A simple and facile paper-based colorimetric assay for detection of free hydrogen sulfide in prostate cancer cells

Lee, Jaemyeon; Lee, Young Ju; Ahn, Youngjun; Choi, Samjin; Lee, Gi Ja

doi:10.1016/j.snb.2017.10.019

Cited by 42 publications

(34 citation statements)

References 29 publications

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“…Lee et al [ 73 ] reported a new paper-based colorimetric assay by fabricating a 96-well microplate format for cell culture for sensing H 2 S gas in live cancer cells. Microplate-like hydrophobic walls designed using AutoCAD were printed using a Xerox ColorQube 8570 N printer.…”

Section: Paper-based Colorimetric Assay For Live Cell Analysismentioning

confidence: 99%

Analytical Methods for Detection of Gasotransmitter Hydrogen Sulfide Released from Live Cells

Quan

Lee

2021

BioMed Research International

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Hydrogen sulfide (H2S) plays an important role in mammals as a signaling molecule. Recently, abnormal H2S concentration has been associated with several pathophysiological states, such as diabetes mellitus, hypertension, Alzheimer’s disease, and Parkinson’s disease. As regulating H2S concentration can be a very prominent way of developing new drugs, many researchers have paid great attention to H2S research. To understand the role of H2S in pathophysiology and develop H2S-based therapies, it is necessary to measure the exact concentration of H2S within biological systems. But, H2S is volatile and can be easily oxidized. Besides, the active sites for several biological effects of H2S are inside the cell. Therefore, there is a need for the development of new methods for the accurate and reliable detection of H2S within live cells. This review provides a summary of recent developments in H2S detection methods for live cell analysis.

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Section: Paper-based Colorimetric Assay For Live Cell Analysismentioning

confidence: 99%

Analytical Methods for Detection of Gasotransmitter Hydrogen Sulfide Released from Live Cells

Quan

Lee

2021

BioMed Research International

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“…Several studies investigating non-destructive methods to reliably detect free H 2 S gas in living cells have been reported [16][17][18]. In particular, paper-based colorimetric assays utilizing silver/Nafion™/polyvinylpyrrolidone (PVP) membranes are able to detect quantitatively endogenous H 2 S in live cancer cells without the need for expensive and large instruments or special probes for H 2 S [19]. However, cell-based bioassays to assess the efficacy of anticancer drugs have been evaluated in two-dimensional (2D) cultures of cancer cell lines.…”

Section: Introductionmentioning

confidence: 99%

In Situ Detection of Hydrogen Sulfide in 3D-Cultured, Live Prostate Cancer Cells Using a Paper-Integrated Analytical Device

et al. 2022

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In this study, a paper-integrated analytical device that combined a paper-based colorimetric assay with a paper-based cell culture platform was developed for the in situ detection of hydrogen sulfide (H2S) in three-dimensional (3D)-cultured, live prostate cancer cells. Two kinds of paper substrates were fabricated using a simple wax-printing methodology to form the cell culture and detection zones, respectively. LNCaP cells were seeded directly on the paper substrate and grown in the paper-integrated analytical device. The cell viability and H2S production of LNCaP cells were assessed using a simple water-soluble tetrazolium salt colorimetric assay and H2S-sensing paper, respectively. The H2S-sensing paper showed good sensitivity (sensitivity: 6.12 blue channel intensity/μM H2S, R2 = 0.994) and a limit of quantification of 1.08 μM. As a result, we successfully measured changes in endogenous H2S production in 3D-cultured, live LNCaP cells within the paper-integrated analytical device while varying the duration of incubation and substrate concentration (L-cysteine). This paper-integrated analytical device can provide a simple and effective method to investigate H2S signaling pathways and drug screening in a 3D culture model.

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“…The vast majority of current biochemical assays relies on colorimetric detections, particularly enzyme-linked immunosorbent assay (ELISA) produces a colored solution in the presence of the analyte [4]. Based on the principle that the solution absorbance has a direct correlation with the analyte concentration [5], many standard ELISA protocols have been established and widely utilized in biomedical analysis for decades [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

A WiFi scanner in conjunction with disposable multiplex paper assay for the quantitation of disease markers in blood plasma

et al. 2021

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Herein we report a quantitative, multiplex assay for disease markers in plasma based on an integrated setup of a portable scanner and a disposable paper-based analytical device (PAD). The quantitative analysis relies on the digital colorimetric reading of the three-layer PAD with 30 assay sites for performing respective chromogenic reactions for plasma uric acid, glucose, and triglyceride, which are considered as important risk factors for cardiovascular diseases. A portable scanner with WiFi transmission capability was used to produce high-quality color images of the PADs and wirelessly transfer them to a smartphone or other mobile devices for data processing. The concentrations of biomarkers in both standard solutions and plasma samples can be directly obtained using a custom-designed smartphone app that is also capable of constructing calibration curves. The detection limits of uric acid, glucose, and triglyceride were determined to be 0.50 mg/dL, 0.84 mmol/L, and 14 mg/dL, respectively, which are below the normal limits and adequate for clinical validation. Owing to the distinct advantages-simple, portable, and costeffective-this mobile assay protocol can be used for point-of-care (POC) settings or resource-limited situations, and potentially for the diagnosis and prevention of infectious diseases.

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A simple and facile paper-based colorimetric assay for detection of free hydrogen sulfide in prostate cancer cells

Cited by 42 publications

References 29 publications

Analytical Methods for Detection of Gasotransmitter Hydrogen Sulfide Released from Live Cells

Analytical Methods for Detection of Gasotransmitter Hydrogen Sulfide Released from Live Cells

In Situ Detection of Hydrogen Sulfide in 3D-Cultured, Live Prostate Cancer Cells Using a Paper-Integrated Analytical Device

A WiFi scanner in conjunction with disposable multiplex paper assay for the quantitation of disease markers in blood plasma

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