A simple and efficient method for betalain quantification in RUBY-expressing plant samples
Dibyajyoti Pramanik,
Keunsub Lee,
Kan Wang
Abstract:The RUBY reporter system has demonstrated great potential as a visible marker to monitor gene expression in both transiently and stably transformed plant tissues. Ectopic expression of the RUBY reporter leads to bright red pigmentation in plant tissues that do not naturally accumulate betalain. Unlike traditional visual markers such as β-glucuronidase (GUS), luciferase (LUC), and various fluorescent proteins, the RUBY reporter system does not require sample sacrifice or special equipment for visualizing the ge… Show more
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