A Simple and Highly Reproducible Staining Method for Fungi and Other Polysaccharide-Rich Microorganisms in Animal Tissues

Tome, Yoshiya; Hayashi, I.; Matsuoka, Setsuko; Yamamoto, Mitsue; Upton, Melissa P.; Hirohashi, Setsuo; Shimosato, Yukío

doi:10.3109/10520298809107160

Cited by 8 publications

(2 citation statements)

References 12 publications

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“…For the light microscopic demonstration of microorganisms, 5 μm plastic sections were cut with a motor driven rotation microtome (Autocut, Reichert-Jung), and stained for Gram-positive and Gram-negative bacteria with the modified method of Brown and Brenn (1931), and for fungi a modification of Grocott's silver method (Tome et al 1988) was additionally applied.…”

Section: Methodsmentioning

confidence: 99%

Demonstration of β-glucan receptors in the skin of aquatic mammals—a preliminary report

Meyer

Kloepper²,

Fleischer

2008

Eur J Wildl Res

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Using immunohistochemistry, the study clearly demonstrates three important β-glucan receptors (Ficolin/ P35, MBL, Dectin-1; members of the lectin-complement pathway of innate immunity) in the integument of six marine and freshwater aquatic mammals (Northern fur seal, Common seal, Walrus, Coypu, Capybara, Otter), but only weakly in two dolphin species. Most of the non-dolphin mammals exhibited strong reactions, especially with regard to the skin glands (tubular apocrine glands, sebaceous glands), for L-Ficolin/P35 and MBL. Distinct reaction staining could also be observed in the epidermis and the outer epithelial sheath of primary hair follicles.Positive Dectin-1 staining was limited to secretory cells of the apocrine tubular glands, and to peripheral and central cells of sebaceous glands of the seals. The Capybara was the only animal to show a clear Dectin reaction in the epidermis (stratum granulosum).The findings are discussed with regard to the constant and high microbial challenge of the skin in the aquatic medium, and variations in hair density of the animals.

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Section: Methodsmentioning

confidence: 99%

Demonstration of β-glucan receptors in the skin of aquatic mammals—a preliminary report

Meyer

Kloepper²,

Fleischer

2008

Eur J Wildl Res

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“…They were stained with haematoxylin and eosin. Selected sections were stained by the Gram method (Leaver et al, 1977), Grocott's method (Tome et al, 1988) and the Kossa method.…”

Section: Histopathologymentioning

confidence: 99%

Department of Animal Health, Tokyo University of Agriculture, Tokyo, Japan

Murakami

Azuma

Oomi

et al. 1999

Journal of Veterinary Medicine Series A

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The present experiment was performed to test the pathogenicity of Actinomyces‐like bacteria in experimental animals and swine. Two rough (R) strains of Actinomyces‐like bacteria isolated from a site of arthritis and from the tonsil in pigs were used as inocula. To investigate their susceptibility to Actinomyces‐like bacteria, BALB/c, SS and ddY mice and guinea‐pigs were inoculated intraperitoneally with the strains of Actinomyces‐like bacteria. The ddY mice were used for the long‐term observation of Actinomyces‐like bacteria lesions and the mammary tissue of a sow was inoculated with Actinomyces‐like bacteria isolated from swine tonsil. Macroscopic observation revealed many abscesses on the surfaces of the abdominal and/or thoracic organs in the mice, but not on those of the guinea‐pigs. The sow developed firm nodules at the inoculation site in the mammary glands. Histopathologically, the lesions in the mice were characterized as actinomycotic abscesses in the early stage and as pus‐forming granuloma (PFG) in the advanced stage; the lesions were accompanied by crystalloid particles. Actinomyces‐like bacteria induced granulomatous mastitis in the sow, and the lesion was characterized as PFG. The characteristic actinomycotic lesions in swine mammary glands were reproduced by experimental infection.

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Murakami

Azuma

Koeda³

et al. 1998

Mycopathologia

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The tonsils of eleven pigs and the mammary glands of a sow were used to investigate actinomycotic lesions due to Actinomyces sp. infection. At necropsy, there was no abnormality on these tonsils, on the other hand, numerous abscesses containing sulfur granules were found in the mammary. Histopathologically, the Actinomyces sp. lesions were noted as crypt abscesses in the tonsils and as pus-forming granulomas in the mammary glands. The microorganisms in both lesions were composed of bead-like cocci, bacillary cells and short, branching filaments, those cells being positive by the Gram's and Grocott's methods. Clubs were formed around the microbial clumps in these lesions. Immunohistochemically, there were cross-reactions between antibodies of Actinomyces sp. Chiba 101 (101) and swine actinomycetes of 7 species: A. bovis, A. hyovaginalis, A. israeli, A. naeslundii, A. pyogenes, A. suis) formerly Eubacterium suits) and A. viscosus. However it was possible to differentiate Actinomyces sp. 101 from them by absorption and dilution of the antiserum, then the microorganisms in the tonsillar crypt abscesses and the granulomatous mastitis were labelled with an immunoperoxidase technique using the absorbed Actinomyces sp. 101 antiserum. Thus, these immunolabelling properties are suggestive of the presence of 'A. suis' (Grässer) Franke 1973.

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A Simple and Highly Reproducible Staining Method for Fungi and Other Polysaccharide-Rich Microorganisms in Animal Tissues

Cited by 8 publications

References 12 publications

Demonstration of β-glucan receptors in the skin of aquatic mammals—a preliminary report

Demonstration of β-glucan receptors in the skin of aquatic mammals—a preliminary report

Department of Animal Health, Tokyo University of Agriculture, Tokyo, Japan

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