A Short Period of Mannitol Stress but Not LiCl Stress Led to Global Translational Repression in Plants

Matsuura, Hideyuki; Ueda, Ken–ichi; Ishibashi, Yoïchi; Kubo, Yuki; Yamaguchi, Masatoshi; Hirata, Kazumasa; Demura, Taku; Kato, Ko

doi:10.1271/bbb.100330

Cited by 10 publications

(11 citation statements)

References 8 publications

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“…It falls to less than 25% during the night in the starchless pgm mutant. Values of 20% to 25% resemble those seen under a range of stress treatments, including dehydration (Hsiao, 1970;Scott et al, 1979;Kawaguchi et al, 2003Kawaguchi et al, , 2004Kawaguchi and Bailey-Serres, 2005;Matsuura et al, 2010), anaerobiosis (Branco- Price et al, 2005Price et al, , 2008Mustroph et al, 2009), and severe C depletion (Nicolaï et al, 2006). We conclude that there are substantial changes in overall polysome loading during an undisturbed diurnal cycle and that exhaustion of starch leads to a decrease comparable to that seen under extreme stress.…”

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confidence: 54%

Diurnal Changes of Polysome Loading Track Sucrose Content in the Rosette of Wild-Type Arabidopsis and the Starchless pgm Mutant

et al. 2013

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Growth is driven by newly fixed carbon in the light, but at night it depends on reserves, like starch, that are laid down in the light. Unless plants coordinate their growth with diurnal changes in the carbon supply, they will experience acute carbon starvation during the night. Protein synthesis represents a major component of cellular growth. Polysome loading was investigated during the diurnal cycle, an extended night, and low CO 2 in Arabidopsis (Arabidopsis thaliana) Columbia (Col-0) and in the starchless phosphoglucomutase (pgm) mutant. In Col-0, polysome loading was 60% to 70% in the light, 40% to 45% for much of the night, and less than 20% in an extended night, while in pgm, it fell to less than 25% early in the night. Quantification of ribosomal RNA species using quantitative reverse transcription-polymerase chain reaction revealed that polysome loading remained high for much of the night in the cytosol, was strongly light dependent in the plastid, and was always high in mitochondria. The rosette sucrose content correlated with overall and with cytosolic polysome loading. Ribosome abundance did not show significant diurnal changes. However, compared with Col-0, pgm had decreased and increased abundance of plastidic and mitochondrial ribosomes, respectively. Incorporation of label from 13 CO 2 into protein confirmed that protein synthesis continues at a diminished rate in the dark. Modeling revealed that a decrease in polysome loading at night is required to balance protein synthesis with the availability of carbon from starch breakdown. Costs are also reduced by using amino acids that accumulated in the previous light period. These results uncover a tight coordination of protein synthesis with the momentary supply of carbon.

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confidence: 54%

Diurnal Changes of Polysome Loading Track Sucrose Content in the Rosette of Wild-Type Arabidopsis and the Starchless pgm Mutant

et al. 2013

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“…In many eukaryotic organisms, bulk translation is impeded when an unfavorable physical environment compromises translation or processing. Likewise, in plants, many abiotic stresses, such as osmotic stress (Kawaguchi et al, 2003;Matsuura et al, 2010b), cause a dramatic change in polysome loading that affects the bulk of cellular mRNAs. The effects on translation of several environmental conditions have been examined at the global level in Arabidopsis; heat and salt (Matsuura et al, 2010a), drought (Kawaguchi et al, 2004), cadmium heavy metal (Sormani et al, 2011b), hypoxia (Branco-Price et al, 2005;Branco-Price et al, 2008;Mustroph et al, 2009), light and darkness (Juntawong and Bailey-Serres, 2012;Liu et al, 2012), as well as sucrose deprivation (Nicolai et al, 2006).…”

Section: Translational Control In Response To Abiotic Stressmentioning

confidence: 99%

Translational Regulation of Cytoplasmic mRNAs

Roy

Arnim

2013

The Arabidopsis Book

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Translation of the coding potential of a messenger RNA into a protein molecule is a fundamental process in all living cells and consumes a large fraction of metabolites and energy resources in growing cells. Moreover, translation has emerged as an important control point in the regulation of gene expression. At the level of gene regulation, translational control is utilized to support the specific life histories of plants, in particular their responses to the abiotic environment and to metabolites. This review summarizes the diversity of translational control mechanisms in the plant cytoplasm, focusing on specific cases where mechanisms of translational control have evolved to complement or eclipse other levels of gene regulation. We begin by introducing essential features of the translation apparatus. We summarize early evidence for translational control from the pre-Arabidopsis era. Next, we review evidence for translation control in response to stress, to metabolites, and in development. The following section emphasizes RNA sequence elements and biochemical processes that regulate translation. We close with a chapter on the role of signaling pathways that impinge on translation.

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“…The rest of the transcriptionally regulated genes cover multiple functions, such as transcription, translation, signaling, metabolism and general stress response. However, this mRNA steady-state scenario may not reflect the protein level output, since, after being transcribed, the mRNAs should be translated and translation is also widely altered in these conditions [25], [26], [27], [28]. In this regard, recent analyses done in Arabidopsis and O. sativa cultured cells have pointed out that, upon short heat stress treatments, translation is generally and specifically regulated [27], [28].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Genome-Wide Changes in the Translatome of Arabidopsis Seedlings Subjected to Heat Stress

et al. 2013

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Heat stress is one of the most prominent and deleterious environmental threats affecting plant growth and development. Upon high temperatures, plants launch specialized gene expression programs that promote stress protection and survival. These programs involve global and specific changes at the transcriptional and translational levels. However, the coordination of these processes and their specific role in the establishment of the heat stress response is not fully elucidated. We have carried out a genome-wide analysis to monitor the changes in the translation efficiency of individual mRNAs of Arabidopsis thaliana seedlings after the exposure to a heat shock stress. Our results demonstrate that translation exerts a wide but dual regulation of gene expression. For the majority of mRNAs, translation is severely repressed, causing a decreased of 50% in the association of the bulk of mRNAs to polysomes. However, some relevant mRNAs involved in different aspects of homeostasis maintenance follow a differential pattern of translation. Sequence analyses of the differentially translated mRNAs unravels that some features, such as the 5′UTR G+C content and the cDNA length, may take part in the discrimination mechanisms for mRNA polysome loading. Among the differentially translated genes, master regulators of the stress response stand out, highlighting the main role of translation in the early establishment of the physiological response of plants to elevated temperatures.

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A Short Period of Mannitol Stress but Not LiCl Stress Led to Global Translational Repression in Plants

Cited by 10 publications

References 8 publications

Diurnal Changes of Polysome Loading Track Sucrose Content in the Rosette of Wild-Type Arabidopsis and the Starchless pgm Mutant

Diurnal Changes of Polysome Loading Track Sucrose Content in the Rosette of Wild-Type Arabidopsis and the Starchless pgm Mutant

Translational Regulation of Cytoplasmic mRNAs

Analysis of Genome-Wide Changes in the Translatome of Arabidopsis Seedlings Subjected to Heat Stress

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