A set of microsatellite primers for the striped possum, <i>Dactylopsila trivirgata</i> (Petauridae: Marsupialia)

Hansen, Birgita D.; French, Jordan; Handasyde, Kathrine A.; Kendal, T A; Taylor, Andrea Carolyn

doi:10.1046/j.1471-8286.2003.00401.x

Cited by 3 publications

(3 citation statements)

References 11 publications

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Section: Methodsmentioning

confidence: 99%

“…All samples were genotyped using 14 polymorphic microsatellite markers developed for Gymnobelideus leadbeateri (GL4, GL5A, GL6, GL7, GL13, GL19B, GL24, GL28, GL33, GL35, GL38, GL39, GL42, GL44; Hansen et al 2005) and one from another petaurid, the striped possum, Dactylopsila trivirgata (DT1; Hansen et al 2003). Primer sequences and polymerase chain reaction (PCR) conditions for amplification of microsatellite markers are described in Hansen et al (2005) except for DT1, which was amplified using touchdown cycling from 62 to 55°C.…”

Section: Sample Collection and Microsatellite Genotypingmentioning

confidence: 99%

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Isolated remnant or recent introduction? Estimating the provenance of Yellingbo Leadbeater's possums by genetic analysis and bottleneck simulation

Hansen

Taylor

2008

Molecular Ecology

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Effective conservation management requires that genetically divergent populations potentially harbouring important local adaptations be identified and maintained as separate management units. In the case of the endangered Australian Leadbeater's possum (Gymnobelideus leadbeateri), an arboreal marsupial endemic to Victoria, uncertainty over the evolutionary origin of a potentially important extant wild population recently discovered in atypical habitat (lowland swamp) at Yellingbo is hampering such efforts. The population is rumoured to be a recent introduction. Microsatellite allele frequencies at Yellingbo differed substantially from those in sampled populations in montane ash forest (F(ST) between 0.23 and 0.36), and Bayesian clustering analyses of genotypes strongly separated them (K = 2). We conducted a suite of bottlenecking tests which all indicated that Yellingbo had undergone a recent reduction in size. The extent to which the distinctiveness of Yellingbo animals might be expected solely through bottlenecking associated with a recent introduction, was tested by simulating population-history scenarios seeded with genotypes from candidate wild and captive sources. No bottleneck scenario reproduced anything approaching the genetic distinction of the Yellingbo population, with all STRUCTURE analyses placing Yellingbo in a separate cluster to simulated populations (K = 2, minimum F(ST) = 0.13). These results suggest that Yellingbo does not share recent ancestry with other extant populations and instead may be a remnant of an otherwise extinct gene pool. Importantly, this may include genes involved in adaptation to a lowland swamp environment, substantially adding to the conservation importance of this population, and suggesting that separate management may be prudent until evidence suggests otherwise.

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Section: Methodsmentioning

confidence: 99%

Section: Sample Collection and Microsatellite Genotypingmentioning

confidence: 99%

Isolated remnant or recent introduction? Estimating the provenance of Yellingbo Leadbeater's possums by genetic analysis and bottleneck simulation

Hansen

Taylor

2008

Molecular Ecology

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“…Amplification of these loci in four other petaurid species (the striped possum Dactylopsila trivirgata , the sugar glider Petaurus breviceps , the squirrel glider Petaurus norfolcensis and the mahogany glider Petaurus gracilis ) was not very successful and little polymorphism was evident (see Table 2). This is not unexpected given that G. leadbeateri is thought to have diverged from other petaurids as long as 32 Ma, and that other available petaurid primers hybridize to only a limited degree in G. leadbeateri (Edwards & Westerman 1992; Hansen et al . 2003).…”

Section: Details Of the 17 Leadbeater's Possum Microsatellite Loci ‘mentioning

confidence: 89%

A set of microsatellite markers for an endangered arboreal marsupial, Leadbeater's possum

Hansen

Sunnucks

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et al. 2005

Molecular Ecology Notes

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Leadbeater's possum is a small, cryptic nongliding petaurid, which is threatened by habitat loss due to the collapse of large hollow‐bearing trees and ongoing timber production in Victoria's central highlands. The recent decline of this species and the difficulties faced by researchers collecting field data has prompted the development of a suite of polymorphic microsatellite markers for application to the continuing conservation of the Leadbeater's possum.

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Marsupial Population and Conservation Genetics

Eldridge

2010

Marsupial Genetics and Genomics

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Recent technological advances have resulted in a dramatic renaissance of population genetics and its application to species ecology and conservation. This review summarizes the progress made in applying these new techniques, notably hypervariable genetic markers (microsatellite loci and mitochondrial control region), to the study of marsupials. Since 1990, population genetic studies have overwhelmingly been of marsupial species from eastern and southern Australia, largely focusing on threatened species or those with restricted distributions. To date, over 500 polymorphic microsatellite loci have been isolated from 38 marsupial species (six American, 32 Australian), including representatives from 13 of the 18 extant marsupial families. Levels of microsatellite diversity identified within the 209 marsupial populations (43 species) so far examined have varied greatly, although the range is similar to those reported from eutherians and other vertebrates. Marsupial populations with high levels of genetic diversity tend to be those from relatively abundant or widespread species, while those with lower levels are typically species with restricted distributions, that are threatened, found on islands or have been established via translocation. Although data for most families are still limited, bandicoots, koalas and wombats appear less and phalangerids more diverse than the marsupial average. The application of these hypervariable genetic markers to investigate marsupial species ecology has substantially improved our understanding of population biology, behaviour and reproduction in many species, as well as informing conservation initiatives and management plans for many threatened marsupial taxa. It is hoped that in the future, marsupial population genetic studies can be expanded to include a larger number of South American and New Guinean species, as well as a better representation of arid, tropical, widespread and abundant Australian species.

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A set of microsatellite primers for the striped possum, Dactylopsila trivirgata (Petauridae: Marsupialia)

Cited by 3 publications

References 11 publications

Isolated remnant or recent introduction? Estimating the provenance of Yellingbo Leadbeater's possums by genetic analysis and bottleneck simulation

Isolated remnant or recent introduction? Estimating the provenance of Yellingbo Leadbeater's possums by genetic analysis and bottleneck simulation

A set of microsatellite markers for an endangered arboreal marsupial, Leadbeater's possum

Marsupial Population and Conservation Genetics

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