A simple and rapid method of staining malarial parasites in thick blood smears

Field, J. W.

doi:10.1016/s0035-9203(40)90068-2

Cited by 11 publications

(3 citation statements)

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“…After incubation for one hour at 37°C the filter was removed from the chemotaxis apparatus, the top wiped to remove residual cells, and the filter then fixed in methanol for two minutes. Cells were stained using a modified Field's method (Field, 1940). The area of the lower surface of the filter occupied by cells was measured with an AMS 40-10 image analyser (Analytical Measuring Systems, Saffron Walden, Cambridgeshire), the filter being viewed through a 10 x objective, with a VG-9 green filter and ND 35% neutral density filter (Microscope Service and Sales, Egham, Surrey), placed in the incident light path (Harvath et al, 1980 tactic responses to ZAP (0.1-25.0%, n = 8), casein (0.1-2.OmgmlP', n = 5) and fMLP (10-°10-6M, n = 4), were determined.…”

Section: Cell Purity and Viabilitymentioning

confidence: 99%

Contrasting in vitro lymphocyte chemotactic activity of the hydroxyl enantiomers of 12‐hydroxy‐5,8,10,14‐eicosatetraenoic acid

Bacon

Camp

Cunningham

et al. 1988

British J Pharmacology

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The chemotactic activity of 12(R)‐hydroxy‐5,8,10,14‐eicosatetraenoic acid (12(R)‐HETE), 12(S)‐HETE and leukotriene B4 (LTB4) for human mixed peripheral blood lymphocytes has been assessed in a 48‐well microchemotaxis assay. Responses to the standard lymphocyte chemoattractants, zymosan‐activated plasma, casein and N‐formyl‐methionyl‐leucyl‐phenylalanine (fMLP) were also measured. 12(R)‐HETE was shown to be chemotactic for lymphocytes over the range 5 × 10−7 to 5 × 10−5 m. In contrast, negligible chemotactic responses to 12(S)‐HETE were obtained. LTB4 was 200 times more potent than 12(R)‐HETE as a lymphocyte chemoattractant, although maximal responses to the two agonists were similar. 12(R)‐HETE and LTB4, which are present in extracts of samples from the skin lesions of psoriasis, may be, at least in part, responsible for the lymphocyte infiltrate which is a characteristic feature of this disease.

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Section: Cell Purity and Viabilitymentioning

confidence: 99%

Contrasting in vitro lymphocyte chemotactic activity of the hydroxyl enantiomers of 12‐hydroxy‐5,8,10,14‐eicosatetraenoic acid

Bacon

Camp

Cunningham

et al. 1988

British J Pharmacology

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“…MF has been used widely on malaria (Field 1940;Fenton and Innes 1945;Reilly et al 1997), filaria (Sivanandam and Mak 1975), Leishmania (Chunge et al 1989), Acanthamoeba spp. (Pirehma et al 1999) and trypanosomes (Sindato et al 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Over the past many years, the conventional Trypan Blue has been used for assessing cell viability of various parasites and cells as well (Gold 1997;Wiehart et al 2006;Jin et al 2009;Tan et al 2010). MF has been used widely on malaria (Field 1940;Fenton and Innes 1945;Reilly et al 1997), filaria (Sivanandam and Mak 1975), Leishmania (Chunge et al 1989), Acanthamoeba spp. (Pirehma et al 1999) and trypanosomes (Sindato et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Modified Field stain - rapid viability test for Trichomonas vaginalis

Afzan

Sivanandam

2011

Journal of Applied Microbiology

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Aims: We previously reported that Modified Field Stain (MF) can be used as a rapid stain for diagnosis. In the present study we extend the observation to include the stain as an alternative method to assess viability of the cells. Methods and Results: Six isolates of Trichomonas vaginalis were used to assess the utility of the Modified Field stain as a rapid viability test for T. vaginalis and to compare with 0AE4% Trypan Blue dye exclusion test in three conditions; normal in vitro culture growth using Hollander medium, lysed in distilled water and treated with metronidazole. MF stain showed similar growth profile pattern as Trypan Blue dye exclusion for identifying viable cells of T. vaginalis. Although, Trypan Blue dye exclusion test is ready made, rapid and widely used in laboratory as reliable viability assay, however, the limitation using Trypan Blue is the dye was unable to show internal morphological changes during the parasite's transition from being viable to non-viable. On day 3 where cultures peaked the correlation factor of both assays done to assess the viability of parasites harvested from the controls, metronidazole and distilled water treated parasites were more than 0AE9 respectively. Conclusions: This confirms that MF staining does not only record permanently the morphological changes and retain internal structural details but also provides a reliable and rapid viability assay for the parasites. Significance and Impact of the Study: Therefore, in our study, Modified Field's stain may offer the researchers and laboratory technologists the opportunity to get the result on the same day and the most important thing is the ability to differentiate between viable and non-viable of T. vaginalis under three different conditions (normal culture, drug and distilled water condition). Modified Field's staining method enhanced the morphological identification of T. vaginalis compared to Trypan Blue dye exclusion.

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The rapid isolation from human blood of concentrated, white-cell-free preparations of Plasmodium falciparum

Williamson¹,

Cover²

1975

Transactions of the Royal Society of Tropical Medicine and Hygi

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The sucrose gradient centrifugation method has been applied to representative samples of human (Gambian) blood infected with ring, trophozoite, schizont and gametocyte stages of P. falciparum in order to assess quantitatively the efficiency of recovery, white cell removal and the degree of enrichment of the infected cell fraction. Maximal white cell removal was 90%. (a.v.) Average infected cell recoveries varied with the level of white cell contamination, namely 47% (2-5% WBC), 34% (1-2% WBC) and 24% (smaller than 1% WBC); infected cells were enriched 2-9-fold on average, and up to 22-fold in the case of gametocytes. Preliminary attempts to prepare free parasites by nitrogen cavitation of infected cells showed that disruption took place at much lower pressures than those required to break normal red cells. Gel diffusion analyses showed that the most highly purified infected cell preparations retained the full precipitinogenic spectrum of the original crude preparation.

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A simple and rapid method of staining malarial parasites in thick blood smears

Cited by 11 publications

References 0 publications

Contrasting in vitro lymphocyte chemotactic activity of the hydroxyl enantiomers of 12‐hydroxy‐5,8,10,14‐eicosatetraenoic acid

Contrasting in vitro lymphocyte chemotactic activity of the hydroxyl enantiomers of 12‐hydroxy‐5,8,10,14‐eicosatetraenoic acid

Modified Field stain - rapid viability test for Trichomonas vaginalis

The rapid isolation from human blood of concentrated, white-cell-free preparations of Plasmodium falciparum

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