A Short Human and Mouse MLR Assay Utilizing Lymphokine (Il-2, Il-3) Secretion as an Early Activation Event

Bishara, Amal; Kedar, Eli; Leshem, B.; E, Rosenkovitch; Brautbar, Chaim

doi:10.1097/00007890-199105000-00033

Cited by 12 publications

(7 citation statements)

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“…Earlier diagnosis (within 72 h) may also be possible, by analysis of aniigenspecifie proliferation or cylokine production. The latter has already found clinical application in transplantation immunology, with the rapid interpretation of results of the mixed lymphocyte reaction [24].…”

Section: Discussionmentioning

confidence: 99%

Cellular immunity in tuberculous pleural effusions: evidence of spontaneous lymphocyte proliferation and antigen-specific accelerated responses to purified protein derivative (PPD)

Lorgat

Keraan

Ress

1992

Clinical and Experimental Immunology

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SUMMARYThe kinetics of in vitro cellular proliferation against a PPD of Mycohacterium tuberculosis or slreptococcal antigen (strepiokinase-streptodornase) was evaluated in pleural fiuid and peripheral blood mononuclear cells (PBMC) from patients with tuberculous and non-tuberculous pleuritis. The peak proliferati ve response lo PPD by mononuclear cells from pleural fluid occurred earlier (day 3) in 65% of patients with tuberculosis, a finding not seen in non-tuberculous effusions. Spontaneous lyniphocylc proliferation of both peripheral blood lymphocytes and pleural effusion lymphocytes was frequently observed, irrespective of etiology. However, 20 of 21 tuberculous patients manifesting spontaneous lymphocyte proliferation had accelerated kinetics of proliferation to PPD. which was antigen-specific. These results suggest that spontaneous lymphocyte proliferation occurs as a response to antigen stimulation at the site of disease, and is not a non-specific response to inflammation. Furthermore, enhanced reactivity against mycobacterial antigen, manifested by accelerated kinetics of proliferation, has diagnostic potential in patients with pleural effusions.

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Section: Discussionmentioning

confidence: 99%

Cellular immunity in tuberculous pleural effusions: evidence of spontaneous lymphocyte proliferation and antigen-specific accelerated responses to purified protein derivative (PPD)

Lorgat

Keraan

Ress

1992

Clinical and Experimental Immunology

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“…The colorimetric MTT technique [15] was employed for this purpose. Single splenocyte suspensions were used.…”

Section: One Way Mlrmentioning

confidence: 99%

The effect of pregnancy on paternal skin allograft survival

Shou

Xu²,

Xiao³

et al. 2009

SCI CHINA SER C

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Elucidation of maternal-fetal tolerance mechanisms clarifies the role of regulatory T cells (Treg) in transplant tolerance. This study aim to investigate the effect of pregnancy on paternal skin allograft survival. Flow cytometry techniques, mixed lymphocytes reaction (MLR), PCR, real-time PCR and skin transplantation were key methods. Treg increased significantly from 4.2% before pregnancy to peak at 6.8% day 8 after pregnancy. Both heme oxygenase-1 (HO-1) and indoleamine 2,3-dioxygenase (IDO) mRNA express high in placenta while low in spleen (P<0.05). Although Treg increased during pregnancy, and splenocytes from the pregnant mice showed lower MLR response toward the paternal stimulator, single time pregnancy showed no significant protective effect on paternal skin allograft survival in the tested condition.

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“…Liposomes were separated from the nonencapsulated protein by centrifugation, and encapsulation efficiency was determined by bioassay for IL-2 [Bishara et al, 1991;Kedar et al, 1994] and by protein assay for HN by Peterson's modification [Peterson, 1983] of the method of Lowry et al [1951]. Encapsulation was !80% for IL-2 and 60-80%, depending on the viral strain, for HN.…”

Section: Liposomal Vaccine (Influsome-vac)mentioning

confidence: 99%

“…Neutralizing anti-IL-2 antibodies were assessed by bioassay, using the IL-2-dependent murine T-cell line CTLL-2 [Bishara et al, 1991;Kedar et al, 1994]. Briefly, recombinant human IL-2 (50 ml, 2 ng/ml, Chiron, Emeryville, CA) was mixed with 50 ml of heatinactivated sera from vaccinees, diluted 1:2-1:256 in 96-well flat-bottomed microtiter plates, and incubated at 378C for 1 hr.…”

Section: Assaysmentioning

confidence: 99%

Immunogenicity and safety of a novel liposomal influenza subunit vaccine (INFLUSOME‐VAC) in young adults

Ben‐Yehuda

Joseph

Zeira

et al. 2003

Journal of Medical Virology

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Influenza and its complications account for substantial morbidity and mortality among young adults and especially among the elderly. In young adults, immunization provides 70-90% protection, while among the elderly the vaccine may be only 30-40% effective; hence the need for new, more immunogenic vaccines. We compared the safety and immunogenicity of a novel IL-2-supplemented liposomal influenza vaccine (designated INFLUSOME-VAC) with that of a commercial subunit vaccine and a commercial split virion vaccine in young adults (mean age 28 years) in the winter of 1999-2000. Seventy-three healthy young adults were randomly assigned to be vaccinated intramuscularly with the following: a commercial subunit vaccine (n = 17, group A), INFLUSOME-VAC (n = 36, group B), and a commercial split virion vaccine (n = 20, group C). The three vaccines contained equal amounts of hemagglutinin (approximately 15 microg each) from the strains A/Sydney (H3N2), A/Beijing (H1N1), and B/Yamanashi. INFLUSOME-VAC induced higher geometric mean HI titers and higher-fold increases in HI titers against all three strains, compared with the two commercial vaccines. In addition, seroconversion rates for the A/Sydney and B/Yamanashi strains were significantly higher (P< 0.05) compared with the split virion vaccine, and significantly higher for the three strains compared with the subunit vaccine (69-97% vs 35-65%, P < or = 0.02). Moreover, the anti-neuraminidase response was significantly greater (P = 0.05) in group B vs group A. INFLUSOME-VAC caused mild local pain at the injection site in a significantly higher proportion of the vaccinees (83%). Thus, INFLUSOME-VAC is an immunogenic and safe vaccine in young adults.

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A Short Human and Mouse MLR Assay Utilizing Lymphokine (Il-2, Il-3) Secretion as an Early Activation Event

Cited by 12 publications

References 0 publications

Cellular immunity in tuberculous pleural effusions: evidence of spontaneous lymphocyte proliferation and antigen-specific accelerated responses to purified protein derivative (PPD)

Cellular immunity in tuberculous pleural effusions: evidence of spontaneous lymphocyte proliferation and antigen-specific accelerated responses to purified protein derivative (PPD)

The effect of pregnancy on paternal skin allograft survival

Immunogenicity and safety of a novel liposomal influenza subunit vaccine (INFLUSOME‐VAC) in young adults

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