A simple and cost-effective protocol for DNA isolation from buccal epithelial cells

Aidar, Marisi; Line, Sérgio Roberto Peres

doi:10.1590/s0103-64402007000200012

Cited by 218 publications

(162 citation statements)

References 15 publications

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“…Genomic DNA of all individuals was isolated from buccal epithelial cells by the proteinase K digestion followed by ammonium acetate extraction method [Aidar and Line, 2007]. The SNPs used in this study were chosen from previous studies where associations with oral clefts [Riley et al, 2007] and breast cancer [Easton et al, 2007;Hunter et al, 2007] were described.…”

mentioning

confidence: 99%

Studies of genes in the FGF signaling pathway and oral clefts with or without dental anomalies

Menezes

Letra

Ruff

et al. 2008

American J of Med Genetics Pt A

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mentioning

confidence: 99%

Studies of genes in the FGF signaling pathway and oral clefts with or without dental anomalies

Menezes

Letra

Ruff

et al. 2008

American J of Med Genetics Pt A

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“…Summary of results from protocols tested in this study. Short and straightforward methods are preferred for bacterial community analyses (Aidar and Line, 2007;Hamady and Knight, 2009). Protocols 4, 5 and 6 were suitable methods, as they were relatively easy and quick (6, 5 and 4 h, respectively).…”

Section: Resultsmentioning

confidence: 99%

Methodology Simple and inexpensive DNA extraction protocol for studying the bacterial composition of sludges used in microbial fuel cells

Canto-Canché¹,

Tzec-Simá²,

Vázquez-Loría³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Bacteria oxidize organic matter and nutrients to produce electric energy in microbial fuel cells (MFC) -a technology of increasing importance because of its sustainability. To improve the performance of MFCs, it is necessary not only to gain a better understanding of MFC engineering designs, but also to improve the understanding of the composition of the microbial communities in MFCs. Fast and efficient DNA extraction protocols that are suitable for extracting diverse bacterial genomes are necessary to identify the bacterial diversity present in MFCs and to further monitor the dynamic changes of microbial communities. This study focused on testing different direct cell lysis protocols to extract DNA from a microbial sludge harvested DNA extraction of bacterial consortium in MFC from an MFC. The protocol that achieved the best results was based on a previous study, but was modified by eliminating a chaotropic salt and the special columns used for nucleic acid purification. The efficiency of this less expensive and more straightforward protocol was confirmed by PCR amplification of the 16S rRNA gene and denaturing gradient gel electrophoresis analysis, which confirmed the extraction of multiple genomes. The sequences of 10 clones revealed the presence of phyla, Proteobacteria, Firmicutes and Actinobacteria, comprising both Gramnegative and Gram-positive bacteria. Some of these bacteria were identified at the genus level, e.g., Clostridium, Pseudoxanthomonas, Tistrella, and Enterobacter; these genera have been described in active sludges from wastewater treatment, supporting the congruency of our results. Therefore, this protocol is a useful tool for analysis of the bacteria responsible for energy production in MFCs.

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“…The three protocols proposed here included two in-house procedures of low cost (Aidar, 2007) and low-toxicity as it does not use phenol-chloroform extraction (Lum, 1998;Rogers, 2007).…”

Section: Dna Yield and Puritymentioning

confidence: 99%

The Use of DNA Extraction for Molecular Biology and Biotechnology Training: A Practical and Alternative Approach

Lázaro-Silva¹,

Mattos²,

Castro³

et al. 2015

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DNA extraction and polymerase chain reaction are molecular biology techniques widely used in research, diagnostics and biotechnology industry. In this work, we report a practical course designed for undergraduate students of health, biological sciences and biotechnology areas. The course is five days long and emphasizes basic procedures in practical activities. The students were challenged to use three different protocols for DNA isolation (one using commercial silica column and two, in-house) using no lab animals but themselves. They evaluated the DNA yield, and potential amplification of the extracted DNA by PCR using their own cells genetic material. Students were stimulated to adopt a critical view during the course to develop skills including critical analysis of primary results and possible errors, scientific report writing and a problem solving profile.

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A simple and cost-effective protocol for DNA isolation from buccal epithelial cells

Cited by 218 publications

References 15 publications

Studies of genes in the FGF signaling pathway and oral clefts with or without dental anomalies

Studies of genes in the FGF signaling pathway and oral clefts with or without dental anomalies

Methodology Simple and inexpensive DNA extraction protocol for studying the bacterial composition of sludges used in microbial fuel cells

The Use of DNA Extraction for Molecular Biology and Biotechnology Training: A Practical and Alternative Approach

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