A simple and established method of tissue culture of human gingival fibroblasts for gingival augmentation.

Saczko, Jolanta; Dominiak, Marzena; Kulbacka, Julita; Chwiłkowska, Agnieszka; Krawczykowska, Honorata

doi:10.2478/v10042-008-0017-4

Cited by 58 publications

(55 citation statements)

References 5 publications

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“…Primary human umbilical vein endothelial cells (HUVEC) were isolated from umbilical cord as described 14 and maintained in medium 199 (GIBCO BRL Life Technologies, Grand, Island, NY) supplemented with 20% FBS, 30 µg/mL endothelial cell growth supplement (Upstate Biotechnology, Lake Placid, NY), 15 µg/ mL heparin (Leo Pharmaceutical Product, Ballerup, Denmark), and 1 mM pyruvate in 5% CO 2 . Human gingival fibroblast (GF) cells were isolated from gingival tissue as described 15 and cultured in DMEM containing 10% FBS in 5% CO …”

Section: Cell Culturementioning

confidence: 99%

Fermentation Product of Soybean, Black Bean, and Green Bean Mixture Induces Apoptosis in a Wide Variety of Cancer Cells

Chia

et al. 2012

Integr Cancer Ther

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Previous studies have shown that soybean fermentation products can act as cancer chemoprevention or therapeutic agents. In this study, the anticancer activities of a fermentation product of soybean, black bean, and green bean mixture (BN999) were investigated. We found that BN999 inhibited the growth of human breast cancer AU565 cells and prostate adenocarcinoma PC-3 cells but not that of normal human cells. BN999 induced apoptosis in various human cancer cells but not in normal human cells. BN999 treatment of AU565 cancer cells resulted in activation of calpain and caspase-8, -9, and -3, suggesting that BN999 induces apoptosis via receptor-, mitochondria-, and endoplasmic reticulum-mediated pathways. Finally, we showed that BN999 inhibited the growth of mouse CT-26 colon cancer xenografts in syngenic BALB/c mice without causing obvious side effects. Together, these data suggest that BN999 has potential to be used as a cancer chemoprevention or therapeutic agent.

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Section: Cell Culturementioning

confidence: 99%

Fermentation Product of Soybean, Black Bean, and Green Bean Mixture Induces Apoptosis in a Wide Variety of Cancer Cells

Chia

et al. 2012

Integr Cancer Ther

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“…Fibroblasts were isolated from soft tissue of gingival. The cells were isolated from the healthy gingival tissues according to the procedure described by Saczko et al [17] . Both cell lines were harvested in Dulbecco medium (Sigma) with 10% of FBS and addition of antibiotics.…”

Section: Cell Culturementioning

confidence: 99%

ETM study of electroporation influence on cell morphology in human malignant melanoma and human primary gingival fibroblast cells

Skołucka

Daczewska

Saczko

et al. 2011

Asian Pacific Journal of Tropical Biomedicine

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“…Recent technological advances in tissue engineering suggest its application in several medical fields, including periodontology (1). The cell culture technique allows the study of the biological behavior of cells, considering the kinetics of cell proliferation and the biosynthesis of many components of the extracellular matrix (2).…”

Section: Introductionmentioning

confidence: 99%

Morphological, Functional and Biochemical Characterization of Canine Gingival Fibroblasts

et al. 2013

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As dogs are good models for in vivo studies, it is interesting to evaluate the behavior of canine gingival fibroblasts (CGF) in vitro, so that these cells could be seeded on a matrix and later studied in vivo. The aim of this study was to perform a morphological, functional and biochemical analysis of CGF, comparing it with human gingival fibroblasts (HGF), as well as to evaluate the change of their characteristics over several passages. Using gingival fibroblasts from 3 dogs and 3 humans in the subculture (Sub), first (P1), third (P3), fifth (P5) and seventh (P7) passages, the following parameters were assessed: cell morphology, spreading, adhesion, viability and total protein content. The results showed no major differences between the passages in terms of morphology and spreading, and a tendency of greater adhesion and viability for HGF when compared with CGF. The total protein content was significantly higher for HGF. HGF exhibited greater functional and biochemical activity in vitro compared to CGF. Higher numbers at Sub were observed for both CGF and HGF in all evaluated parameters. The differences do not prevent the use of CGF for tissue engineering, but its use seems to be more appropriate in the subculture or first passage.

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A simple and established method of tissue culture of human gingival fibroblasts for gingival augmentation.

Cited by 58 publications

References 5 publications

Fermentation Product of Soybean, Black Bean, and Green Bean Mixture Induces Apoptosis in a Wide Variety of Cancer Cells

Fermentation Product of Soybean, Black Bean, and Green Bean Mixture Induces Apoptosis in a Wide Variety of Cancer Cells

ETM study of electroporation influence on cell morphology in human malignant melanoma and human primary gingival fibroblast cells

Morphological, Functional and Biochemical Characterization of Canine Gingival Fibroblasts

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