A short hypoosmotic swelling test for the prediction of boar sperm fertility

Llano, Begoña Pérez; Lorenzo, J.L.; Yenes, P.; Trejo, A.; Garcı́a-Casado, Pedro

doi:10.1016/s0093-691x(01)00571-4

Cited by 87 publications

(61 citation statements)

References 23 publications

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“…The percentage of spermatozoa responding by swelling was lower than the percentage of motility and living spermatozoa. These relations were in agreement with Vazquez et al (1997) and Pérez-Llano et al (2001). The difference may be due to the fact that some spermatozoa with membrane damage remain motile (Zou et al, 2000) or the membranes of some spermatozoa are inactivated when they come into contact with the hypoosmotic solution.…”

Section: Discussionsupporting

confidence: 68%

“…HOST enables to evaluate the functional status of the sperm membrane. Pérez-Llano et al (2001) reported a significant correlation of sHOST with in vivo fertility and with farrowing rate. The activity of aspartate aminotransferase (AST) is another interesting marker used to indicate sperm damage.…”

mentioning

confidence: 97%

“…In the present study besides traditional methods (sperm motility and percentage of viable spermatozoa) short hypoosmotic swelling test (sHOST) and the activity of the enzyme aspartate aminotransferase (AST) were applied for an assessment of the sperm membrane integrity. The short hypoosmotic swelling test (sHOST) is a modification of HOST (Pérez-Llano et al, 2001). This test is based on the semi-permeability of the intact cell membrane that allows the sperm to swell under hypoosmotic conditions.…”

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confidence: 99%

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Effects of long-term liquid commercial semen extender and storage time on the membrane quality of boar semen

Frydrychová¹,

Čeřovský²,

Lustyková³

et al. 2010

CZECH J ANIM SCI

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ABSTRACT:The objective of this study was to assess the sperm membrane integrity in extended boar semen during storage time using specific spectrum laboratory methods. Boar semen was diluted with the long-term liquid commercial extenders Androhep (A), Androstar (AS), Androstar plus (AS + ), LD and M III and was stored up to 96 h. The sperm membrane integrity was evaluated by motility, viable spermatozoa, short hypoosmotic swelling test (sHOST) and by the activity of the enzyme aspartate aminotransferase (AST). Negative changes in the quality of sperm membrane in relation to storage time were observed after 48 h for sHOST, after 72 h for viable spermatozoa and after 72 h for motility. The percentage of viable spermatozoa was decreased by 0.27% each hour. A statistically significant difference between extenders A and LD was observed in sHOST after 72 h and 96 h storage (P < 0.05). The AST activity did not show any statistically significant differences in extenders and in storage time. In overall assessment Androhep was the best of the tested extenders, followed by AS, AS + , M III and LD in terms of motility, viable spermatozoa and sHOST. The correlations among laboratory methods were highly significant (P < 0.001). In conclusion, the results documented that the sperm membrane integrity was statistically significantly affected by extenders and storage time (P < 0.001). Boar semen quality was the best in extender A. sHOST is a very sensitive and relatively simple method for the assessment of sperm membrane integrity in diluted semen.

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Section: Discussionsupporting

confidence: 68%

mentioning

confidence: 97%

mentioning

confidence: 99%

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Effects of long-term liquid commercial semen extender and storage time on the membrane quality of boar semen

Frydrychová¹,

Čeřovský²,

Lustyková³

et al. 2010

CZECH J ANIM SCI

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“…Tartaglione and Ritta (2004) included an eosin-nigrosin supravital stain combined with the HOS test into a regression equation as a predictor of in vitro fertility of FT bull semen. Pérez-Llano et al (2001) also included the HOS test in a regression equation along with other semen parameters such as morphology, motility, and acrosome integrity to predict the fertility of boar semen. Contrarily, also working with boars, Gadea and Matas (2000) found that the results of the HOS test were not significant when regression models for fertilization rate were evaluated.…”

Section: Discussionmentioning

confidence: 99%

Relationships between the results of hypo-osmotic swelling tests, sperm motility, and fertility in Estonian Holstein dairy bulls

Padrik¹,

Hallap²,

Kaart³

et al. 2012

Czech J. Anim. Sci.

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ABSTRACT:As an attempt to find an inexpensive and simple laboratory method for artificial insemination (AI) bull semen quality assessment, the osmotic resistance of spermatozoa was measured using the hypoosmotic swelling (HOS) test, developed by Jeyendran et al. (1984) (labelled HOS-1), and its modifications (HOS-2, HOS-3), with decreased osmotic pressure aimed at challenging sperm survival ability. The test results were benchmarked against sperm viability measurements performed using the Computerized Motility Analyzer (CMA), and field fertility was calculated as non-return rate (NRR). Two age groups of Estonian Holstein bull sires were included in this study to test possible age effect on semen quality parameters. The HOS-1 test in fresh bull semen correlated well with sperm general motility (GMot) (r = 0.63, P < 0.001 at batch level and r = 0.77, P < 0.001 at bull level) as well as with progressive motility (PMot) in frozen-thawed (FT) semen (r = 66, P < 0.001 at batch level and r = 0.81, P < 0.001 at bull level), which makes the test suitable for the prediction of post-thaw semen quality. However, the HOS-2 and HOS-3 values in FT semen had high correlations with NRR (r = 0.65, r = 0.66, P < 0.001 at batch level and r = 0.63, r = 0.71, P < 0.01 at bull level), which was comparable to those between GMot and NRR or PMot and NRR. A combination of motility parameters and the results of the HOS-1 and HOS-3 tests provided a good model for predicting the potential fertility of bull semen. Values of sperm membrane post-thaw intactness, assessed using HOS-2, as well as of sperm motility measurements were higher in mature bulls compared to those in young bulls. Short conclusion: different modifications of the hypo-osmotic swelling test are useful for routine bovine semen quality assessment at AI stations.Keywords: bull fertility; semen quality; sperm membrane intactness; bull age Laboratory analyses have been used over many decades to evaluate semen quality. Despite the fact that none of the single assays developed provide results that consistently and highly correlate with fertility, the laboratory evaluation of semen samples remains an important procedure for the artificial insemination (AI) industry to eliminate low fertility bulls or semen from being used in artificial insemination programmes (Graham and Mocé, 2005).Post-thaw viability and fertility of cryopreserved sperm are often reduced due to accumulated cellular damage during the cryopreservation phases (Muiño et al., 2008). Decrease in temperature, cold shock, and intracellular ice formation can affect sperm plasma membrane, acrosomal and mitochondrial membrane integrity (Thomas et al., 1998;Defoin et al., 2008), and cause the loss of intracellular components (Graham and Mocé, 2005), which can initiate cell death.Sperm membrane integrity can be evaluated by several methods, e.g. light or fluorescent microscopy combined with vital stains (Brito et al., 2003), and flow cytometry (Januskauskas et al.,

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“…3) (Neild et al, 1999). The HOS test is a simple, inexpensive and easily applicable technique, which has been adapted to assess spermatozoa of several species (Corea & Zavos, 1994;Kumi-Diaka, 1993Neild et al, 1999Pérez-Llano et al, 2001;Santiago-Moreno et al, 2009). It has been suggested that this test may supplement the information provided by the conventional parameters of semen analysis, and is useful for fertilizing ability assessment (Brito et al, 2003;Vazquez et al, 1997).…”

Section: Sperm Membrane Integritymentioning

confidence: 99%