respiratory secretions from subjects with COPD during illness and at regular intervals over 1 yr. Methods: Nasal and sputum samples from subjects with COPD were tested by one-tube nested RT-PCR for RSV every 2 mo and during respiratory illnesses for 1 yr. Subjects positive for RSV were evaluated weekly until negative in two consecutive samples. Nasal secretions and serum were tested for RSV antibody. A rise of fourfold or greater was defined as evidence of RSV infection. Results: A total of 112 patients were enrolled and the illnesses of 92 patients were evaluated. RSV was detected by RT-PCR in 6/92 (6.5%) illness nasal samples versus 0/685 routine nasal samples and in 5/69 (7.2%) illness sputum samples versus 3 /315 (0.9%) routine. Four additional RSV infections were identified by serum antibody responses. Of the RSV infections 86% were associated with serum or nasal antibody responses and 73% had symptoms of acute respiratory illness. Conclusions: Most RSV infections in patients with COPD are associated with symptomatic respiratory illnesses and measurable immune responses. Our data do not support the concept of RSV persistence in this population. Keywords: chronic obstructive pulmonary disease exacerbation; persistent infection; viral infectionChronic obstructive pulmonary disease (COPD) is a group of disorders characterized by airflow obstruction that can be associated with breathing-related symptoms such as chronic cough, dyspnea, and wheezing (1). It is estimated that approximately 24 million adults in the United States have impaired lung function due to COPD. During 2000, COPD was responsible for 8 million physician office visits, 1.5 million emergency room visits, 726,000 hospitalizations, and 119,000 deaths. Recurrent acute exacerbations of COPD contribute substantially to the morbidity and mortality of this condition and may be due to infections with bacteria, viruses, or both (2-4). In studies of COPD exacerbation, respiratory syncytial virus (RSV) has been identified with variable frequency ranging from 0.8 to 22% depending on the diagnostic methods used (5-16). Recently investigators from the United Kingdom and Germany identified RSV RNA by reverse In our laboratory, we have developed a sensitive and specific real-time, one-tube nested RT-PCR for the diagnosis of RSV in adults (19,20). Because the assay is performed without opening the reaction tube, PCR contamination has been markedly reduced while the sensitivity of a nested assay is retained. Therefore, we sought to explore the question of RSV persistence in patients with COPD by testing upper-and lower-respiratorytract secreti...

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“…During enrollment, before the RSV season, demographic and medical information was recorded, and a blood and nasal swab sample was obtained for the determination of antibodies. The nasal-swab sample for antibody was obtained by rotating a moistened cottontipped swab for 5 s in the anterior nares, at the level of the turbinates, and eluted into 3 mL of sterile saline [10]. Samples were frozen at Ϫ80ЊC until analysis.…”

Section: Study Subjects and Illness Surveillancementioning

confidence: 99%

Humoral and Mucosal Immunity in Protection from Natural Respiratory Syncytial Virus Infection in Adults

2004

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Virus-specific nasal immunoglobulin (Ig) A and serum antibody titers in 67 respiratory syncytial virus (RSV)-infected adults were compared with titers in age-matched uninfected control subjects. Control subjects had significantly higher levels of nasal IgA and serum IgG to the RSV F, Ga, and Gb proteins than infected subjects. Serum neutralizing-antibody titers to group A and B RSV strains were also lower in infected subjects, although differences were not as large. Multivariate analysis found that low RSV-specific nasal IgA was an independently significant risk factor for RSV infection.

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A Simple and Reproducible Method for Collecting Nasal Secretions in Frail Elderly Adults, for Measurement of Virus‐Specific IgA

Cited by 29 publications

References 14 publications

Controlled human infection with RSV: The opportunities of experimental challenge

Controlled human infection with RSV: The opportunities of experimental challenge

Detection of Respiratory Syncytial Virus in Adults with Chronic Obstructive Pulmonary Disease

Humoral and Mucosal Immunity in Protection from Natural Respiratory Syncytial Virus Infection in Adults

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