A simple and non-invasive method for analyzing local immune responses in vivo using fish fin

Matsuura, Yuta; Takaoka, Naoki; Miyazawa, Ryuichiro; Nakanishi, Teruyuki

doi:10.1016/j.dci.2017.04.016

Cited by 6 publications

(5 citation statements)

References 37 publications

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“…3). This technique allowed us to investigate phagocytic and respiratory burst activities of granulocytes in vivo without any in vitro cellular treatment [50]. We obtained similar results following injection of Staphylococcus aureus bacterial antigen (unpublished data).…”

Section: Percutaneous Administration Via Stamp Injectionmentioning

confidence: 53%

Mucosal delivery of fish vaccines: Local and systemic immunity following mucosal immunisations

Somamoto

Nakanishi²

2020

Fish & Shellfish Immunology

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The mucosal organs of fishes are directly exposed to their aquatic environment, which is suited to the colonization and growth of microorganisms, and thus these barriers are considered to play an important role in maintaining homeostasis and preventing entry of invasive pathogens. Research on fish mucosal immunity have shown that mucosal organs such as gills, skin, intestines, and olfactory organs harbor lymphoid cells, including T and B cells as well as dendritic-like cells. Findings related to immune responses following direct administration of antigens into the mucosal organs could help to shed light upon the development of fish mucosal vaccines. The present review highlights vaccine delivery via mucosal organs, in particular focusing on methods other than those of typical mucosal vaccine platforms, such as oral and immersion vaccines. In addition, we propose the hypothesis that mucosal tissues are important sites for generating cell-mediated immunity following vaccination with extracellular antigens.

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Section: Percutaneous Administration Via Stamp Injectionmentioning

confidence: 53%

Mucosal delivery of fish vaccines: Local and systemic immunity following mucosal immunisations

Somamoto

Nakanishi²

2020

Fish & Shellfish Immunology

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“…Intra-fin administration has been developed as a simple and noninvasive method to analyze local immune responses in vivo [6]. We considered this a suitable method to analyze local immune responses to the infective stage of the parasites, which settles in the epithelial tissue.…”

Section: Resultsmentioning

confidence: 99%

“…Intra-fin administration of the antigens was conducted according to the method described by Matsuura et al [6], with slight modification for a parasite antigen. Briefly, the theronts were suspended and adjusted to 10,000 cells/200 μL in phosphate-buffered saline (PBS).…”

Section: Intra-fin Administrationmentioning

confidence: 99%

“…The cells were washed with OPTI-MEM. CD8 + T, CD4 + T, IgM + , and macrophages/granulocytes were detected by flow cytometry (FCM) following methods reported previously [6,10,11]. CD8 + T, CD4 + (CD4-1 + ) T, and IgM + cells were stained with monoclonal antibodies (mAb; rat mAb, 2C3, and 6D1; and mouse mAb, B12, and GB21, respectively).…”

Section: Preparation Of Fin Leukocytes and Flow Cytometry Analysesmentioning

confidence: 99%

“…Although information on immunity against I. multifiliis is gradually accumulated in several fish species, the in vivo immune responses to the stages from theronts to trophonts still remain unclear. Recently, a novel method to analyze in vivo immune responses using fish fin has been established in ginbuna crucian carp, and has shown in vivo immune functions, such as phagocytosis [6] and lymphoblasts [7]. As this technique provides a unique tool that could help better understand the mechanisms of local immune responses to antigens in epithelium tissues, we considered it necessary to monitor in vivo the immune responses to I. multifiliis.…”

Section: Introductionmentioning

confidence: 99%

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Local immune responses to two stages of Ichthyophthirius multifiliis in ginbuna crucian carp

Shiota

Sukeda

Prakash

et al. 2021

Fish & Shellfish Immunology

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Induction of both local and systemic immunity by in vivo injection of PHA into ginbuna carp fin

Miyazawa

Iijima

Nakanishi

2022

Developmental & Comparative Immunology

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A simple and non-invasive method for analyzing local immune responses in vivo using fish fin

Cited by 6 publications

References 37 publications

Mucosal delivery of fish vaccines: Local and systemic immunity following mucosal immunisations

Mucosal delivery of fish vaccines: Local and systemic immunity following mucosal immunisations

Local immune responses to two stages of Ichthyophthirius multifiliis in ginbuna crucian carp

Induction of both local and systemic immunity by in vivo injection of PHA into ginbuna carp fin

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