A simple and rapid liquid chromatography method for simultaneous determination of zidovudine and nevirapine in plasma

Ramachandran, Geetha; Hemanthkumar, Agibothu K.; Kumaraswami, V.; Swaminathan, Soumya

doi:10.1016/j.jchromb.2006.06.014

Cited by 42 publications

(25 citation statements)

References 24 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A few chromatographic methods viz., HPLC, LC-MS or LC-MS/MS have been reported for the determination of NVP [11][12][13][14][15][16]. These methods require long analysis times, elaborate extraction and purification steps, or on-line sample extraction, and are relatively costly.…”

Section: Figure 1 Structure Of Nevirapinementioning

confidence: 99%

Electrochemical studies of nevirapine, an anti-HIV drug, and its assay in tablets and biological samples

Teradal¹,

Prashanth²,

Seetharamappa³

2012

J. Electrochem. Sci. Eng.

View full text Add to dashboard Cite

show abstract

Section: Figure 1 Structure Of Nevirapinementioning

confidence: 99%

Electrochemical studies of nevirapine, an anti-HIV drug, and its assay in tablets and biological samples

Teradal¹,

Prashanth²,

Seetharamappa³

2012

J. Electrochem. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…The HPLC methods described in the literature for determination of AZT levels in animal and human plasma (Molema et al, 1992;Schrive and Plasse, 1994;Aymard et al, 2000;Tan and Boudinot, 2000;Clark et al, 2001;Marchei et al, 2002;Ramachandran et al, 2006;Tarinas et al, 2007;Lewis et al, 2007;Lefebvre et al, 2007) present high analysis costs of because they use sample treatment processes based on solid phase extraction or several steps of liquid-liquid extraction. These assay methods are relatively time-consuming or use expensive instrumentation not readily accessible to many groups.…”

Section: Discussionmentioning

confidence: 99%

“…These methods involve ultraviolet assay (Baig et al, 2001;Erk, 2004), liquid chromatography/tandem mass spectrometry Pereira et al, 2000;Alnouti et al, 2005;Mudigonda et al, 2008), micellar capillary electrophoresis and high-performance liquid chromatography (HPLC) with UV detection (Molema et al, 1992;Schrive and Plasse, 1994;Aymard et al, 2000;Tan and Boudinot, 2000;Clark et al, 2001;Marchei et al, 2002;Ramachandran et al, 2006;Tarinas et al, 2007;Lewis et al, 2007;Lefebvre et al, 2007). Most of the methods using HPLC with UV detection methods described until now are relatively timeconsuming or they use expensive instrumentation not readily accessible to many groups.…”

Section: Introductionmentioning

confidence: 99%

Reversed phase HPLC determination of zidovudine in rat plasma and its pharmacokinetics after a single intranasal dose administration

Mainardes

Gremião

2009

Biol. Res.

View full text Add to dashboard Cite

The development and validation of a simple and accurate method based on HPLC with ultraviolet detection for the quantification of zidovudine in rat plasma and its application to a pharmacokinetic study following a single intranasal dose zidovudine is described. Zidovudine was extracted from the plasma using a single-step deproteinization. Chromatographic separation of zidovudine from interfering components was achieved with a C-18 reverse phase column, a mobile phase consisting of a mixture of sodium acetate buffer (55mM) with pH adjusted to 7.0 and acetonitrile (91: 9 v/v) and UV detection set at 265 nm. The method was linear from 100 to 10000 ng.mL -1 (r 2 ≥ 0.9995), and zidovudine had a mean recovery from plasma of 92.8%. The coefficient of variation of inter-day and intra-day quality control samples was less than 15%. After a single intranasal dose of zidovudine administered to rats, pharmacokinetic parameters (AUC 0-24 , C max , t max , t 1/2 ) were determined. The proposed method was found to be simple, specific, accurate, and precise and could be applied to the quantitative analysis of clinical pharmacokinetic studies of zidovudine in rats.

show abstract

“…Several analytical techniques have been reported for the determination of nevirapine [15][16][17][18][19][20][21][22][23][24][25]. The method in the United States Pharmacopoeia (USP)-monograph for determining nevirapine and its related compounds, A and B-uses a reversed-phase separation with UV detection [26].…”

Section: Nevirapinementioning

confidence: 99%

Spectrophotometric determination of nevirapine using tetrathiocyanatocobalt(II) ion as a reagent

Badiadka

Narayana

2010

Eclet. Quim.

View full text Add to dashboard Cite

A simple and rapid spectrophotometric method for the determination of nevirapine is described. The method is based on the reaction of nevirapine with tetrathiocyanatocobalt(II) ion in buffer of pH 4 to form the corresponding complex. Beer’s law is obeyed in the range of 0.2 – 2.0 μg mL-1 for nevirapine. The optical parameters such as molar absorptivity, Sandell’s sensitivity, detection limit and quantitation limit were found to be 1.16× 104 Lmol-1cm-1, 2.09 X 10-3 μg cm-2, 0.073 μg mL-1 and 0.222 μg mL-1 respectively. The optimum reaction conditionsand other analytical parameters were evaluated. The statistical evaluation of the method was examined by determining intra-day and inter-day precision. The proposed method has been successfully applied for the determination of nevirapine in pharmaceutical formulations.

show abstract

A simple and rapid liquid chromatography method for simultaneous determination of zidovudine and nevirapine in plasma

Cited by 42 publications

References 24 publications

Electrochemical studies of nevirapine, an anti-HIV drug, and its assay in tablets and biological samples

Electrochemical studies of nevirapine, an anti-HIV drug, and its assay in tablets and biological samples

Reversed phase HPLC determination of zidovudine in rat plasma and its pharmacokinetics after a single intranasal dose administration

Spectrophotometric determination of nevirapine using tetrathiocyanatocobalt(II) ion as a reagent

Contact Info

Product

Resources

About