“…These results suggest that 1-3 cause the death of HL-60 cells through an apoptotic process. Compounds 1-3 also showed antiproliferative activity in a dose-dependent manner [1: 10 lg/ml (viability 84%), 30 (14), 100 (1); 2: 10 (83), 30 (50), 100 (2); 3: 10 (91), 30 (10), 100 (1.5)] and IC 50 values were 22, 34, and 21 lg/ml in HCT-15 cells. The terminal diol moiety of the chain structure might therefore be important for the highly selective antiproliferative property of 1-3, because fractions including linear polyacetylene compounds, callyberynes A-C, showed very weak antiproliferative activity (%growth suppression of this fraction: 0% at 30 lg/ml, 30% at 100 lg/ml), but fractions including 1-3 showed stronger antiproliferative activity than those of callyberynes A-C (%growth suppression of this fraction: 60% at 30 lg/ml, 100% at 100 lg/ml; Fig.…”