A Simple and Cost-Effective TLC-Densitometric Method for the Quantitative Determination of Acetylsalicylic Acid and Ascorbic Acid in Combined Effervescent Tablets

Abstract

A new, simple, and cost-effective TLC-densitometric method has been established for the simultaneous quantitative determination of acetylsalicylic acid and ascorbic acid in combined effervescent tablets. Separation was performed on aluminum silica gel 60F254 plates using chloroform-ethanol-glacial acid at a volume ratio of 5:4:0.03 as the mobile phase. UV densitometry was performed in absorbance mode at 200 nm and 268 nm for acetylsalicylic acid and ascorbic acid, respectively. The presented method was validated as per ICH guidelines by specificity, linearity, accuracy, precision, limit of detection, limit of quantification, and robustness. Method validations indicate a good sensitivity with a low value of LOD and LOQ of both examined active substances. The linearity range was found to be 1.50–9.00 μg/spot and 1.50–13.50 μg/spot for acetylsalicylic and ascorbic acid, respectively. A coefficient of variation that was less than 3% confirms the satisfactory accuracy and precision of the proposed method. The results of the assay of combined tablet formulation equal 97.1% and 101.6% in relation to the label claim that acetylsalicylic acid and ascorbic acid fulfill pharmacopoeial requirements. The developed TLC-densitometric method can be suitable for the routine simultaneous analysis of acetylsalicylic acid and ascorbic acid in combined pharmaceutical formulations. The proposed TLC-densitometry may be an alternative method to the modern high-performance liquid chromatography in the quality control of above-mentioned substances, and it can be applied when HPLC or GC is not affordable in the laboratory.

Use of TLC-Densitometric Method for Determination of Valproic Acid in Capsules

“…Limit of detection and quantification was determined by preparing three standard solutions of valproic acid at concentration of 15, 10, and 7.5 mg/5 mL (in the case of valproic acid detected with 2% CuSO 4 solution), and three standard solutions of valproic acid at concentration of 1.0, 0.9, and 0.8 mg/5 mL (in the case of valproic acid detected by the 2 ,7 -dichlorofluorescein-aluminum chloride-iron [III] chloride system). The LOD and LOQ calculation methodology was given in our earlier works [47][48][49]. The analyses were repeated three times.…”

A new approach for studying the stability and degradation products of ascorbic acid in solutions

“…Other analytical techniques intensively employed for studying the stability of ascorbic acid are polarography [13,14], voltametric methods [15] spectrometric UV-VIS methods [16], capillary electrophoresis (CE) [17], thin layer chromatography (TLC) [18] gas chromatography (GC) and HPLC methods [19].…”

Green-synthesized selenium nanoparticles using garlic extract and their application for rapid detection of salicylic acid in milk

“…There is a lot of toxicants added in milk as adulteration by using nanotechnology the recent research is emphasizing on, salicylic acid (C 7 H 6 O) which is known to be added in milk as a preservative in order to enhance shelf life and is associated with gastric irritation, bleeding, diarrhea, renal disease and even death in mammals (Nagraik et al, 2021). Traditional detection methods of salicylic acid (SA) include high-end advanced instruments that are LCMS (Liquid Chromatography-Mass Spectrometry) (Protasiuk & Olejnik, 2018), Gas Chromatography-Mass Spectroscopy (Božić Luburić et al, 2022) TLC, and High-Performance Liquid Chromatography (Pyka-Pająk et al, 2018).…”