A simple and highly sensitive UPLC-ESI-MS/MS method for the simultaneous quantification of nicotine, cotinine, and the tobacco-specific carcinogens N’-nitrosonornicotine and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in serum samples

Loukotková, Lucie; VonTungeln, Linda S.; Vanlandingham, Michelle; Costa, Gonçalo Gamboa da

doi:10.1016/j.jchromb.2017.11.022

Cited by 14 publications

(24 citation statements)

References 15 publications

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“…Methanol failed to obtain good peak shapes simultaneously for nicotine and cotinine. In accordance with four published studies, good peak shapes were simultaneously obtained for both analytes using acetonitrile (containing 0.1% formic acid) and ammonium formate (Abdallah et al, ; Dobrinas et al, ; Loukotkova et al, ; Mao et al, ).…”

Section: Discussionsupporting

confidence: 86%

“…The quantitative and qualitative ion pairs for both two analytes and IS were similar to those reported in previous methods (Abdallah et al, ; Dobrinas et al, ; Liachenko et al, ; Loukotkova et al, ; Mao et al, ; Miller et al, ; Shakleya & Huestis, ; Stolker et al, ; Yuan et al, ). The 10 μL sample volume was comparable with that in two published methods (10–20 μL) (Kaisar et al, ; Loukotkova et al, ) and much lower than those used in other studies (200–1000 μL) (Baumann et al, ; Dobrinas et al, ; Liachenko et al, ; Miller et al, ; Shakleya & Huestis, ; Stolker et al, ; Yuan et al, ). The 1 μL injection volume was lower than those used in all previous studies (2–30 μL) (Abdallah et al, ; Abu‐Awwad et al, ; Baumann et al, ; Dobrinas et al, ; Kaisar et al, ; Loukotkova et al, ; Mao et al, ; Shakleya & Huestis, ; Stolker et al, ; Yuan et al, ).…”

Section: Discussionsupporting

confidence: 85%

“…The 10 μL sample volume was comparable with that in two published methods (10–20 μL) (Kaisar et al, ; Loukotkova et al, ) and much lower than those used in other studies (200–1000 μL) (Baumann et al, ; Dobrinas et al, ; Liachenko et al, ; Miller et al, ; Shakleya & Huestis, ; Stolker et al, ; Yuan et al, ). The 1 μL injection volume was lower than those used in all previous studies (2–30 μL) (Abdallah et al, ; Abu‐Awwad et al, ; Baumann et al, ; Dobrinas et al, ; Kaisar et al, ; Loukotkova et al, ; Mao et al, ; Shakleya & Huestis, ; Stolker et al, ; Yuan et al, ). In fact, a 2 μL injection volume will result in significant column overload and appearance of a leading peak.…”

Section: Discussionsupporting

confidence: 66%

“…In fact, a 2 μL injection volume will result in significant column overload and appearance of a leading peak. The one‐step protein precipitation is simple and efficient for application, and is also used in many studies (Baumann et al, ; Dobrinas et al, ; Kaisar et al, ; Loukotkova et al, ; Miller et al, ; Onoue et al, ; Stolker et al, ). The whole running time for a single sample was about 4 min (0.4 min sampling time and 3.6 min run time), which was shorter than those reported in most studies (5.5–20 min) (Abdallah et al, ; Baumann et al, ; Dobrinas et al, ; Loukotkova et al, ; Mao et al, ; Miller et al, ; Onoue et al, ), but longer than those in four studies (2.4–3.4 min) (Abu‐Awwad et al, ; Kaisar et al, ; Liachenko et al, ; Yuan et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Various methods have been developed to determine nicotine and cotinine in plasma, including HPLC, HPLC–MS/MS, and GC–MS (Abdallah, Hammell, Stinchcomb, & Hassan, ; Abu‐Awwad, Arafat, & Schmitz, ; Baumann, Regenthal, Burgos‐Guerrero, Hegerl, & Preiss, ; Dobrinas et al, ; Earla, Ande, McArthur, Kumar, & Kumar, ; Jacob et al, ; Kaisar et al, ; Liachenko, Boulamery, & Simon, ; Loukotkova, VonTungeln, Vanlandingham, & da Costa, ; Mao et al, ; Miller, Norris, Rollins, Tiffany, & Wilkins, ; Onoue, Yamamoto, Seto, & Yamada, ; Shakleya & Huestis, ; Stolker, Niesing, Hogendoorn, Bisoen Rambali, & Vleeming, ; Xu et al, ; Yuan et al, ; Yuan, Kosewick, & Wang, ). However, there are many deficiencies in these published methods, such as the large sample volume requirement (200–1000 μL; Baumann et al, ; Dobrinas et al, ; Liachenko et al, ; Miller et al, ; Shakleya & Huestis, ; Stolker et al, ; Yuan et al, ), complicated and time‐consuming sample pretreatment (Abdallah et al, ; Shakleya & Huestis, ), the long analysis time (5.5–20 min; Abdallah et al, ; Baumann et al, ; Dobrinas et al, ; Loukotkova et al, ; Mao et al, ; Miller et al, ; Onoue et al, ) and poor peak shape (Baumann et al, ). Therefore, this study aims to develop and validate a fast, accurate and robust ultra‐high‐performance liquid chromatography tandem mass/mass spectrometry (UHPLC–MS/MS) method for simultaneous determination of plasma nicotine and cotinine in mice, and apply it to a pharmacokinetic study.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous determination of plasma nicotine and cotinine by UHPLC–MS/MS in C57BL/6 mice and its application in a pharmacokinetic study

Liu

Zhang

et al. 2019

Biomedical Chromatography

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Plasma concentrations of nicotine and its active metabolite cotinine are highly correlated with its biological effects. A UHPLC-MS/MS method was developed, validated and applied for nicotine and cotinine analysis in mice plasma. Chromatographic separation was achieved on a BEH HILIC column using acetonitrile (0.1% formic acid) and 10 mM ammonium formate as mobile phase. The gradient elution was performed at 0.4 mL/min with a run time of 3.6 min. The quantitative ion transition was m/z 163.1 > 130.0 for nicotine, m/z 177.1 > 80.0 for cotinine and m/z 167.1 > 134.0 for nicotine-D 4 (internal standard, IS). For both nicotine and cotinine, the calibration range was 5-500 ng/mL with 5 ng/mL as the lower limit of quantitation, and the intra-and inter-day bias and imprecision were −4.61-12.00% and <11.12%. The IS normalized recovery was 90.62-98.95% for nicotine and 89.18-101.53% for cotinine, and the IS normalized matrix factor was 106.00-116.44% for nicotine and 100.34-109.85% for cotinine. Both nicotine and cotinine were stable under conventional storage conditions. The validated method has been applied to a pharmacokinetic study in mice to calculate the pharmacokinetic parameters for both analytes. KEYWORDS cotinine, method development and validation, nicotine, pharmacokinetics, UHPLC-MS/MS Abbreviations used: UHPLC-MS/MS, ultra-high-performance liquid chromatography tandem mass/mass spectrometry; QC, quality control; LLOQ, lowest limit of quantitation; CV, coefficient of variation *Yang Liu and Dongjie Zhang equal first authors.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Simultaneous determination of plasma nicotine and cotinine by UHPLC–MS/MS in C57BL/6 mice and its application in a pharmacokinetic study

Liu

Zhang

et al. 2019

Biomedical Chromatography

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Review of HPLC–MS methods for the analysis of nicotine and its active metabolite cotinine in various biological matrices

Jin

Pang

Zhao

et al. 2022

Biomedical Chromatography

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In recent years, tobacco smoking is a risk factor for a series of diseases, including cardiovascular diseases, cerebrovascular diseases, and cancers. Nicotine, the primary component of tobacco smoke, is mainly transformed to its active metabolite cotinine, which is often used as a biomarker for tobacco exposure for its higher blood concentration and longer residence time than nicotine. Various analytical methods have been developed for the determination of nicotine and cotinine in biological matrices. This article reviewed the HPLC-MS based methods for nicotine and/or cotinine analysis in various biological matrices. The sample preparation, mass and chromatographic conditions, and method validation results of these methods have been summarized and analyzed. The sample was mainly pretreated by protein precipitation and/or extraction.Separation was achieved using methanol and/or acetonitrile:water (with or without ammonium acetate) on C18 columns and acetonitrile:water (with formic acid, ammonium acetate/formate) on HILIC columns. Nicotine-d3, nicotine-d4, and cotinine-d3 were commonly used internal standards (ISs). Other non-deuterated ISs such as ritonavir, N-ethylnorcotinine, and milrinone were also used. For both nicotine and cotinine, the calibration range was 0.005-35,000 ng/mL, the matrix effect was 75.96-126.8%, and the recovery was 53-124.5%. The two analytes were stable at room temperature for 1-10 days, at À80 C for up to 6 months, and after three to six freeze-thaw cycles.Comedications did not affect nicotine and cotinine analyses.

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Brain distribution and metabolic profiling of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in rats investigated by UHPLC-HRMS/MS following peripheral administration

et al. 2023

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A simple and highly sensitive UPLC-ESI-MS/MS method for the simultaneous quantification of nicotine, cotinine, and the tobacco-specific carcinogens N’-nitrosonornicotine and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in serum samples

Cited by 14 publications

References 15 publications

Simultaneous determination of plasma nicotine and cotinine by UHPLC–MS/MS in C57BL/6 mice and its application in a pharmacokinetic study

Simultaneous determination of plasma nicotine and cotinine by UHPLC–MS/MS in C57BL/6 mice and its application in a pharmacokinetic study

Review of HPLC–MS methods for the analysis of nicotine and its active metabolite cotinine in various biological matrices

Brain distribution and metabolic profiling of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in rats investigated by UHPLC-HRMS/MS following peripheral administration

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