A short guide to insect oviposition: when, where and how to lay an egg

Cury, Kevin M.; Prud’homme, Benjamin; Gompel, Nicolas

doi:10.1080/01677063.2019.1586898

Cited by 64 publications

(59 citation statements)

References 141 publications

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“…We next screened a library of LexA lines 34 to identify candidate sensory neurons that detect the progression of the egg 12,[35][36][37] . We identified three lines that drive expression in a cluster of peripheral neurons whose processes intercalate with muscle fibers that encircle the distal-most aspect of the uterus (Posterior Uterine sensory, or PU neurons) (Figure S2A).…”

Section: Pu Sensory Neurons Are Activated By the Presence Of The Egg In The Ovipositormentioning

confidence: 99%

“…Considerable pressure is imposed on the selection of the appropriate time and place to deposit eggs. Fruit flies express robust and species-specific preferences for the site of egg deposition based in part on odor, taste, and texture [8][9][10][11][12][13][14][15] . During egg-laying, females evaluate the local environment prior to expressing an ordered motor sequence that culminates in egg deposition subterraneously within a nutritive substrate 14,[16][17][18] .…”

Section: Introductionmentioning

confidence: 99%

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Decisions in an Innate Behavioral Sequence

2021

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Innate behaviors are comprised of ordered sequences of component actions that progress to satisfy drives. We have characterized the structure of egg-laying behavior in Drosophila in detail and observed that the sequence is not merely comprised of motor acts but also acts of sensory exploration that govern the transitions between component actions. We have identified a cluster of internal sensory neurons that provide information about the progression of the egg during ovipositor burrowing, a behavior necessary for the subterraneous deposition of the egg. These neurons impart sensory feedback that allows burrowing to continue to egg deposition or to abort in favor of further exploration. Diminished activity of these neurons upon completed egg expulsion may initiate the transition to the final phase of egg-laying, allowing the cycle to repeat. Sensory feedback therefore plays a critical role at decision points between transitions affording innate behaviors with an adaptive flexibility.

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Section: Pu Sensory Neurons Are Activated By the Presence Of The Egg In The Ovipositormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Decisions in an Innate Behavioral Sequence

2021

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“…They thrive in humid habitats, such as under decaying leaf matter, dung piles or fallen dead wood (Binns 1981 ; Jakovlev 2011 ; Mead and Fasulo 2001 ) and prefer to oviposit in microbe-rich media (Braun et al 2012 ). As generally with insects, volatiles are pivotal cues in finding the most favourable habitat for the next generation (Cury et al 2019 ). To identify a sufficient oviposition medium a vast array of environmental factors should be considered.…”

Section: Discussionmentioning

confidence: 99%

Fungal Volatiles as Olfactory Cues for Female Fungus Gnat, Lycoriella ingenua in the Avoidance of Mycelia Colonized Compost

et al. 2020

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The chemical signatures emitted by fungal substrates are key components for mycophagous insects in the search for food source or for suitable oviposition sites. These volatiles are usually emitted by the fruiting bodies and mycelia. The volatiles attract fungivorous insects, like flowers attract pollinators; certain flowers mimic the shape of mushroom fruiting bodies and even produce a typical mushroom odor to exploit on fungus-insect mutualism. There are numerous insects which are mycophagous or eat fungi additionally, but only a few are considered a threat in agriculture. Lycoriella ingenua is one of the most serious pests in mushroom cultivation worldwide. Here we attempt to examine the role of environmental volatiles upon behavioral oviposition preference. In two-choice bioassays, fungus gnats preferred uncolonized compost compared to colonized compost but preferred colonized compost against nothing. However, when colonized compost was paired against distilled water, no significant choice was observed. The comparison of fresh casing material and mycelium colonized casing material resulted in no significant preference. From colonized compost headspace, three antennally active volatiles were isolated by gas chromatography coupled with electroantennography and subsequently identified with gas chromatography coupled mass spectrometry as 1-hepten-3-ol, 3-octanone and 1-octen-3-ol. In behavioral assays the addition of said synthetic volatiles to uncolonized compost separately and in combination to mimic colonized compost resulted in avoidance. We thus partially elucidate the role of fungal volatiles in the habitat seeking behavior of Lycoriella ingenua.

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“…The activation of olfactory sensory neurons that express specific ORs can evoke egg-laying based on sensory cues, a process that might be inhibited by Orco knockdown. However, even mating-derived cues can promote such behaviors by engaging either chemosensation or mechanosensation [36]. In the first case, sperm transfer is the primary molecular trigger to be delivered by the male to the female.…”

Section: Knockdown Of Bo-orco Impairs Mate Recognition and Ovipositionmentioning

confidence: 99%

Anosmic flies: what Orco silencing does to olive fruit flies

et al. 2020

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Background The olive fruit fly (Bactrocera oleae) is the most destructive pest of the olive cultivation worldwide causing significant production losses and olive fruit impoverishment, as its larvae feed exclusively on the olive fruit. Reproductive and sexual behavior, as well as host-plant recognition of the fly, are highly dependent on its chemosensory system. Therefore, exploring the role of genes that play a critical role in olfaction, could reveal potential molecular targets that determine species-specific features on chemical communication and could be used to impair sexual behavior. Results In this study we identified the gene that encodes the conserved olfactory co-receptor Orco (Odorant receptor co-receptor), which interacts with all divergent insect odorant receptors, and investigated how disruption of its expression affects chemoreception. We initially searched the expression profile of Bo-Orco in both sexes during sexual maturation, as well as pre- and post-mating communication by relative quantitative real time PCR (qRT-PCR) analysis suggesting that Bo-Orco was abundantly expressed in sexually mature adults. We further investigated the functional role of Bo-Orco in mating and oviposition behavior via transient gene silencing that was performed through in vivo dsRNA hemolymph injections in sexually mature flies 7 days after eclosion. Orco-knockdown phenotypes in both sexes showed reduced copulation rates in mating competitiveness tests, possibly through impaired olfactory-mediated detection of sex pheromone. In addition, oviposition was significantly inhibited in dsRNA-Orco injected females in a post-mating behavior test. Conclusions Our results demonstrate that Orco plays a crucial role in the reproductive behavior of the olive fruit fly, since pre- and post-mating processes were affected. This is the first report in the olive fruit fly that links the chemosensory pathway with the mating behavior and the reproductive potential at a molecular basis, rendering this gene a potential target for the improvement of the olive fruit fly population control techniques.

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A short guide to insect oviposition: when, where and how to lay an egg

Cited by 64 publications

References 141 publications

Decisions in an Innate Behavioral Sequence

Decisions in an Innate Behavioral Sequence

Fungal Volatiles as Olfactory Cues for Female Fungus Gnat, Lycoriella ingenua in the Avoidance of Mycelia Colonized Compost

Anosmic flies: what Orco silencing does to olive fruit flies

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