A silver metal complex as a luminescent probe for enzymatic sensing of glucose in blood plasma and urine

Abstract

In this work, we present a facile preparation of a paper-based glucose assay for rapid, sensitive, and quantitative measurement of glucose in blood plasma and urine. Two copper phosphorescent complexes [Cu(2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline)(2,6-dimethylphenylisocyanide)2][B(C6H3(CF3)2)4] (Cu1) and [Cu(2,9-dimethyl-1,10-phenanthroline)(2,6-dimethylphenylisocyanide)2][B(C6H3(CF3)2)4] (Cu2) and a new silver congener [Ag(P3)CNAg(P3)][B(C6H3(CF3)2)4] (Ag3) (P3 = PPh2C6H4-PPh-C6H4PPh2 [bis(o-diphenylphosphinophenyl)phenylphosphine]) have been synthesized and their oxygen sensing abilities were investigated. The dimetallic phosphine-based Ag3 complex, having a high oxygen sensing ability, was employed as an efficient signal transducer in enzymatic reactions to recognize blood plasma glucose and urine glucose, which provided a wide linear response for a concentration range between 1.0 and 35 mM and a rapid response, with a limit of detection (LOD) of 0.09 mM for glucose. In practical application, this Ag3 paper-based device offers great analytical reliability and accuracy upon monitoring glucose concentrations in blood plasma.

Paper-based microfluidic devices based on 3D network polymer hydrogel for the determination of glucose in human whole blood

“…The analytical performance of the analytical device was also compared with those of other reported luminescent glucose sensing devices using gel capping (Table 1). [27][28][29][30][31][32][33][34] As shown in Table 1, the proposed method provides detection sensitivity for glucose comparable with those of some reported methods and could meet the requirement for glucose detection in clinical glucose monitoring and the food industry (mM levels).…”

“…The oxygen collision efficiency measurement procedures were similar to those in our previous report. 27 10 mL of Cu4c (1 mM) in DCM was added to the lter paper, and the lms were used for oxygen collision efficiency measurement. The prepared lms were then placed into the Linkam FTIR600 stage, and N 2 (99.999%) and O 2 (99.999%) were mixed at different concentrations via mass ow controllers and passed to a Linkam FTIR600 stage.…”

Detecting Glucose Levels in Blood Plasma and Artificial Tear by Au(I) Complex on the Carbopol Polymer: A Microfluidic Paper-Based Method

“…Furthermore, upon excitation of B5 at higher lying electronic states, the RT phosphorescence can be significantly enhanced due to the increased population in the T 1 state via faster rate of S i → T j ( I > 1, j ≥ 1) intersystem crossing (cf. S 1 → T j ( j ≥ 1) [9]. Despite these intriguing and unique photophysical properties, however, exploiting B5 and its numerous congeners in solid state applications such as sensing are scarce [9,10].…”

“…S 1 → T j ( j ≥ 1) [9]. Despite these intriguing and unique photophysical properties, however, exploiting B5 and its numerous congeners in solid state applications such as sensing are scarce [9,10].…”

“…The results elaborated above inspired us to develop a facile methodology to prepare complex B5 on a μPAD, which can be employed selectively to measure plasma glucose levels in human blood and tear glucose. The detection principle of plasma glucose has been well found in ref [9] and works on the increase of emission intensity of phosphorescence due to the consumption in oxygen concentration via oxidation reaction of glucose by glucose oxidase (GOx); also see Equation (1). In this study we also developed a wax-printed device for measuring tear glucose.…”