A simple and quick technique for establishing axenic cultures of the centric diatom <i>Coscinodiscus wailesii</i> Gran

Nagai, Satoshi; Imai, Ichiro; Manabe, Takehiko

doi:10.1093/plankt/20.7.1417

Cited by 18 publications

(11 citation statements)

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“…Авторы пришли также к заключению, что вертикальная миграция не яв-ляется основным фактором, определяющим наблюдаемое накопление ЭПС на свету. Поскольку в аксенических культурах C. closterium отсутст-вовали бактерии (Nagai et al, 1998 ), деградация ЭПС в этих условиях может быть связана с активностью только тех гидролитических фермен-тов, которые секретируются клетками этих водорослей (Staats et al, 2000).…”

Section: влияние освещения на формирование экзополисахаридовunclassified

Diatom exopolysaccharides. A review

Shniukova¹

2015

Algologia

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Section: влияние освещения на формирование экзополисахаридовunclassified

Diatom exopolysaccharides. A review

Shniukova¹

2015

Algologia

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“…The strains are clonal and axenic by micropipetting and washing according to the method of Nagai et al (1998). The stock cultures were maintained in 18 mm Â 180 mm glass test tubes containing 18 ml of modified MP1 medium (Nagai and Manabe, 1993) at 15 8C under 35 mmol m À2 s À1 of cool-white fluorescent illumination on a 10:14 h LD cycle.…”

Section: Organisms and Culture Conditionsmentioning

confidence: 99%

Effect of temperature on light-limited growth of the harmful diatom Coscinodiscus wailesii, a causative organism in the bleaching of aquacultured Porphyra thalli

Nishikawa

Yamaguchi

2008

Harmful Algae

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“…Three strains, A-6, D-14 and F-6, are clonal and were made axenic by micro-pipetting and washing according to the method of Nagai et al (1998). The other strain, H31-C is also clonal but not axenic.…”

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confidence: 99%

Cell size reduction and restoration of the diatom <i>Eucampia zodiacus</i> in culture

Nishikawa

2017

Plankton Benthos Res

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Abstract:We investigated the cell size reduction and size restoration of Eucampia zodiacus, an organism causing bleaching of aquacultured nori seaweed (Pyropia yezonensis), in laboratory culture observations. Mean cell size (apical length) of four E. zodiacus strains, which at the beginning of the experiment ranged between 59.2-73.4 µm, decreased to approximately 10 µm after 265-315 days with increasing number of cell divisions. The mean reduction rates in cell size ranged from 0.04 to 0.28 µm division -1 at 5 µm intervals of cell size. The rates were almost stable at cell sizes between 35 and 60 µm, and then declined with decreasing cell size. When the mean cell sizes reached <15 µm, they restored cell size. However, the restored-size cells ranged from 22.7-34.0 µm and were smaller than the maximum size (ca. 80 µm) observed in field samples from the Harima-Nada, eastern part of the Seto Inland Sea, Japan. Cell size restored to over 80 µm was observed only once, in the H31-C strain, when the mean cell size decreased to around 50 µm. The process of size restoration that was observed only once in the H31-C strain, was clearly different from the other size restoration mechanisms, suggesting that E. zodiacus has two different processes by which cell size is restored: sexual reproduction via auxospore formation and vegetative cell enlargement.

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A simple and quick technique for establishing axenic cultures of the centric diatom Coscinodiscus wailesii Gran

Cited by 18 publications

References 0 publications

Diatom exopolysaccharides. A review

Diatom exopolysaccharides. A review

Effect of temperature on light-limited growth of the harmful diatom Coscinodiscus wailesii, a causative organism in the bleaching of aquacultured Porphyra thalli

Cell size reduction and restoration of the diatom <i>Eucampia zodiacus</i> in culture

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