A Shigella boydii bacteriophage which resembles Salmonella phage ViI

Abstract

BackgroundLytic bacteriophages have been applied successfully to control the growth of various foodborne pathogens. Sequencing of their genomes is considered as an important preliminary step to ensure their safety prior to food applications.ResultsThe lytic bacteriophage, ΦSboM-AG3, targets the important foodborne pathogen, Shigella. It is morphologically similar to phage ViI of Salmonella enterica serovar Typhi and a series of phages of Acinetobacter calcoaceticus and Rhizobium meliloti. The complete genome of ΦSboM-AG3 was determined to be 158 kb and was terminally redundant and circularly permuted. Two hundred and sixteen open reading frames (ORFs) were identified and annotated, most of which displayed homology to proteins of Salmonella phage ViI. The genome also included four genes specifying tRNAs.ConclusionsThis is the first time that a Vi-specific phage for Shigella has been described. There is no evidence for the presence of virulence and lysogeny-associated genes. In conclusion, the genome analysis of ΦSboM-AG3 indicates that this phage can be safely used for biocontrol purposes.

Characterization and Comparative Genomic Analysis of a Novel Bacteriophage, SFP10, Simultaneously Inhibiting both Salmonella enterica and Escherichia coli O157:H7

“…However, three host specificityrelated genes (orf00210, orf00213, and orf00214) of Shigella phage phiSboM-AG3 showed low homology with a phage Vi01 gene encoding receptor binding protein targeting Vi capsular antigen (Table 3). Recent genome sequence analysis of Shigella boydii revealed no gene for biosynthesis of Vi capsular antigen, suggesting that the receptor of phiSboM-AG3 may not be Vi capsular antigen (2). In addition, no Shigella-specific phage gene related to host specificity was detected in BLASTP analysis, probably due to lack of information on other known Shigella phage receptor binding proteins in the GenBank database.…”

Bacteriophage ϕMAM1, a Viunalikevirus, Is a Broad-Host-Range, High-Efficiency Generalized Transducer That Infects Environmental and Clinical Isolates of the Enterobacterial Genera Serratia and Kluyvera

“…Many reports have reported promiscuity in enterobacterial phages (34,35,60,67,68), and MAM1 is another noteworthy example. MAM1 was found to infect 68% of the Serratia marcescens strains tested and a rhizobacterial strain from the Kluyvera genus.…”

Biocontrol of Shigella flexneri in Ground Beef and Vibrio cholerae in Seafood with Bacteriophage-Assisted High Hydrostatic Pressure (HHP) Treatment

“…A bacteriophage cocktail and single phages were used to inactivate S. flexneri and V. cholerae, respectively. The cocktail was composed of three bacteriophages: φ SboM-AG3, φ SsoM-AG8, and φ SboM-AG10 (AG3, AG8, and AG10) specific for Shigella strains (Shigella boydii (C865-2), Shigella sonnei (C870-2), and S. flexneri (C869-2), respectively (Anany et al 2011)) at a final concentration of 10 10 PFU/mL. Single Vibrio phage JA-1 was provided by Dr. Richard Calendar (College of Science, University of California, Berkeley, CA, USA).…”

“…According to the models for optimal latent periods (Abedon et al 2001), food and bacterial viruses were 'kept in contact' for 90 min to ensure the phage adsorption and further host infection. Host ranges of the Shigella and Vibrio phages used have been analyzed by Anany et al (2011) and Fouts et al (2013), respectively, indicating respective infection of multiple strains of different Shigella genera and all tested strains of the V. cholerae serogroup O139.…”