“…strain YL cell culture in a medium containing 2-chloropropionate (2-CPA) as the sole carbon source [5]. The reaction mechanism of l-DEX YL has been analyzed by site-directed mutagenesis [6], X-ray crystallography [7][8][9], mass spectrometry [10][11][12], 18 O-incorporation experiments [13], and computer simulations with molecular dynamics (MD), fragment molecular orbital (FMO) [14] and quantum-mechanical/molecularmechanical (QM/MM) methods [15]. Site-directed mutagenesis experiments have revealed that some amino acid residues are essential for the catalytic function of l-DEX YL [6].…”