A SERS-based competitive immunoassay for highly sensitive and specific detection of ochratoxin A

Ding, Yangjun; Shang, Hezhen; Wang, Xiaokun; Chen, Lingxin

doi:10.1039/d0an01220c

Cited by 35 publications

(12 citation statements)

References 39 publications

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“…The intensities of 1456 cm −1 peak compared with the acrylamide concentrations were curve fitted and the best fitting (R 2 = 0.995) was found to be the second order polynomial fit (Figure 9) with the equation y = 3.01 + 20.02x – 1.98x 2 . For calculating the limit of detection (LOD), the standard IUPAC method was used with Equation () 49–52

italicLOD goodbreak= {\overset{true¯}{y}}_{b} goodbreak+ t_{α}^{n - 1} goodbreak\times s_{b}^{n - 1} \sqrt{1 + \frac{1}{n}}

where

{\overset{true¯}{y}}_{b}

is the average blank signal value,

t_{α}^{n - 1}

is the critical t value of one‐sided t‐distribution test (with a confidence level of 95%),

s_{b}^{n - 1}

is the standard deviation with

n - 1

degrees of freedom, and n is the number of replications.…”

Section: Resultsmentioning

confidence: 99%

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A disposable ultrasensitive surface enhanced Raman spectroscopy biosensor platform fabricated from biodegradable zein nanofibers

Turasan

Çakmak

Kokini

2022

J of Applied Polymer Sci

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In this paper, a natural polymer, zein, is converted into a disposable and environmentally friendly surface enhanced Raman spectroscopy (SERS) biosensor platform to detect toxins. First, protein nanofiber mats were fabricated using electrospinning. Next, the surface of the fibers was decorated with metallic nanoparticles to create hotspots for the detection of the target molecule. Four types of metallic nanoparticles were tested for sensitivity optimization: gold, silver, silver‐shelled‐gold, and a mixture of gold and silver nanoparticles. Silver‐shelled‐gold nanoparticles gave the highest SERS intensity, and at the concentration of 1012 particles/ml, the highest enhancement factor (EF) of 2.49 × 106 was reached, which is the highest EF ever reported for a zein‐based biodegradable platform. Acrylamide was used as the model food carcinogenic toxin and its detection with this nanofiber‐based platform gave a limit of detection of 2.06 ng/ml. This LOD is 104 times lower than the limit of detection that was achieved previously with a zein‐based SERS platform. Other sensitive analytical methods (GC–MS and LC–MS/MS) gave LODs of 5–10 and 20–50 ng/ml for acrylamide, much higher than the method used in this study. This sensitivity is much lower than any other previous attempts to detect acrylamide with biodegradable sensors.

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italicLOD goodbreak= {\overset{true¯}{y}}_{b} goodbreak+ t_{α}^{n - 1} goodbreak\times s_{b}^{n - 1} \sqrt{1 + \frac{1}{n}}

where

{\overset{true¯}{y}}_{b}

is the average blank signal value,

t_{α}^{n - 1}

is the critical t value of one‐sided t‐distribution test (with a confidence level of 95%),

s_{b}^{n - 1}

is the standard deviation with

n - 1

degrees of freedom, and n is the number of replications.…”

Section: Resultsmentioning

confidence: 99%

“…For calculating the limit of detection (LOD), the standard IUPAC method was used with Equation (2). [49][50][51][52] F I G U R E 7 Raman measurement of 0.…”

Section: Effect Of Nanoparticle Concentration On Sers Enhancement Of ...mentioning

confidence: 99%

A disposable ultrasensitive surface enhanced Raman spectroscopy biosensor platform fabricated from biodegradable zein nanofibers

Turasan

Çakmak

Kokini

2022

J of Applied Polymer Sci

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“…5 The pressing need for innovative methods to detect multiple contaminations in both environment and food is clear, and the research on new analytical sorbents and techniques to use in this eld is crucial. [6][7][8][9] Triple-quadrupole mass spectrometers coupled with liquid chromatography (LC) are commonly used in pesticide residue analyses, employing the multiple reaction monitoring (MRM) mode for enhanced specicity. However, the capability to simultaneously monitor compounds is constrained by dwell time, limiting peak data acquisition.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and evaluation of a chitosan nanomaterial as efficient sorbent for determination of fungicide residues in waters and wine by liquid chromatography high resolution mass spectrometry

Martello,

Rapti,

Bikiaris

et al. 2024

Anal. Methods

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“…However, the detection time was long, and the pathogenic bacteria were lethal in the process of processing and could not be detected. Besides, several electrochemical biosensors, chemiluminescence assay, surface-enhanced Raman scattering (SERS) assay, and fluorescence assay were also developed to detect SEs. − Alternatively, SERS biosensors possessed high sensitivity down to single-molecule detection and showed potential for application in biological molecule analysis, − environmental monitoring, , and disease diagnosis, − and would be a promising tool for reliable SE detection. However, there were still some issues to be solved for SERS signal-dependent sensitive and accurate SE detection: (1) the high and non-interference SERS signals for ultrasensitive and precise detection; (2) the uniform position of Raman labels on the surface of SERS for the production of reproducible SERS signals.…”

Section: Introductionmentioning

confidence: 99%

SERS-Active Composites with Au–Ag Janus Nanoparticles/Perovskite in Immunoassays for Staphylococcus aureus Enterotoxins

Shi

Jing

et al. 2022

ACS Appl. Mater. Interfaces

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The accurate detection of Staphylococcus aureus enterotoxins (SEs) is vital for food safety owing to their high pathogenicity, which may be performed with surface-enhanced Raman scattering (SERS) if SERS-active nanostructures are used. Herein, a Au−Ag Janus nanoparticle (NPs)/perovskite compositeengineered SERS immunoassay was developed for SEC detection. Plasmonic Au−Ag Janus NPs demonstrated inherent SERS activity from the 2-mercaptobenzoimidazole-5-carboxylic acid ligands. CsPbBr 3 @mesoporous silica nanomaterials (MSNs) were prepared and transformed into CsPb 2 Br 5 @MSNs in the aqueous phase. Paired SEC antibody−antigen-driven plasmonic Au−Ag Janus NP-CsPb 2 Br 5 @MSN composites were prepared. They showed amplified SERS activity, attributed to the depressed plasmonic decay due to electromagnetic field enhancement and the electron transfer mechanism. A positive relationship was established between SERS signals of composites and the SEC concentration. An additive-free SERS immunoassay was developed for simple, sensitive, and reproducible SEC detection. This study will be extended to develop multiple additive-free SERS-active plasmonic NP/ perovskite composites that will open up the possibility of exploring more SERS detection probes for food safety monitoring.

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A SERS-based competitive immunoassay for highly sensitive and specific detection of ochratoxin A

Abstract: Ochratoxin A (OTA) is a well-known carcinogenic contaminant in food commodities. Highly sensitive and specific detection of OTA level is an essential way to prevent health risk of human/animal. In...

Cited by 35 publications

References 39 publications

A disposable ultrasensitive surface enhanced Raman spectroscopy biosensor platform fabricated from biodegradable zein nanofibers

A disposable ultrasensitive surface enhanced Raman spectroscopy biosensor platform fabricated from biodegradable zein nanofibers

Synthesis and evaluation of a chitosan nanomaterial as efficient sorbent for determination of fungicide residues in waters and wine by liquid chromatography high resolution mass spectrometry

SERS-Active Composites with Au–Ag Janus Nanoparticles/Perovskite in Immunoassays for Staphylococcus aureus Enterotoxins

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