A Simple and Rapid Staining Technique for Plastic Embedded Cartilage and Bone

Grill, Vittorio; Zweyer, Marina; Bareggi, Renato; Martelli, Alberto M.; Basa, M; Narducci, Paola

doi:10.3109/10520299509108321

Cited by 5 publications

(4 citation statements)

References 15 publications

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“…The occurrence of perinuclear spheroids in chondrocytes (inclusion bodies in our terminology) has been noted by many investigators. In earlier references (Maximov and Bloom 1949), they were invariably referred to as vacuoles, and subsequently as clear vacuoles, stained material (Grill et al 1995), or as dense bodies (Bonucci and Silverstrini 1994). Regardless of the appellation, they evidently are extremely susceptible to vagaries of fixation and ancillary procedures (Bonucci and Silverstrini 1994), thus making their descriptive identification far from certain.…”

Section: Discussionmentioning

confidence: 95%

Transmission light microscopy of structurally colored semithin cartilage sections

Malinin

1997

Histochemistry and Cell Biology

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Conversion of osmified tracheal cartilage constituents into an array of laminar interference gratings has been attained by three tandem reactions. Oxidation of semithin, LR white-embedded cartilage sections by acetic anhydride in dimethyl sulfoxide is the first step in the conversion process. Subsequent addition reactions of oxidized cartilage pyranoses and furanoses with thiocarbohydrazide constitutes the second step. Reduction of silver proteinate by thiocarbohydrazones and the concomitant coating of cartilage constituents with silver gratings completes the conversion of cartilage sections into a system of layered interference filters. In transmitted light, all components of converted cartilage display vivid structural colors, which allow detailed microscopic analysis of structurally colored cellular and extracellular cartilage constituents.

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Section: Discussionmentioning

confidence: 95%

Transmission light microscopy of structurally colored semithin cartilage sections

Malinin

1997

Histochemistry and Cell Biology

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“…We chose to stain with haematoxylin only since this is one of the most used stains for envisioning overall tissue morphology. There are a number of relatively simple stainings that could enhance visibility of bone microstructure (Xipell et al, 1974;Bain et al, 1990;Gruber & Mekikian, 1991;Derkx & Birkenhager-Frenkel, 1995;Grill et al, 1995). Testing of these could present interesting results.…”

Section: Discussionmentioning

confidence: 99%

“…In clinical pathology, staining is used on a regular basis to diagnose or enhance tissue architecture. In those cases, tissue specimens are usually decalcified and cut by a microtome (Romeis, 1989) or embedded undecalcified and cut with a high-duty microtome (Wallin et al, 1985;Gruber & Mekikian, 1991;Grill et al, 1995). In those cases in which a grinding technique is used, the resin is removed with strong solutes prior to staining (Xipell et al, 1974;Romeis, 1989;Derkx & Birkenhager-Frenkel, 1995).…”

Section: Introductionmentioning

confidence: 99%

Manual for the Preparation and Staining of Embedded Natural Dry Bone Tissue Sections for Microscopy

Boer

Aarents

Maat

2011

Intl J of Osteoarchaeology

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In the last decade, the use of light microscopy has been firmly established for the investigation of exhumated human bone tissue. As a rule, these remains cannot be decalcified; thus, they are most commonly prepared for microscopic analysis as ground thin sections. These ground sections are of great value in diagnosing disease, in estimating age or in assessing taphonomic alteration. As bone is sometimes fragile and can be damaged by the grinding process, the specimen is occasionally supported by an embedding medium. In contrast to the vast amount of research conducted on embedded and unembedded unstained bone material, the use of histological stains on undecalcified dry bone tissue has been long neglected. In this article, a new method for embedding, sawing and grinding dry bone tissue is presented. The produced sections are subsequently stained with haematoxylin. The results show that even ground sections of fragile bone can be made in a quick and easy manner. Staining these sections enhances the envisioning of microarchitecture and taphonomical processes. In addition, the sections stay open for inspection under polarized light. The results were consistent throughout the used bone material. To keep the method as accessible and comprehensive as possible, a step-wise manual is provided. An additional troubleshooting paragraph discusses the most often encountered problems and provides solutions.

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“…There was also the possibility of histochemical staining, further extending the scope of the subsequent histological examination (De Boer et al, 2012). Other studies mention combinations of various techniques for embedding mineralised bone in resin with certain staining technologies (Gruber & Pertchoui, 1991;Grill et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Different Preparation Techniques - Similar Results? On the Quality of Thin-Ground Sections of Archaeological Bone

Haas

Storå

2014

Int. J. Osteoarchaeol.

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Palaeohistology as a valuable diagnostic instrument is dependent on the production of high‐quality thin‐ground sections from dry bone. The objective of this study was to consider technical differences and assess the qualitative outcomes of five techniques for preparing thin‐ground sections from dry archaeological bone. Established techniques with long follow‐up times and excellently documented results were compared with simpler and cheaper time‐saving techniques. Evaluations were made of the quality of thin sections obtained by one classical machine‐based embedding technique, two revised versions of the same technique, one manual moulding technique based on Frost's rapid technique and one manual hybrid technique. Five osteological specimens of differing quality were prepared following the manuals for these five techniques and examined microscopically with respect to a list of standardised histological and diagenetic parameters. Alterations in the specimens attributable to preparation effects were recorded, and observations were scored with reference to three criteria: section quality, technical quality and staining. The results show that embedding techniques are to prefer. Superglue should not be used as a mounting or embedding medium. Manual grinding comes with several limitations, and machine cutting and grinding are preferred. Haematoxylin staining can be successfully applied to embedded specimens, giving more information on microscopic diagenetic processes. A stepwise manual for a revision of the classical embedding technique is presented. The time required for producing sections using classical embedding techniques is shortened from 6 weeks to 3.7 days by refining the preparation/polymerization processes involved with no loss of osteological data. Copyright © 2014 John Wiley & Sons, Ltd.

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A Simple and Rapid Staining Technique for Plastic Embedded Cartilage and Bone

Cited by 5 publications

References 15 publications

Transmission light microscopy of structurally colored semithin cartilage sections

Transmission light microscopy of structurally colored semithin cartilage sections

Manual for the Preparation and Staining of Embedded Natural Dry Bone Tissue Sections for Microscopy

Different Preparation Techniques - Similar Results? On the Quality of Thin-Ground Sections of Archaeological Bone

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