A Significant Fluorescent Aptamer Sensor Based on Carbon Dots and Graphene Oxide for Highly Selective Detection of Progesterone

Abstract

In this paper, a uorescent aptamer sensor was constructed based on the carbon dots and graphene oxide. This sensor combines the excellent uorescence performance of carbon dots with the high speci city of aptamers to detect progesterone with high sensitivity and selectivity. In the absence of progesterone, the carbon dots-aptamer system and graphene oxide form a uorescence resonance energy transfer process(FRET), which quenches the uorescence of the carbon dots. When progesterone is added, the aptamer speci cally binds to progesterone, resulting the uorescence of the carbon dots is recovered. At optimal conditions, the uorescence intensity recovered by the carbon dots has a linear relationship with the concentration of progesterone in the range of 0.1-120 nM, and the detection limit is 3.3×10 -11 M. Besides, the sensor has satisfactory detection results of progesterone in milk, indicating that this method has a enormous potential for application in food safety.

Functional nanostructures in analytical chemistry: new insights into the optical and electrochemical sensing of animal hormones in food, environmental and biological samples

“…A fluorescence approach was developed for progesterone assay using GO-CDs as a probe. 89 A conjugate of progesterone and CDs with graphene oxide formed an aptamer-based sensor for the detection of hormone. The as-prepared CDs showed the highest excitation at 355 nm with an intense emission at 450 nm.…”

Advances in the ratiometric combination of quantum dots for their use in sensing applications

“…In a recent work by Cui et al , CQDs were used with graphene oxide (GO) as a FRET-based sensor for the detection of progesterone. 142 Moreover, research is being conducted to bring forth new methodologies for detection systems, such as the use of a Scanning Kelvin Probe microscope (SKP) for the detection of volatile organic compounds (VOC). In the work by Selvaraju et al , 143 the work function and interactions of ethanol gas with the carbon quantum dots were investigated using SKP.…”

Carbon Dots Conjugated Antibody as an Effective FRET-Based Biosensor for Progesterone Hormone Screening

“…However, the detection of FRET-based progesterone hormone using a donor-acceptor combination of CD and GO is in the early stages of investigation. Recently, a significant and sensitive FRET aptasensor for progesterone based on carbon dots has been reported by H. Cui et al with a reduced detection limit 3.3 × 10 −11 M [ 31 ]. However, the detection range is limited to 120 nM in milk, which is not suitable for aqueous samples where progesterone is also present at higher levels, i.e., >120 nM [ 32 ].…”