Abstract:We present a set of 35 chloroplast microsatellite primers for Eucalyptus. Ten of the microsatellites displayed intraspecific polymorphism, and identified nine haplotypes among 16 Eucalyptus globulus individuals. Primer conservation was high, with a polymerase chain reaction (PCR) success rate of 98% when tested on four other Eucalyptus species and seven additional myrtaceous genera. Chloroplast microsatellites have applications in phylogeographic studies, fingerprinting and progeny analysis.
“…Chloroplast microsatellites were amplified with primers designed for Eucalyptus species (Steane et al. , 2005).…”
Section: Methodsmentioning
confidence: 99%
“…Chloroplast microsatellites were amplified with primers designed for Eucalyptus species (Steane et al, 2005). Chloroplast DNA is maternally inherited in most angiosperms, including eucalypts (Byrne et al, 1993;McKinnon et al, 2001b).…”
“…In our study we used cpDNA microsatellites (cpSSR) developed for eucalypt species (Steane et al. , 2005) to examine chloroplast haplotype diversity and structure for 41 populations of E. regnans.…”
Section: Introductionmentioning
confidence: 99%
“…In our study we used cpDNA microsatellites (cpSSR) developed for eucalypt species (Steane et al, 2005) to examine chloroplast haplotype diversity and structure for 41 populations of E. regnans. Specifically, this work aimed to: (1) identify any major evolutionary divergences or disjunctions across the species' range; and (2) examine signatures of refugia and expansion to infer historical migration and colonization events for montane species in south-eastern Australia.…”
Aim There is a need for more Southern Hemisphere phylogeography studies, particularly in Australia, where, unlike much of Europe and North America, ice sheet cover was not extensive during the Last Glacial Maximum (LGM). This study examines the phylogeography of the south-east Australian montane tree species Eucalyptus regnans. The work aimed to identify any major evolutionary divergences or disjunctions across the species' range and to examine genetic signatures of past range contraction and expansion events.Location South-eastern mainland Australia and the large island of Tasmania.Methods We determined the chloroplast DNA haplotypes of 410 E. regnans individuals (41 locations) based on five chloroplast microsatellites. Genetic structure was examined using analysis of molecular variance (AMOVA), and a statistical parsimony tree was constructed showing the number of nucleotide differences between haplotypes. Geographic structure in population genetic diversity was examined with the calculation of diversity parameters for the mainland and Tasmania, and for 10 regions. Regional analysis was conducted to test hypotheses that some areas within the species' current distribution were refugia during the LGM and that other areas have been recolonized by E. regnans since the LGM.Results Among the 410 E. regnans individuals analysed, 31 haplotypes were identified. The statistical parsimony tree shows that haplotypes divided into two distinct groups corresponding to mainland Australia and Tasmania. The distribution of haplotypes across the range of E. regnans shows strong geographic patterns, with many populations and even certain regions in which a particular haplotype is fixed. Many locations had unique haplotypes, particularly those in East Gippsland in south-eastern mainland Australia, north-eastern Tasmania and south-eastern Tasmania. Higher haplotype diversity was found in putative refugia, and lower haplotype diversity in areas likely to have been recolonized since the LGM.
Main conclusionsThe data are consistent with the long-term persistence of E. regnans in many regions and the recent recolonization of other regions, such as the Central Highlands of south-eastern mainland Australia. This suggests that, in spite of the narrow ecological tolerances of the species and the harsh environmental conditions during the LGM, E. regnans was able to persist locally or contracted to many near-coastal refugia, maintaining a diverse genetic structure.
“…Chloroplast microsatellites were amplified with primers designed for Eucalyptus species (Steane et al. , 2005).…”
Section: Methodsmentioning
confidence: 99%
“…Chloroplast microsatellites were amplified with primers designed for Eucalyptus species (Steane et al, 2005). Chloroplast DNA is maternally inherited in most angiosperms, including eucalypts (Byrne et al, 1993;McKinnon et al, 2001b).…”
“…In our study we used cpDNA microsatellites (cpSSR) developed for eucalypt species (Steane et al. , 2005) to examine chloroplast haplotype diversity and structure for 41 populations of E. regnans.…”
Section: Introductionmentioning
confidence: 99%
“…In our study we used cpDNA microsatellites (cpSSR) developed for eucalypt species (Steane et al, 2005) to examine chloroplast haplotype diversity and structure for 41 populations of E. regnans. Specifically, this work aimed to: (1) identify any major evolutionary divergences or disjunctions across the species' range; and (2) examine signatures of refugia and expansion to infer historical migration and colonization events for montane species in south-eastern Australia.…”
Aim There is a need for more Southern Hemisphere phylogeography studies, particularly in Australia, where, unlike much of Europe and North America, ice sheet cover was not extensive during the Last Glacial Maximum (LGM). This study examines the phylogeography of the south-east Australian montane tree species Eucalyptus regnans. The work aimed to identify any major evolutionary divergences or disjunctions across the species' range and to examine genetic signatures of past range contraction and expansion events.Location South-eastern mainland Australia and the large island of Tasmania.Methods We determined the chloroplast DNA haplotypes of 410 E. regnans individuals (41 locations) based on five chloroplast microsatellites. Genetic structure was examined using analysis of molecular variance (AMOVA), and a statistical parsimony tree was constructed showing the number of nucleotide differences between haplotypes. Geographic structure in population genetic diversity was examined with the calculation of diversity parameters for the mainland and Tasmania, and for 10 regions. Regional analysis was conducted to test hypotheses that some areas within the species' current distribution were refugia during the LGM and that other areas have been recolonized by E. regnans since the LGM.Results Among the 410 E. regnans individuals analysed, 31 haplotypes were identified. The statistical parsimony tree shows that haplotypes divided into two distinct groups corresponding to mainland Australia and Tasmania. The distribution of haplotypes across the range of E. regnans shows strong geographic patterns, with many populations and even certain regions in which a particular haplotype is fixed. Many locations had unique haplotypes, particularly those in East Gippsland in south-eastern mainland Australia, north-eastern Tasmania and south-eastern Tasmania. Higher haplotype diversity was found in putative refugia, and lower haplotype diversity in areas likely to have been recolonized since the LGM.
Main conclusionsThe data are consistent with the long-term persistence of E. regnans in many regions and the recent recolonization of other regions, such as the Central Highlands of south-eastern mainland Australia. This suggests that, in spite of the narrow ecological tolerances of the species and the harsh environmental conditions during the LGM, E. regnans was able to persist locally or contracted to many near-coastal refugia, maintaining a diverse genetic structure.
“…We initially screened eight individuals of E. serraensis and E. verrucata for amplification and polymorphisms with 10 chloroplast DNA microsatellite primer pairs developed by Steane et al (2005): EMCRC59cp, EMCRC60cp, EMCRC62cp, EMCRC65cp, EMCRC67cp, EMCRC74cp, EMCRC84cp, EM-CRC85cp, EMCRC86cp and EMCRC90cp. All primer pairs amplified a product of the expected size range and four were polymorphic (EMCRC59cp, EMCRC60cp, EMCRC67cp and EMCRC86cp).…”
Section: Amplification and Screening Of Chloroplast Microsatellitesmentioning
Aim To relate genetic diversity to topographic features and to investigate genetic interactions between Eucalyptus species in a local centre of endemism and diversity in south‐eastern Australia.
Location Grampian Ranges, Victoria, Australia.
Methods We documented chloroplast DNA (cpDNA) variation for a group of endemic Eucalyptus species (E. serraensis, E. verrucata and E. victoriana) that dominate rocky, high‐elevation ridgelines of the Grampian Ranges and for one closely‐related, widespread species (E. baxteri) occupying flanking slopes and valleys. We documented genetic patterns across the landscape using cpDNA microsatellites, and related them to topographic features (exposed west‐facing versus protected east‐facing slopes and valleys). We also determined the extent of local haplotype sharing between populations of endemic species and neighbouring E. baxteri downslope with cpDNA microsatellites, and haplotype sharing between the endemic group and more distantly related species (E. obliqua, E. pauciflora and E. willisii) with sequences of the JLA+ chloroplast region.
Results We detected 26 cpDNA microsatellite haplotypes in a relatively small area of c. 20 km × 50 km. Populations of E. baxteri on east‐facing slopes and valleys had greater cpDNA microsatellite diversity than E. baxteri and endemic species on exposed west‐facing slopes. Endemic species frequently shared chloroplast haplotypes with E. baxteri downslope. Sharing of JLA+ haplotypes with species outside the endemic group was mostly restricted to E. victoriana, which had cpDNA more similar to the species from other sections of Eucalyptus (E. obliqua, E. willisii and E. pauciflora).
Main conclusions Intensive sampling of related species on small isolated mountain ranges allowed us to relate genetic diversity to fine‐scale habitats and to document extensive local haplotype sharing between species. This study contributes to a general understanding of the environmental conditions that enable plant population persistence by linking concentrations of genetic diversity to particular habitats.
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