A simple and inexpensive procedure for detection of a marker of Brazilianalecrimpropolis

Abstract

The detection of marker compounds is a necessary step for chemical characterization and standardization of propolis. A thin-layer chromatography procedure is described for the detection of allyl 3-prenylcinnamate, a volatile marker of Brazilian green propolis. The powdered sample is heated inside a petri dish and the material condensed on the dish cap is chromatographed on silica gel plates. The visualization may be done by spraying the plates with a solution of sodium fluorescein or a solution of sulphuric acid vanillin reagent.Un procedimiento sencillo y barato para la detecció n de un marcador del propó leo verde brasileñ o.La detecció n de compuestos marcadores es un paso necesario para la caracterizació n química y la estandarizació n de los propó leos. Un procedimiento de analisis por cromatografia de capa fina se describe para la detecció n de 3-alilprenilcinamato, un marcador volátil del propó leo verde brasileño. Una muestra en polvo se calienta en el interior de una placa de Petri. Los materiales que se condensan en la tapa se cromatografian sobre placas de gel de sílice. La visualizació n se hace pulverizando las placas con una solució n de fluoresceína de sodio o del reactivo vainillina/ácido sulfúrico.

Identification of Free Radical Scavengers from Brazilian Green Propolis Using Off‐Line HPLC‐DPPH Assay and LC‐MS

“…Its characteristic constituents have found to be prenyled phenylpropanoids, caffeoylquinic acids, and diterpenoid acids (Salatino and others ). Many analytical separation techniques have been used to analyze these compounds, including TLC (thin‐layer chromatography), high‐performance liquid chromatography (HPLC), gas chromatography (GC), and chromatography combined with mass spectrometry (MS; Midorikawa and others ; Bankova ; de Sousa and others ; Matsuda and de Almeida‐Muradian ; Mayworm and others ). For the past several years, chromatographic fingerprints have been used to characterize the chemical profiles to identify plant origin, authentication, and quality.…”

A Novel Sensitive Luminescence Probe Microspheres for Rapid and Efficient Detection of τ-Fluvalinate in Taihu Lake

“…Many works use it to detect target through the fluorescence changes4950. Figure S2 showed the synthesizing chemical equation of the allyl fluorescein.…”

“…Allyl fluorescein as a kind of fluorochrome was chosen with a simple and convenient method, which provided group of vinyl to reacted with cross-linking agent and monomer and form the polymer layer 48 . Many works use it to detect target through the fluorescence changes 49 50 . Figure S2 showed the synthesizing chemical equation of the allyl fluorescein.…”

How diverse is the chemistry and plant origin of Brazilian propolis?

“…A second marker has been proposed, namely, the allyl ester of 3-prenyl-cinnamic acid (Fig. 3B) (Nascimento et al 2008;Mayworm et al 2015). Chemical markers of Brazilian red propolis are isoflavones, e.g., formononetin (Fig.…”