A signal-enhancement fluorescent aptasensor based on the stable dual cross DNA nanostructure for simultaneous detection of OTA and AFB1

Recent Advances in Biological Applications of Aptamer-Based Fluorescent Biosensors

“…Suo et al reported an aptasensor with improved design flexibility by modifying the phosphor and quencher into different ssDNA strands [31]. This aptasensor is a dual crossover DNA nanostructure modified with Cy3 and Cy5 and can simultaneously detect ochratoxin A (OTA) and aflatoxin B1 (AFB1), which are mycotoxins produced by fungi.…”

Target‐mediated suppression of rolling circle amplification for label‐free detection of aflatoxin B1

“…[22][23][24][25] Among them, the fluorescent aptasensors have attracted considerable attention due to their simple operation, high sensitivity, and excellent stability in complexed food matrix. 26 However, a lot of detection methods reported are still exposed to some drawbacks, including the complex chemical label of fluorophores, multistep signal generation step, or long incubation time for sample testing. [27][28][29][30][31][32][33][34][35][36][37][38][39] To circumvent such drawbacks, label-free DNA amplification strategies could be employed for AFB 1 aptasensors, such as hybridization chain reaction (HCR), polymerase chain reaction (PCR), strand displacement amplification (SDA), helicase dependent amplification (HDA) and rolling circle amplification (RCA).…”

“…Taking advantage of aptamers, researchers have designed a variety of aptamer‐based assays for AFB 1 detection, such as fluorescent, colorimetric, or electrochemical aptasensors 22–25 . Among them, the fluorescent aptasensors have attracted considerable attention due to their simple operation, high sensitivity, and excellent stability in complexed food matrix 26 . However, a lot of detection methods reported are still exposed to some drawbacks, including the complex chemical label of fluorophores, multistep signal generation step, or long incubation time for sample testing 27–39 .…”

A novel COOH–GO–COOH–MWNT/pDA/AuNPs based electrochemical aptasensor for detection of AFB1

“…[10][11][12] At present, many aptamer sensors capable of rapid detection in the eld have been developed, showing great potential in the safe detection of AFB 1 . [13][14][15][16][17][18][19][20][21][22][23][24] For example, Abnous et al developed a p-shaped structure composed of an aptamer and its complementary strand as a physical barrier on the electrode surface. 25 When AFB 1 is detected, the p-shaped structure is broken down by exonuclease I, and a change in peak current can be observed.…”