A simple and fast protocol for the synthesis of 2-amino-4-(4-formylphenyl)-4H-chromene-3-carbonitrile to develop an optical immunoassay for the quantification of botulinum neurotoxin type F

Abstract

In this work, a novel optical immunoassay platform using(S)-2-amino-4-(4-formylphenyl)-4H-chromene-3-carbonitrile as an optical probe which was synthesized by an ultra-sonication method. The structure of the probe was elucidated by Fourier-transform infrared...

First organic fluorescence immunoassay for the detection of Enterobacter cloacae in food matrixes

“…In general, the EDC/NHS coupling protocol results in a covalent bond between the antibody and fluorophores via the formation of amide bonds between them. 33 In our work, amide linkages were also formed between the –COOH– groups of anti- E. cloacae and the –NH 2 group of the ACC fluorophore in the MES buffer (pH = 4.5) medium. To confirm these, we performed both UV-Vis and emission analyses and the changes in absorbance and fluorescence intensity were recorded.…”

“…Finally, the thoroughly washed pellet ACC-anti- E. cloacae was stored at −4 °C in a deep freezer for further sensor studies. 33…”