A Simple 3-D Microelectrode Fabrication Process and Its Application in Microfluidic Impedance Cytometry

Shen, Hailong; Ji, Bowen; Feng, Huicheng

doi:10.1109/jsen.2022.3211443

Cited by 6 publications

(2 citation statements)

References 47 publications

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“…(vi) The solidified PDMS and the glass with ITO electrodes were plasma cleaned and well bonded using a plasma machine (PDC-MG, PTL Technology Co, Ltd., Shenzhen China). Finally, the bonded MIC chip was placed on the heating plate for 30 min at 80 °C) [ 32 ]. The schematic diagram of the bonded MIC chip is shown in Figure 1 c.…”

Section: Methodsmentioning

confidence: 99%

Label-Free Sensing of Cell Viability Using a Low-Cost Impedance Cytometry Device

et al. 2023

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Cell viability is an essential physiological status for drug screening. While cell staining is a conventional cell viability analysis method, dye staining is usually cytotoxic. Alternatively, impedance cytometry provides a straightforward and label-free sensing approach for the assessment of cell viability. A key element of impedance cytometry is its sensing electrodes. Most state-of-the-art electrodes are made of expensive metals, microfabricated by lithography, with a typical size of ten microns. In this work, we proposed a low-cost microfluidic impedance cytometry device with 100-micron wide indium tin oxide (ITO) electrodes to achieve a comparable performance to the 10-micron wide Au electrodes. The effectiveness was experimentally verified as 7 μm beads can be distinguished from 10 μm beads. To the best of our knowledge, this is the lowest geometry ratio of the target to the sensing unit in the impedance cytometry technology. Furthermore, a cell viability test was performed on MCF-7 cells. The proposed double differential impedance cytometry device has successfully differentiated the living and dead MCF-7 cells with a throughput of ~1000 cells/s. The label-free and low-cost, high-throughput impedance cytometry could benefit drug screening, fundamental biological research and other biomedical applications.

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Section: Methodsmentioning

confidence: 99%

Label-Free Sensing of Cell Viability Using a Low-Cost Impedance Cytometry Device

et al. 2023

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“…40 Three-dimensional electrodes can generate a more uniform current density, which can promote the sensitivity and throughput of microfluidic impedance cytometers in the detection of cells. 40,41 To date, two types of three-dimensional electrodes have been developed, including facing electrode [43][44][45] and special three-dimensional electrode 46,47 configurations. Because facing electrodes can't thoroughly address the issues of nonuniform electric field and complicated fabrication, microfluidic impedance cytometers with such electrodes may not achieve the detection of microalgal cells in a sensitive and low-cost manner.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic impedance cytometry with flat-end cylindrical electrodes for accurate and fast analysis of marine microalgae

Chen,

Shen,

Liu

et al. 2024

Lab Chip

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A microfluidic impedance cytometry device for robust identification of H. pluvialis

Shen,

Chen,

et al. 2024

Anal. Methods

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A Simple 3-D Microelectrode Fabrication Process and Its Application in Microfluidic Impedance Cytometry

Cited by 6 publications

References 47 publications

Label-Free Sensing of Cell Viability Using a Low-Cost Impedance Cytometry Device

Label-Free Sensing of Cell Viability Using a Low-Cost Impedance Cytometry Device

Microfluidic impedance cytometry with flat-end cylindrical electrodes for accurate and fast analysis of marine microalgae

A microfluidic impedance cytometry device for robust identification of H. pluvialis

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