A simple and fast method for metabolomic analysis by gas liquid chromatography—mass spectrometry

Cosovanu, Diana; Llovera, Montserrat; Villorbina, Gemma; Canela‐Garayoa, Ramon; Eras, Jordi

doi:10.1007/s11306-021-01771-w

Cited by 2 publications

(2 citation statements)

References 39 publications

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“…It is interesting to mention that in almost all of the above described examples in the literature, the studies focused on the characterization of a reduced group of terpenes or even of a single molecule and, therefore, there is not a global approach to identify the expected large number of terpenes from olive leaves. The most recent work published in 2021 presented a metabolomics approach based on GC-MS combined with a simultaneous esterification-silylation reaction; however, the number of terpenes found in olive leaves was rather low (seven triterpenes and one monoterpene) [31]. In the current work, we used gas chromatography coupled to high-resolution mass spectrometry (GC-QTOF-MS) focusing on the identification of terpenes molecules.…”

Section: Introductionmentioning

confidence: 99%

Extraction and Mass Spectrometric Characterization of Terpenes Recovered from Olive Leaves Using a New Adsorbent-Assisted Supercritical CO2 Process

Montenegro

Álvarez‐Rivera

Mendiola

2021

Foods

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This work reports the use of GC-QTOF-MS to obtain a deep characterization of terpenoid compounds recovered from olive leaves, which is one of the largest by-products generated by the olive oil industry. This work includes an innovative supercritical CO2 fractionation process based on the online coupling of supercritical fluid extraction (SFE) and dynamic adsorption/desorption for the selective enrichment of terpenoids in the different olive leaves extracts. The selectivity of different commercial adsorbents such as silica gel, zeolite, and aluminum oxide was evaluated toward the different terpene families present in olive leaves. Operating at 30 MPa and 60 °C, an adsorbent-assisted fractionation was carried out every 20 min for a total time of 120 min. For the first time, GC-QTOF-MS allowed the identification of 40 terpenoids in olive leaves. The GC-QTOF-MS results indicate that silica gel is a suitable adsorbent to partially retain polyunsaturated C10 and C15 terpenes. In addition, aluminum oxide increases C20 recoveries, whereas crystalline zeolites favor C30 terpenes recoveries. The different healthy properties that have been described for terpenoids makes the current SFE-GC-QTOF-MS process especially interesting and suitable for their revalorization.

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Section: Introductionmentioning

confidence: 99%

Extraction and Mass Spectrometric Characterization of Terpenes Recovered from Olive Leaves Using a New Adsorbent-Assisted Supercritical CO2 Process

Montenegro

Álvarez‐Rivera

Mendiola

2021

Foods

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“…EGR is usually measured indoor by using gas chromatography (GC) or mass spectrometry (MS) [18][19][20] but not applied to the field. Flash calculation is one of the basic constituents of gas-liquid equilibrium calculation, which is used to calculate the compositional ratio of gas phase to the liquid phase at a given temperature, and pressure provided the total composition of the system is known [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

A Novel Approach to Determine the Enriched Gas Recycling during the Enriched Gas Flooding Process

Li¹,

Yuan³

2022

Geofluids

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A novel approach was proposed for calculating the enriched gas recovery factor based on the theory of two-phase isothermal flash calculations. First, define a new parameter, pseudo formation volume factor of enriched gas, to represent the ratio of the surface volume of produced mixture gas to underground volume of enriched gas. Two logarithmic functions were obtained by matching the flash calculation data, to characterize the relationships between pseudo formation volume factor and the produced gas-oil ratio. These two functions belong to the proportion of liquefied petroleum gas in enriched gas; the proportion is greater than 50% and less than 50%, respectively. Given measured gas-oil ratio and produced gas volume, underground volume of produced enriched gas can be calculated. Injection volume of enriched gas is known; therefore, recovery factor of enriched gas is the ratio of produced to injected volume of enriched gas. This approach is simply to calculate enriched gas recovery factor, because of only needs three parameters, which can be measured directly. New approach was compared to numerical simulation results; mean error is 12%. In addition, new approach can effectively avoid the influence of lean gas on the calculation of enriched gas recycling. Three stages of enriched gas recovery factors in field Z were calculated, and the mean error is 5.62% compared to the field analysis, which proves that the new approach’s correctness and practicability.

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A simple and fast method for metabolomic analysis by gas liquid chromatography—mass spectrometry

Cited by 2 publications

References 39 publications

Extraction and Mass Spectrometric Characterization of Terpenes Recovered from Olive Leaves Using a New Adsorbent-Assisted Supercritical CO2 Process

Extraction and Mass Spectrometric Characterization of Terpenes Recovered from Olive Leaves Using a New Adsorbent-Assisted Supercritical CO2 Process

A Novel Approach to Determine the Enriched Gas Recycling during the Enriched Gas Flooding Process

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