A shift from papillary to reticular fibroblasts enables tumour–stroma interaction and invasion

Hogervorst, M.; Rietveld, Marion; Gruijl, Frank de; Ghalbzouri, Abdoelwaheb El

doi:10.1038/s41416-018-0024-y

Cited by 18 publications

(17 citation statements)

References 44 publications

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“…Epidermis and dermis were separated after removal of adipose tissue followed by enzymatic digestion and primary cell suspensions were obtained and cultured as described before [3,55]. Isolated primary keratinocytes and fibroblasts were tested for mycoplasma contamination using polymerase chain reaction (PCR) analysis before further use.…”

Section: Methodsmentioning

confidence: 99%

“…Sophisticated in vitro tools which resemble native human skin (NHS) to a high extent are three-dimensional human skin equivalents (HSEs). Therefore, these are widely applied in preclinical screenings and for research purposes to increase understanding of skin biology and epidermal barrier formation in healthy and diseased phenotypes [1,2,3,4,5,6]. HSEs mimic NHS in morphologic appearance, including the presence of distinguishable epidermal layers and formation of the SC [7,8,9].…”

Section: Introductionmentioning

confidence: 99%

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Contribution of Palmitic Acid to Epidermal Morphogenesis and Lipid Barrier Formation in Human Skin Equivalents

Mieremet

Helder

Nădăban

et al. 2019

IJMS

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The outermost barrier layer of the skin is the stratum corneum (SC), which consists of corneocytes embedded in a lipid matrix. Biosynthesis of barrier lipids occurs de novo in the epidermis or is performed with externally derived lipids. Hence, in vitro developed human skin equivalents (HSEs) are developed with culture medium that is supplemented with free fatty acids (FFAs). Nevertheless, the lipid barrier formation in HSEs remains altered compared to native human skin (NHS). The aim of this study is to decipher the role of medium supplemented saturated FFA palmitic acid (PA) on morphogenesis and lipid barrier formation in HSEs. Therefore, HSEs were developed with 100% (25 μM), 10%, or 1% PA. In HSEs supplemented with reduced PA level, the early differentiation was delayed and epidermal activation was increased. Nevertheless, a similar SC lipid composition in all HSEs was detected. Additionally, the lipid organization was comparable for lamellar and lateral organization, irrespective of PA concentration. As compared to NHS, the level of monounsaturated lipids was increased and the FFA to ceramide ratio was drastically reduced in HSEs. This study describes the crucial role of PA in epidermal morphogenesis and elucidates the role of PA in lipid barrier formation of HSEs.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Contribution of Palmitic Acid to Epidermal Morphogenesis and Lipid Barrier Formation in Human Skin Equivalents

Mieremet

Helder

Nădăban

et al. 2019

IJMS

Self Cite

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show abstract

“…the complement activation pathway (Stunova and Vistejnova, 2018). On the other hand, high expression of transglutaminase 2, calponin1, cadherin2, and matrix gla protein in Frs indicates that Frs express genes involved in cytoskeleton dynamics and cell motility (Shinde et al, 2017;Hogervorst et al, 2018). However, there also seem to be markers, such as CD26, that change dynamically with external factors.…”

Section: Molecular Phenotypementioning

confidence: 99%

Fibroblasts: Heterogeneous Cells With Potential in Regenerative Therapy for Scarless Wound Healing

Zou

Teng

et al. 2021

Front. Cell Dev. Biol.

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In recent years, research on wound healing has become increasingly in-depth, but therapeutic effects are still not satisfactory. Occasionally, pathological tissue repair occurs. Influencing factors have been proposed, but finding the turning point between normal and pathological tissue repair is difficult. Therefore, we focused our attention on the most basic level of tissue repair: fibroblasts. Fibroblasts were once considered terminally differentiated cells that represent a single cell type, and their heterogeneity was not studied until recently. We believe that subpopulations of fibroblasts play different roles in tissue repair, resulting in different repair results, such as the formation of normal scars in physiological tissue repair and fibrosis or ulcers in pathological tissue repair. It is also proposed that scarless healing can be achieved by regulating fibroblast subpopulations.

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“…To date there have been few studies of how different fibroblast lineages contribute to tumour stroma formation, and whether the tumour stroma differs between different types of skin cancers. Interestingly, one study showed that fibroblasts of the reticular dermis are predisposed to differentiate into CAFs upon cSCC signals, assisting invasion and EMT [60].…”

Section: Skin Cancermentioning

confidence: 99%

The Roles of YAP/TAZ and the Hippo Pathway in Healthy and Diseased Skin

Rognoni

Walko

2019

Cells

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Skin is the largest organ of the human body. Its architecture and physiological functions depend on diverse populations of epidermal cells and dermal fibroblasts. Reciprocal communication between the epidermis and dermis plays a key role in skin development, homeostasis and repair. While several stem cell populations have been identified in the epidermis with distinct locations and functions, there is additional heterogeneity within the mesenchymal cells of the dermis. Here, we discuss the current knowledge of how the Hippo pathway and its downstream effectors Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) contribute to the maintenance, activation and coordination of the epidermal and dermal cell populations during development, homeostasis, wound healing and cancer.

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A shift from papillary to reticular fibroblasts enables tumour–stroma interaction and invasion

Abstract: Conversely, our results show that Pfs contain cancer cells more within the epidermis. Rfs are clearly predisposed to differentiate into CAFs upon SCC signals, assisting invasion and EMT.

Cited by 18 publications

References 44 publications

Contribution of Palmitic Acid to Epidermal Morphogenesis and Lipid Barrier Formation in Human Skin Equivalents

Contribution of Palmitic Acid to Epidermal Morphogenesis and Lipid Barrier Formation in Human Skin Equivalents

Fibroblasts: Heterogeneous Cells With Potential in Regenerative Therapy for Scarless Wound Healing

The Roles of YAP/TAZ and the Hippo Pathway in Healthy and Diseased Skin

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