A shadow image microscope based on an array of nanoLEDs

Canals, Joan; Moro, Victor; Franch, Nil; Moreno, Sergio Ruiz; Alonso, Oscar; Vilà, A.; Prades, Joan Daniel; Gülink, Jan; Bezshlyakh, Daria; Waag, A.; Diéguez, Ángel

doi:10.1117/12.2559394

Cited by 3 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the research presented in this work paves the way for the development of miniaturized microscopes [ 80 , 81 , 82 , 83 , 84 ] based on fluorescence, the gold standard tool used in biology. This promising advancement is envisaged through the utilization of large arrays integrating smaller LEDs, complemented by the appropriate driving circuits.…”

Section: Discussionmentioning

confidence: 99%

Fluorescence Multi-Detection Device Using a Lensless Matrix Addressable microLED Array

Moro,

Canals,

Moreno

et al. 2024

Biosensors

View full text Add to dashboard Cite

A Point-of-Care system for molecular diagnosis (PoC-MD) is described, combining GaN and CMOS chips. The device is a micro-system for fluorescence measurements, capable of analyzing both intensity and lifetime. It consists of a hybrid micro-structure based on a 32 × 32 matrix addressable GaN microLED array, with square LEDs of 50 µm edge length and 100 µm pitch, with an underneath wire bonded custom chip integrating their drivers and placed face-to-face to an array of 16x16 single-photon avalanche diodes (SPADs) CMOS. This approach replaces instrumentation based on lasers, bulky optical components, and discrete electronics with a full hybrid micro-system, enabling measurements on 32 × 32 spots. The reported system is suitable for long lifetime (>10 ns) fluorophores with a limit of detection ~1/4 µM. Proof-of-concept measurements of streptavidin conjugate Qdot™ 605 and Amino PEG Qdot™ 705 are demonstrated, along with the device ability to detect both fluorophores in the same measurement.

show abstract

Section: Discussionmentioning

confidence: 99%

Fluorescence Multi-Detection Device Using a Lensless Matrix Addressable microLED Array

Moro,

Canals,

Moreno

et al. 2024

Biosensors

View full text Add to dashboard Cite

show abstract

“…First, the camera was fixed over the LED array in the distances necessary for the measurement, but without a sample inserted. Next, the sensing area (size and position) and the target intensity were selected, taking care to avoid saturating the sensor and using a small sensing area centered with the LED array, since they had a direct impact on the contrast of the reconstructed image [ 24 ]. Once the camera position and all user-defined parameters were set, each LED was individually turned on, and its intensity was measured and equalized by varying the duty cycle of the PWM driving signal until it matched the target intensity.…”

Section: Methodsmentioning

confidence: 99%

A Novel Approach for a Chip-Sized Scanning Optical Microscope

et al. 2021

Self Cite

View full text Add to dashboard Cite

The recent advances in chip-size microscopy based on optical scanning with spatially resolved nano-illumination light sources are presented. This new straightforward technique takes advantage of the currently achieved miniaturization of LEDs in fully addressable arrays. These nano-LEDs are used to scan the sample with a resolution comparable to the LED sizes, giving rise to chip-sized scanning optical microscopes without mechanical parts or optical accessories. The operation principle and the potential of this new kind of microscope are analyzed through three different implementations of decreasing LED dimensions from 20 µm down to 200 nm.

show abstract

“…This paper focuses on the fabrication of nanoLED arrays with single pixel control as key elements for future chipbased super-resolution microscopes, followed by a thorough analysis of the optical and electrical properties. Details on the performance as a chip microscope can be found in related publications 13,14 .…”

Section: Introductionmentioning

confidence: 99%

Directly addressable GaN-based nano-LED arrays: fabrication and electro-optical characterization

et al. 2020

Self Cite

View full text Add to dashboard Cite

The rapid development of display technologies has raised interest in arrays of self-emitting, individually controlled light sources atthe microscale. Gallium nitride (GaN) micro-light-emitting diode (LED) technology meets this demand. However, the current technology is not suitable for the fabrication of arrays of submicron light sources that can be controlled individually. Our approach is based on nanoLED arrays that can directly address each array element and a self-pitch with dimensions below the wavelength of light. The design and fabrication processes are explained in detail and possess two geometries: a 6 × 6 array with 400 nm LEDs and a 2 × 32 line array with 200 nm LEDs. These nanoLEDs are developed as core elements of a novel on-chip super-resolution microscope. GaN technology, based on its physical properties, is an ideal platform for such nanoLEDs.

show abstract

A shadow image microscope based on an array of nanoLEDs

Cited by 3 publications

References 0 publications

Fluorescence Multi-Detection Device Using a Lensless Matrix Addressable microLED Array

Fluorescence Multi-Detection Device Using a Lensless Matrix Addressable microLED Array

A Novel Approach for a Chip-Sized Scanning Optical Microscope

Directly addressable GaN-based nano-LED arrays: fabrication and electro-optical characterization

Contact Info

Product

Resources

About