A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity

Xu, Xiaowei; Zhang, Fan; Liang, Chaoyang; Li, Ling; Wang, Lili; Liang, Yingchun; Wu, Jun; Chang, Shaohong; Zhang, Yan; Lv, Zhaohui; Fu, Jing; Liu, Yang; Jin, Shuai; Wang, Tao; Tian, Hong; Dong, Yishan; Ding, Lei; Cheng, Long; Li, Rui; Fu, Shihua; Jiao, Shunchang; Ye, Qinong

doi:10.1038/ncomms14059

Cited by 51 publications

(51 citation statements)

References 51 publications

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“…Because mitotic catastrophe is an important mechanism of cell death in solid tumors receiving clinical radiotherapy, we next assessed mitotic catastrophe after a 4‐Gy dose of XRT or PRT in our 4 HNSCC cell lines by co‐staining the cytoplasm for γ‐tubulin (with Texas Red) and the nucleus with DAPI (blue) and noting morphologic changes in the nuclei . The percentages of cells in mitotic catastrophe in the control conditions ranged from 11.45% (±4.55) to 29.54% (±2.46).…”

Section: Resultsmentioning

confidence: 99%

“…Immunoreactions were visualized with a Leica Microsystem (Wetzlar, Germany) at ×100 magnification, and cells were photographed in at least 10 randomly chosen fields. Cells with multinucleated enlarged nuclei, complete aberrant nuclei, prematurely condensed chromosomes without cytoplasm shrinkage and nuclear fragmentation, multiple micronuclei, catastrophic clusters, and nuclei undergoing multinucleation were considered to be in mitotic catastrophe . Those cells in mitotic catastrophe were counted as a percentage of all cells, and the results are shown as the average percentages from 3 independent experiments.…”

Section: Methodsmentioning

confidence: 99%

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Proton versus photon radiation–induced cell death in head and neck cancer cells

et al. 2018

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Background: Photon (X-ray) radiotherapy (XRT) kills cells via DNA damage, however, how proton radiotherapy (PRT) causes cell death in head and neck squamous cell carcinoma (HNSCC) is unclear. We investigated mechanisms of HNSCC cell death after XRT versus PRT. Methods: We assessed type of death in 2 human papillomavirus (HPV)-positive and two HPV-negative cell lines: necrosis and apoptosis (Annexin-V fluorescein isothiocyanate [FITC]); senescence (β-galactosidase); and mitotic catastrophe (γ-tubulin and diamidino-phenylindole [DAPI]). Results: The XRT-induced or PRT-induced cellular senescence and mitotic catastrophe in all cell lines studied suggested that PRT caused cell death to a greater extent than XRT. After PRT, mitotic catastrophe peaked in HPV-negative and HPV-positive cells at 48 and 72 hours, respectively. No obvious differences were noted in the extent of cell necrosis or apoptosis after XRT versus PRT. Conclusion: Under the conditions and in the cell lines reported here, mitotic catastrophe and senescence were the major types of cell death induced by XRT and PRT, and PRT may be more effective. K E Y W O R D Scell death mechanisms, head and neck cancer, photon radiotherapy, proton radiotherapy, radiation-induced cell death

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Proton versus photon radiation–induced cell death in head and neck cancer cells

et al. 2018

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“…At the different time points, the two rats were sacrificed and full‐thickness skin samples including intact skin adjacent to the wound, the wound margin and epithelialized wounds were collected and prepared as tissue slices. Slices were then examined using HE (hematoxylin and eosin) and Sirius Red staining …”

Section: Methodsmentioning

confidence: 99%

Effect of the hyaluronic acid‐poloxamer hydrogel on skin‐wound healing: in vitro and in vivo studies

Li²,

Feng

et al. 2019

Anim Models and Exp Med

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Background Recent research into skin injury and wound healing has focused mainly on post‐trauma hemostasis, infection prevention, dermal regeneration and angiogenesis. However, less attention has been paid to air permeability and moisture loss prevention which also play important roles in injury healing. Methods In the present work, we prepared a hyaluronic acid‐poloxamer ( HA ‐ POL ) hydrogel and tested the therapeutic effect of the hydrogel on skin‐wound healing. Results The HA ‐ POL hydrogel transformed from sol to gel at 30°C, close to body temperature, and had stable moisturizing properties. HA ‐ POL hydrogel promoted skin‐wound healing and increased protein accumulation in the wound area. HA ‐ POL hydrogel allowed greater air permeability than Band‐aid, a typical wound covering. Results from transwell assays showed that the HA ‐ POL hydrogel effectively isolated skin‐wounds from bacterial invasion. Conclusion This work demonstrates the advantages of using HA ‐ POL gel materials in the treatment of cutaneous wounds.

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“…Quantitative real-time PCR (qPCR) was performed following the methods described in references. 35,36 The level of β-actin mRNA was measured as a loading control. Primers used in qPCR experiments are listed in Table 1.…”

Section: Quantitative Polymerase Chain Reactionmentioning

confidence: 99%

<p>ARQ-197 enhances the antitumor effect of sorafenib in hepatocellular carcinoma cells via decelerating its intracellular clearance</p>

Gao¹,

Chen²,

Huang³

et al. 2019

OTT

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Background: Hepatocellular carcinoma (HCC) is one of the heaviest malignant burdens in China. Molecular targeting agent, sorafenib, is the main therapeutic option for antitumor therapy of advanced HCC, but it is currently too expensive for the public and its therapeutic effect does not satisfy initial expectation. Therefore, it is important to develop more effective molecular targeted therapeutic strategies for advanced HCC. Materials and methods: The antitumor effects of sorafenib or ARQ-197, an antagonist of c-MET (tyrosine-protein kinase Met or hepatocyte growth factor receptor), were examined by MTT or in murine tumor model. The effect of ARQ-197 on epithelial-mesenchymal transition (EMT) or multidrug resistance (MDR) was examined by quantitative real-time PCR for the expression of related genes. The clearance of sorafenib in HCC cells was detected by liquid chromatography-mass spectrometry/mass spectrometry. Results: ARQ-197 treatment enhanced the sensitivity of HCC cells to sorafenib. Mechanistic studies indicated that ARQ-197 inhibited the expression of EMT-and MDR-related genes. Moreover, ARQ-197 treatment decelerated the clearance of sorafenib in cultured HCC cells and subcutaneous HCC tumors in nude mice. Conclusion: In the present work, our data suggested that ARQ-197 decelerated the clearance of sorafenib in HCC cells and enhanced the antitumor effect of sorafenib.

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A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity

Cited by 51 publications

References 51 publications

Proton versus photon radiation–induced cell death in head and neck cancer cells

Proton versus photon radiation–induced cell death in head and neck cancer cells

Effect of the hyaluronic acid‐poloxamer hydrogel on skin‐wound healing: in vitro and in vivo studies

<p>ARQ-197 enhances the antitumor effect of sorafenib in hepatocellular carcinoma cells via decelerating its intracellular clearance</p>

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