A simple and practical solvent system selection strategy for high-speed countercurrent chromatography based on the HPLC polarity parameter model

Zhu, Xiaomeng; Li, Pengcheng; Tang, Jintian; Su, Yanqi; Xiao, Mi; Xue, Huang; Cai, Xu

doi:10.1039/d2ay01377k

Cited by 4 publications

(3 citation statements)

References 30 publications

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“…Considering the influence of the polarity of the solute, stationary phase, and mobile phase on k, another linear model was proposed to accurately describe k, which represents the linear relationship between the retention rate and the polarity of the eluent (Gisbert-Alonso et al, 2021;Zhu et al, 2022); the relationship is as follows:…”

Section: Influence Of the Organic Modifier Compositionmentioning

confidence: 99%

“…For the water-methanol system, the relationship between P N m and φ is as follows (Zhu et al, 2022):…”

Section: Influence Of the Organic Modifier Compositionmentioning

confidence: 99%

“…Considering the influence of the polarity of the solute, stationary phase, and mobile phase on k, another linear model was proposed to accurately describe k, which represents the linear relationship between the retention rate and the polarity of the eluent ( Gisbert-Alonso et al, 2021 ; Zhu et al, 2022 ); the relationship is as follows:

where p is the parameter describing the polarity of the solute,

and

are the standard polarity parameters of the mobile and stationary phases, and

is the retention factor when the polarity of the mobile phase is the same as that of the stationary phase. Numerous experimental studies have shown that for a specific column and water-methanol mobile phase, the parameters of Eq.…”

Section: Theorymentioning

confidence: 99%

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Simultaneous effect of different chromatographic conditions on the chromatographic retention of pentapeptide derivatives (HGRFG and NPNPT)

et al. 2023

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Introduction: Oligopeptides exhibit great prospects for clinical application and its separation is of great importance in new drug development.Methods: To accurately predict the retention of pentapeptides with analogous structures in chromatography, the retention times of 57 pentapeptide derivatives in seven buffers at three temperatures and four mobile phase compositions were measured via reversed-phase high-performance liquid chromatography. The parameters (kHA, kA, and pKa) of the acid–base equilibrium were obtained by fitting the data corresponding to a sigmoidal function. We then studied the dependence of these parameters on the temperature (T), organic modifier composition (φ, methanol volume fraction), and polarity (PmN parameter). Finally, we proposed two six-parameter models with (1) pH and T and (2) pH and φ or PmN as the independent variables. These models were validated for their prediction capacities by linearly fitting the predicted retention factor k-value and the experimental k-value.Results: The results showed that logkHA and logkA exhibited linear relationships with 1/T, φ or PmN for all pentapeptides, especially for the acid pentapeptides. In the model of pH and T, the correlation coefficient (R2) of the acid pentapeptides was 0.8603, suggesting a certain prediction capability of chromatographic retention. Moreover, in the model of pH and φ or PmN, the R2 values of the acid and neutral pentapeptides were greater than 0.93, and the average root mean squared error was approximately 0.3, indicating that the k-values could be effectively predicted.Discussion: In summary, the two six-parameter models were appropriate to characterize the chromatographic retention of amphoteric compounds, especially the acid or neutral pentapeptides, and could predict the chromatographic retention of pentapeptide compounds.

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Section: Influence Of the Organic Modifier Compositionmentioning

confidence: 99%

“…For the water-methanol system, the relationship between P N m and φ is as follows (Zhu et al, 2022):…”

Section: Influence Of the Organic Modifier Compositionmentioning

confidence: 99%

“…Considering the influence of the polarity of the solute, stationary phase, and mobile phase on k, another linear model was proposed to accurately describe k, which represents the linear relationship between the retention rate and the polarity of the eluent ( Gisbert-Alonso et al, 2021 ; Zhu et al, 2022 ); the relationship is as follows:

where p is the parameter describing the polarity of the solute,

and

are the standard polarity parameters of the mobile and stationary phases, and

Section: Theorymentioning

confidence: 99%

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Simultaneous effect of different chromatographic conditions on the chromatographic retention of pentapeptide derivatives (HGRFG and NPNPT)

et al. 2023

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Separation of astragaloside IV from Astragalus membranaceus based on high‐speed countercurrent chromatography in continuous injection mode

Zhao,

Su,

Wang

et al. 2024

Phytochemical Analysis

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IntroductionAstragaloside IV (AS‐IV) is an index for the quality evaluation of the traditional Chinese medicine Astragalus and an important material basis for Astragalus to exert its medicinal effects, and it is difficult to obtain a single AS‐IV by ordinary separation methods.ObjectiveTo find a new isolation method that can prepare AS‐IV quickly and efficiently.MethodologyAS‐IV was isolated from Astragalus membranaceus extract by high‐speed countercurrent chromatography using a two‐phase solvent system consisting of ethyl acetate/n‐butanol/water (4.2:0.8:5, v/v) at a speed of 950 rpm at a flow rate of 2 mL/min using one of the high‐speed countercurrent chromatographic sequential injection models developed during the previous study.ResultsCompared with the common countercurrent chromatographic separation, this separation method increased the injection volume and yield by 4‐fold and 4.47‐fold, respectively, with only about 1.2‐fold increase in solvent consumption and separation time, and the purity was basically not reduced, and 55.9 mg of AS‐IV, with a purity of 96.95%, was finally prepared from 400 mg of the crude extract in 240 min.ConclusionThe continuous injection mode of high‐speed countercurrent chromatography was able to successfully prepare a large amount of AS‐IV with high purity at one time.

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Improvement of polarity-based solvent system for countercurrent chromatography in the guidance of solvent selectivity: n-hexane/ethyl acetate/ alcohol solvents/water as an example

Luan,

Sun,

Yang

et al. 2024

Journal of Chromatography A

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A simple and practical solvent system selection strategy for high-speed countercurrent chromatography based on the HPLC polarity parameter model

Abstract: The selection of appropriate solvent system was the most crucial step of high-speed countercurrent chromatography (HSCCC) separation. The compound polarity played an important role in HPLC analysis and HSCCC separation,...

Cited by 4 publications

References 30 publications

Simultaneous effect of different chromatographic conditions on the chromatographic retention of pentapeptide derivatives (HGRFG and NPNPT)

Simultaneous effect of different chromatographic conditions on the chromatographic retention of pentapeptide derivatives (HGRFG and NPNPT)

Separation of astragaloside IV from Astragalus membranaceus based on high‐speed countercurrent chromatography in continuous injection mode

Improvement of polarity-based solvent system for countercurrent chromatography in the guidance of solvent selectivity: n-hexane/ethyl acetate/ alcohol solvents/water as an example

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