A Simple and Easy Method of Monitoring Doxorubicin Release from a Liposomal Drug Formulation in the Serum Using Fluorescence Spectroscopy

Abstract

Formulation of a drug as liposomes facilitates its delivery to the disease target. Rightly, liposomes are gaining popularity in the medical field. In order for the drug to show efficacy, release of the encapsulated drug from the liposome at the target site is required. However, the release is affected by the permeability of the lipid bilayer of the liposome, and it is important to examine the effect of the surrounding environment on the permeability. In this study, we showed the usefulness of fluorescence analysis, especially fluorescence fingerprint, for a rapid and simple monitoring of release of an encapsulated anticancer drug (doxorubicin) from its liposomal formulation (DOXIL). Our result indicated that the release is accelerated by the existence of membrane permeable ions, such as tris(hydroxymethyl)aminomethane, and blood proteins like albumin. Hence, monitoring of doxorubicin release by fluorescence analysis is useful for the efficacy evaluation of DOXIL in a biomimetic environment.

Surface Modification of Mesoporous Silica Nanoparticles for Application in Targeted Delivery Systems of Antitumour Drugs

“…One of the possible ways of reducing the toxicity of doxorubicin is the use of nanoscale transport systems for its transfer. Doxorubicin has intrinsic fluorescence in the red region of the spectrum (λ exc /λ em 495/595 nm), so it is a convenient target for studying drug transport processes, including their intracellular penetration and release [ 166 ]. DOX is actively used as a model drug for evaluating drug loading and delivery using a variety of nanomaterials, including MSNs ( Table 2 ).…”

Adapting Clofazimine for Treatment of Cutaneous Tuberculosis by Using Self-Double-Emulsifying Drug Delivery Systems

“…Encapsulation of the lipophilic drugs into inert lipid vehicles is shown to provide reproducible drug concentration profiles [ 173 , 177 ]. Lipid carrier systems include oils, surfactant dispersions, emulsions, nanoemulsions, liposomes, solid lipid carrier systems, nanostructured lipid carrier systems, self-emulsifying drug delivery systems (SEDDSs) and SDEDDSs [ 173 , 177 , 178 , 179 ]. Overall, lipid carrier systems can facilitate targeted drug delivery, thereby improving therapeutic effects and decreasing side effects, which will be important for anti-tubercular treatment [ 178 ].…”

“…Liposomes, emulsions and nanoemulsions are frequently employed as drug delivery systems. These lipid-based formulations are also attracting increasing attention for enhancing topical and transdermal drug delivery [ 178 , 179 , 180 , 181 ]. Liposomes are spherical, bilayer structures of 100–200 nm in diameter.…”

“…They are not as widely utilized for topical or transdermal drug delivery and are mainly included in cosmetics and long-acting sunscreen formulations, although their membrane structure is similar to that of natural membranes found in skin. Liposomes are expected to remain at the surface of the skin where they fuse with the skin lipids and slowly release the encapsulated drug [ 179 , 182 , 183 , 184 ].…”

Simple and Rapid Evaluation of the Unique Manuka Factor in Manuka Honey Using Fluorescence Fingerprints and Principal Component Analysis

“…15,16) Combining fluorescent fingerprint with principal component analysis (PCA) has proven useful in the distinction of honey production areas. 17,18) Since fluorescence fingerprint is effective for the analysis of the components and production areas of honey, we expected that UMF, which is an indicator of antibacterial activity specific to manuka honey, could also be evaluated by fluorescence fingerprint, however, no studies have reported the relationship between UMF and the fluorescence fingerprint. Hence, this study aimed to determine the UMF of manuka honey samples by analyzing fluorescence fingerprint data using PCA and partial least squares (PLS) regression.…”