A short history of the CaMV 35S promoter

Somssich, Marc

doi:10.7287/peerj.preprints.27096v1

Cited by 4 publications

(3 citation statements)

References 72 publications

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“…To overcome these constraints, we attempted syringe infiltration with recombinant Agrobacterium, containing vectors for in planta GFP expression, at different stages of development in sorghum leaves. As expected, strength of signal in sorghum leaves was higher with the maize ubiquitin promoter as compared to cauliflower mosaic virus 35S promoter, which is reported to perform better in dicots [38]. In our system, although infiltration medium could enter the mature leaves, GFP expression was only detected in the infiltrated younger leaves of 3-4-week-old plants.…”

Section: Discussionsupporting

confidence: 71%

Agrobacterium-mediated transient transformation of sorghum leaves for accelerating functional genomics and genome editing studies.

Sharma

Liang

Lee

et al. 2019

Preprint

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Objectives Sorghum is one of the most recalcitrant species for transformation. Considering the time and effort required for stable transformation in sorghum, establishing a transient system to screen the efficiency and full functionality of vector constructs is highly desirable. Results Here, we report an Agrobacterium-mediated transient transformation assay with intact sorghum leaves using green fluorescent protein as marker. It also provides a good monocot alternative to tobacco and protoplast assays with a direct, native and more reliable system for testing single guide RNA (sgRNA) expression construct efficiency. Given the simplicity and ease of transformation, high reproducibility, and ability to test large constructs, this method can be widely adopted to speed up functional genomic and genome editing studies.

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Section: Discussionsupporting

confidence: 71%

Agrobacterium-mediated transient transformation of sorghum leaves for accelerating functional genomics and genome editing studies.

Sharma

Liang

Lee

et al. 2019

Preprint

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“…The proposed selfattenuation mechanism provides CaMV with the means to balance host fitness and virus replication during prolonged infections, a battle that will entail many host factors which remain to be identified. The CaMV 35S promoter is invaluable for molecular biology and "since its description in 1985 [...] has been the standard promoter used in all plant science and plant biotechnology, and has certainly propelled the research field forward like hardly any other discovery" (direct quote from Somssich, 2019). While the economic impact of CaMV infections pales in comparison to other plant viruses, it occurs widespread in agriculturally used fields, causes symptoms, and can lead to drastic yield losses when co-infecting with other viruses (Bak & Emerson, 2020;Farzadfar et al, 2014;Spence et al, 2007).…”

Section: Self-attenuation -A Viral Balancing Actmentioning

confidence: 99%

Stress & Granules : regulation of Cauliflower mosaic virus disease in Arabidopsis thaliana

Hoffmann¹

2023

Acta Universitatis Agriculturae Sueciae

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Viruses exist in intimate relationships with the organisms they are infecting and are just as dependent on compatibility with their host’s cellular components as they are on subverting them. During any virus infection, the cells are flooded with foreign nucleic acids (DNA and/or RNA) and have learned to recognize, disarm, or eliminate them. In turn, viruses have evolved to use the cellular transcription and RNA regulatory machinery for their own benefit. Cytoplasmic RNA granules, namely Processing bodies (PBs) and Stress granules (SGs) are at the forefront of RNA regulation as they contain and store untranslated RNA and are responsive in number, size, and composition to various stresses, including virus infection. For this thesis, we have explored the role of Arabidopsis thaliana RNA granules during infection with the pararetrovirus Cauliflower mosaic virus (CaMV). We show that PB components aid virus accumulation through shielding of the viral RNA from the antiviral RNA silencing machinery (Paper I). In addition, we find that the cytoplasmic viral replication factory contains several RNA granule proteins during infection, including both, PB components and SG components. Opposite to PBs, SGs are likely antiviral and CaMV subverts their biogenesis through its multifunctional protein P6 (Paper II). In an effort to uncover novel disease determinants, we explore the variation of CaMV disease in naturally occurring populations of Arabidopsis thaliana and uncover the importance of the plant hormone abscisic acid and its homeostasis for CaMV infection, as well as a novel CaMV susceptibility factor, the ABA synthesis gene NCED9 (Paper III).

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“…These, however, were not active in all plant tissues and are under strong developmental and environmental regulation, resulting in high variability in their activity and limiting their usefulness in transgenesis (An, Costa, Mitra, Ha, and Márton, 1988). This situation changed in 1985 with the description of the cauliflower mosaic virus (CaMV) 35S promoter (Odell, Nagy, and Chua, 1985; Somssich, 2018 b ).…”

Section: Toward Plant Molecular Biology and Biotechnologymentioning

confidence: 99%

The Dawn of Plant Molecular Biology: How Three Key Methodologies Paved the Way

Somssich

2022

Current Protocols

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The adoption of Arabidopsis thaliana in the 1980s as a universal plant model finally enabled researchers to adopt and take full advantage of the molecular biology tools and methods developed in the bacterial and animal fields since the early 1970s. It further brought the plant sciences up to speed with other research fields, which had been employing widely accepted model organisms for decades. In parallel with this major development, the concurrent establishment of the plant transformation methodology and the description of the cauliflower mosaic virus (CaMV) 35S promoter enabled scientists to create robust transgenic plant lines for the first time, thereby providing a valuable tool for studying gene function. The ability to create transgenic plants launched the plant biotechnology sector, with Monsanto and Plant Genetic Systems developing the first herbicide‐ and pest‐tolerant plants, initiating a revolution in the agricultural industry. Here I review the major developments over a less than 10‐year span and demonstrate how they complemented each other to trigger a revolution in plant molecular biology and launch an era of unprecedented progress for the whole plant field. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC.

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A short history of the CaMV 35S promoter

Cited by 4 publications

References 72 publications

Agrobacterium-mediated transient transformation of sorghum leaves for accelerating functional genomics and genome editing studies.

Agrobacterium-mediated transient transformation of sorghum leaves for accelerating functional genomics and genome editing studies.

Stress & Granules : regulation of Cauliflower mosaic virus disease in Arabidopsis thaliana

The Dawn of Plant Molecular Biology: How Three Key Methodologies Paved the Way

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