A short-term evaluation of semen and accessory sex gland function in phase III trial subjects receiving intravasal contraceptive RISUG

Chaki, Sankar P.; Das, Hem Chandra; Misro, M. M.

doi:10.1016/s0010-7824(02)00442-0

Cited by 34 publications

(18 citation statements)

References 7 publications

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“…Promising in vivo tests with SMA on rabbits were published recently [8]. Results of ongoing extended phase III clinical trials with SMA may substantiate its potential as a long-acting yet reversible male contraceptive procedure [27]. Despite all these constructive interventions, our findings are only at its early stage of development.…”

Section: Discussionmentioning

confidence: 86%

Is Endoscopic Vasectomy Just a Dream: An ex vivo Study on Feasibility and Reliability of Endoluminal Occlusion of Porcine Vas Deferens

Schlager

Maas²,

Hein³

et al. 2017

Urol Int

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Introduction and Aim: Surgical vasectomy remains the gold standard for fertility control in men. Endoluminal occlusion of the seminal ducts, thus avoiding an external incision, may become an appealing alternative to this approach. As our group has shown, nontraumatic endoscopic inspection of the seminal ducts is feasible in the human cadaver. We investigated the feasibility and reliability of occlusion using several commercially available medical sealing agents in the porcine vas deferens (VD). Methods and Main Outcome Measures: Tests were conducted using 25 porcine spermatic ducts (10 cm length) ex vivo. The explanted specimens were fixed and cannulated using the Seldinger technique. We administered 5 different agents (n-butyle-2-cyanoacrylate, n-butyle-2-cyanoacrylate in combination with a platinum vascular coil, Tissucol Duo S®, Gelita Spon® and AFP Plug®) endoluminally. Tightness was evaluated after 5, 15, 60, 360, 720, and 1,440 min for each of the five grades, respectively, using a solution of methylene blue and saline injected under controlled pressure of 300 mm Hg followed by histological examination. Results: All agents were administered into the porcine seminal ducts (4 out of 5 via a ureteric catheter). Gelita Spon® and Tissucol Duo S® did not occlude the lumen sufficiently, whereas n-butyle-2-cyanoacrylate, n-butyle-2-cyanoacrylate in combination with coil, Tissucol Duo and AFP Plug® performed satisfactorily. In particular, cyanoacrylate combined with a coil was able to close the seminal duct tightly and for a long time. Histological findings confirmed this sealant's gapless adhesion. AFP Plug® application revealed similarly good results. However, its form needs to be optimized to ensure its suitability for endoscopic use. Conclusion: Various developments regarding minimally invasive fertility control methods have been underway for decades. Further miniaturization of endoscopy and novel materials may pave the way for endoscopic fertility control in males in the future. We demonstrated the potential of commercially available medical sealing agents to reliably occlude the porcine VD.

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Section: Discussionmentioning

confidence: 86%

Is Endoscopic Vasectomy Just a Dream: An ex vivo Study on Feasibility and Reliability of Endoluminal Occlusion of Porcine Vas Deferens

Schlager

Maas²,

Hein³

et al. 2017

Urol Int

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“…32,33 RISUG is presently undergoing extended phase III clinical trials in India 2,34 after successfully completing phase I and phase II trials. 33,[35][36][37] The presence of common parent component SMA in Smart RISUG ensures long-term safety and safe reversibility of the drug.…”

Section: Discussionmentioning

confidence: 99%

Smart RISUG: A potential new contraceptive and its magnetic field-mediated sperm interaction

Jha¹,

Jha²,

Guha³

2009

IJN

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Abstract:The rationale and technique underlying a novel concept of noninvasive fertility control by a new Cuproferrogel contraceptive drug, iron oxide-copper-styrene maleic anhydride-dimethyl sulphoxide (Fe 3 O 4 -Cu-SMA-DMSO) composite named 'Smart RISUG' (smart reversible inhibition of sperm under guidance) in presence of pulsed magnetic fi eld (PMF; 1 mT to 800 mT) is explained. It was synthesized by dispersing iron oxide particles and copper particles into SMA-DMSO (male contraceptive RISUG) and characterized for particle distribution, particle size measurement and transmittance peaks, etc. Interaction of the RISUG particles as well as Smart RISUG particles with Albino rat sperm cell was studied in presence as well as absence of PMF. To fi nd an explanation to increased reaching of the Smart RISUG drug into sperm under infl uence of magnetic fi eld, the transport properties were characterized by high resolution transmission electron microscopy and atomic force microscopy. Smart RISUG could be mobilized into sperm cell membrane at the PMF, 760 mT in about 50 seconds. Adoption of novel drug Smart RISUG involving new technique may open the pathway for non surgical control of drug distribution, detection and restoration of the normal fertility after removal of the contraceptive from the male/female reproductive tube in presence of electromagnetic fi eld.

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“…Optimal epididymal function, with total alpha-glucosidase activity in seminal plasma > 83 IU=ml, is associated with a high probability of success of intra-uterine insemination (IUI) [3,6]. Seminal alpha-glucosidase level could reflect the effect of male contraception [1] and be used to evaluate the treatment effect of some drugs for obstructive occlusion [13]. To improve the liquefaction of semen samples, chymotrypsin or trypase was often added into semen samples [18,21].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Chymotrypsin on the Determination of Total Alpha-Glucosidase Activity in Seminal Plasma and the Correlation Between Alpha-Glucosidase Level and Semen Parameters

Xu¹,

Lü²,

Chen³

et al. 2006

Archives of Andrology

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& To evaluate the effect of chymotrypsin on the examination of alpha-glucosidase activity in seminal plasma, thirty-nine samples of fresh liquefied semen with or without chymotrypsin and forty-eight samples of fresh un-liquefied semen with chymotrypsin were determined for the total alpha-glucosidase activity in seminal plasma. The total alpha-glucosidase level of each sample was assayed by the method of glucose oxidase. The correlations between alpha-glucosidase level and semen parameters, including semen volume, pH, sperm concentration, grade a and b motility and total motility, were analyzed with SPSS 11.0 software. The results showed that chymotrypsin had no effect on seminal alpha-glucosidase activity determination. Chymotrypsin could improve the liquefaction for un-liquefied semen, and there was no significant difference of alpha-glucosidase activity between liquefied and un-liquefied semen samples. There were significantly positive correlations between seminal alpha-glucosidase activity (U=ml) and sperm concentration (r ¼ 0.338, p ¼ 0.015) and between total alpha-glucosidase activity (U=ejaculate) and semen volume (r ¼ 0.677, p ¼ 0.000). However, there was no significant correlation between alpha-glucosidase level (U=ml) and semen volume, pH, sperm motility or grade a and b motility (r ¼ À0.234 $ 0.077, p ¼ 0.099 $ 0.993). The data indicated that chymotrypsin could be added into the Address correspondence to Dr. Jin-Chun Lu, Department of Reproduction and Genetics, Nanjing General Hospital, PLA, 305 East Zhongshan Road, Nanjing 210002, P. R. China. E-mail: jclu@jlonline. un-liquefied semen samples for alpha-glucosidase activity determination, and there were different correlations between seminal alpha-glucosidase level and various semen parameters.

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A short-term evaluation of semen and accessory sex gland function in phase III trial subjects receiving intravasal contraceptive RISUG

Cited by 34 publications

References 7 publications

Is Endoscopic Vasectomy Just a Dream: An ex vivo Study on Feasibility and Reliability of Endoluminal Occlusion of Porcine Vas Deferens

Is Endoscopic Vasectomy Just a Dream: An ex vivo Study on Feasibility and Reliability of Endoluminal Occlusion of Porcine Vas Deferens

Smart RISUG: A potential new contraceptive and its magnetic field-mediated sperm interaction

The Effect of Chymotrypsin on the Determination of Total Alpha-Glucosidase Activity in Seminal Plasma and the Correlation Between Alpha-Glucosidase Level and Semen Parameters

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