A simple and rapid assay to evaluate purity of foot-and-mouth disease vaccine before animal experimentation

Kim, Hye-Jin; Kim, Ah-Young; Kim, Jae‐Seok; Lee, Jungmin; Lee, Hyeyoung; Cheong, Kwang-Myun; Kim, Byounghan; Park, Choi‐Kyu; Ko, Young-Joon

doi:10.1016/j.vaccine.2019.05.053

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…Furthermore, this purity is also important for the Good Manufacturing Process certification of a FMD vaccine. For this reason, the new in-process tests have been developed in recent years [11,12]. These tests were capable of the determination of NSPs during the process (in-process tests).…”

Section: Discussionmentioning

confidence: 99%

“…As an alternative to this in-vivo test, two test kit developed to use in-process. At first, the filtration-assisted chemiluminometric enzyme-linked immunosorbent assay (FAL-ELISA) method and as a second, a newly developed test kit based on lateral flow assay device using monoclonal antibody against the 3B NSP [11,12].…”

Section: Determination Of Non-structural Protein Level For Turkey Foomentioning

confidence: 99%

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Determination of non-structural protein level for Turkey foot-and-mouth disease vaccine antigens during in-process

Sareyyüpoğlu

Çokçalışkan

Çoskuner

et al. 2020

Clin Exp Vaccine Res

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Foot-and-mouth disease (FMD) is a highly contagious viral disease which affects the cloven-hooved animals causing serious economic losses [1]. FMD virus belongs to the family Picornaviridae. It has an approximately 8 kB RNA genome encoding a single polyprotein that is cleaved into 12 different proteins by the help of viral proteases. Among these proteins, there are structural virus capsid proteins (SPs); VP1-4, and the non-structural proteins (NSPs); L, 2A, 2B, 2C, 3A, 3B, 3C, and 3D. Since the NSPs are clarified during the vaccine production, vaccinated animals elicit antibodies only against SPs, but FMD infected animals elicit antibodies against both SPs and NSPs [1-3]. Developed from this principle, Differentiating Infected from Vaccinated Animals (DIVA) tests are differentiated the vaccinated animal from the infected one. These tests are used in the serosurveilance activities of endemic countries, and have been

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Section: Discussionmentioning

confidence: 99%

Section: Determination Of Non-structural Protein Level For Turkey Foomentioning

confidence: 99%

Determination of non-structural protein level for Turkey foot-and-mouth disease vaccine antigens during in-process

Sareyyüpoğlu

Çokçalışkan

Çoskuner

et al. 2020

Clin Exp Vaccine Res

View full text Add to dashboard Cite

show abstract

Development of a double-antibody sandwich ELISA for rapidly quantitative detection of residual non-structural proteins in inactivated foot-and-mouth disease virus vaccines

Liu

Cao

et al. 2023

Journal of Virological Methods

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In-process quality control in foot-and-mouth disease vaccine production by detection of viral non-structural proteins using chemiluminescence dot blot assay

Jabeen,

Bisht,

Ranjitha

et al. 2024

Journal of Virological Methods

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A simple and rapid assay to evaluate purity of foot-and-mouth disease vaccine before animal experimentation

Cited by 4 publications

References 17 publications

Determination of non-structural protein level for Turkey foot-and-mouth disease vaccine antigens during in-process

Determination of non-structural protein level for Turkey foot-and-mouth disease vaccine antigens during in-process

Development of a double-antibody sandwich ELISA for rapidly quantitative detection of residual non-structural proteins in inactivated foot-and-mouth disease virus vaccines

In-process quality control in foot-and-mouth disease vaccine production by detection of viral non-structural proteins using chemiluminescence dot blot assay

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