A simple and efficient method for cultivation of limbal explant stem cells with clinically safe potential

Çan, Gamze; Akdere, Özge Ekin; Can, Mehmet Erol; Gümüşderelı́oğlu, Menemşe

doi:10.1016/j.jcyt.2018.11.005

Cited by 5 publications

(3 citation statements)

References 42 publications

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“…Platelet rich fibrin (PRF), a completely autologous substance obtained by centrifugation of peripheral venous blood, contains platelets, leukocytes and several biologically active proteins including platelet alpha granules, platelet−derived growth factor (PGDF), transforming growth factors−β (TGF−β), vascular endothelial growth factors (VEGF), and epidermal growth factors ( 32 , 33 ) in a fibrin matrix. PRF has been suggested to have beneficial effects on viability of tumor explants ( 32 , 34 ).…”

Section: Introductionmentioning

confidence: 99%

Optimizing culturing conditions in patient derived 3D primary slice cultures of head and neck cancer

et al. 2023

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BackgroundThree-dimensional primary slice cultures (SC) of head and neck squamous cell carcinomas (HNC) are realistic preclinical models. Until now, preserving structure and viability ex vivo for several days has been difficult. The aim of this study was to optimize cultivation conditions for HNC SC and analyze the added effects of platelet rich fibrin (PRF) on these conditions.MethodsSC were prepared from the tumor biopsies of 9 HNC patients. Cultures were incubated for 1 and 7 days in three different media- Keratinocyte serum-free medium (SFM), RPMI-1640i, and 1:1 mix of both, with and without addition of PRF. After culturing, SC were fixated, embedded, and stained with Hematoxylin-Eosin (HE) and cleaved caspase-3. In addition, triple immune fluorescence staining for cytokeratin, vimentin and CD45 was performed. Outcome parameters were cell count and cell density, viability and apoptosis, SC total area and proportions of keratinocytes, mesenchymal and immune cells. The effects of culture time, medium, and addition of PRF were calculated in an SPSS generalized linear model and using the Wald Chi-Squared test.ResultsNinety-four slice cultures were analyzed. Viability remained stable for 7 days in culture. After addition of PRF, cell viability increased (p=0.05). SC total area decreased (0.44 ± 0.04 mm2 on day 1 (95% CI: 0.35 to 0.56) to 0.29 ± 0.03 mm2 on day 7 (95% CI: 0.22 to 0.36), but cell density and cell proportions remained stable. Differences in cultivation media had no significant impact on outcome parameters.ConclusionHNC SC can be preserved for up to 7 days using the tested cultivation media. Cell viability was best preserved with addition of PRF. HNC SC are a versatile experimental tool to study physiology and drug actions. Autologous PRF can help simulate realistic conditions in vitro.

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Section: Introductionmentioning

confidence: 99%

Optimizing culturing conditions in patient derived 3D primary slice cultures of head and neck cancer

et al. 2023

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“…Platelet‐rich fibrin (PRF) is a second generation of autologous platelet concentrate, which predominantly consists of platelets, white blood cells, and fibrin. It contains molecules, such as growth factors and cytokines 7 that play important roles in wound healing, and have been widely used in other medical fields 8,9 . PRF activates platelet‐derived growth factor (PDGF), vascular endothelial growth factor (VEGF), insulin‐like growth factor (IGF), and so on, that are highly correlated with endometrial repair.…”

Section: Introductionmentioning

confidence: 99%

“…It contains molecules, such as growth factors and cytokines 7 that play important roles in wound healing, and have been widely used in other medical fields. 8,9 PRF activates platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), insulinlike growth factor (IGF), and so on, that are highly correlated with endometrial repair. However, clinical applications to promote the growth of the endometrium and improve pregnancy rate are still lacking.…”

Section: Introductionmentioning

confidence: 99%

Efficacy and safety of autologous platelet‐rich fibrin for the treatment of infertility with intrauterine adhesions

Wang

Yang

Mao

et al. 2021

J of Obstet and Gynaecol

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Aim To evaluate the efficacy and safety of autologous platelet‐rich fibrin (PRF) for the treatment of infertility with intrauterine adhesions. Methods Forty patients who suffered from infertility with intrauterine adhesions (IUAs) were enrolled in this prospective trial from October 2018 to December 2019. They were randomly divided into a PRF group and a control group. Both groups underwent transcervical resection of adhesions (TCRA) during the follicular phase. PRF group: PRF prepared from autologous venous blood was placed in the uterine cavity after TCRA and after the first menstrual re‐fluid; control group: no PRF insertion. After the second menstrual re‐fluid, both groups underwent hysteroscopy to reevaluate adhesion severity. Pregnancy rate, intrauterine adhesion score, menstrual volume and duration, and endometrial thickness were assessed and adverse events were recorded. Results (1) PRF group pregnancy rate was significantly higher than the control group (55.0% vs. 20.0%). Mean follow‐up time was (17.63 ± 2.33) months. All patients fell pregnant naturally. Four (PRF) and one (control) patients delivered live births without placental complications. (2) Intrauterine adhesion scores decreased compared with treatment‐before. (3) In the pictorial blood loss assessment chart, score and menstrual duration during the 3rd menstrual cycle showed significant improvements for both groups. (4) Endometrial thickness also showed an increasing trend, and subendometrial bloodflow improved. (5) No treatment‐related serious adverse events were noted. Conclusion Placing PRF into the uterine cavity of infertility patients with IUAs following TCRA is a safe and effective therapeutic method.

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Evaluation of the cytotoxic and genotoxic/antigenotoxic effects of resveratrol in human limbal explant cultures

2022

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A simple and efficient method for cultivation of limbal explant stem cells with clinically safe potential

Cited by 5 publications

References 42 publications

Optimizing culturing conditions in patient derived 3D primary slice cultures of head and neck cancer

Optimizing culturing conditions in patient derived 3D primary slice cultures of head and neck cancer

Efficacy and safety of autologous platelet‐rich fibrin for the treatment of infertility with intrauterine adhesions

Evaluation of the cytotoxic and genotoxic/antigenotoxic effects of resveratrol in human limbal explant cultures

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