A SERS aptasensor for sensitive and selective detection of bis(2-ethylhexyl)phthalate

Tu, Dandan; Garza, Javier T.; Coté, Gerard L.

doi:10.1039/c8ra09230c

Cited by 36 publications

(19 citation statements)

References 56 publications

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“…Tu et al designed a competitive binding assay for DEHP determination by aptamer modified MNPs and SERS silica particles [60]. On the one hand, the MNPs were functionalized with the DEHP aptamers by streptavidin–biotin interaction.…”

Section: Aptamer-based Sers Probes In Quantitative Analysis and Bimentioning

confidence: 99%

Development and Application of Aptamer-Based Surface-Enhanced Raman Spectroscopy Sensors in Quantitative Analysis and Biotherapy

Wang

Zhao

et al. 2019

Sensors

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Surface-enhanced Raman scattering (SERS) is one of the most special and important Raman techniques. An apparent Raman signal can be observed when the target molecules are absorbed onto the surface of the SERS substrates, especially on the “hot spots” of the substrates. Early research focused on exploring the highly active SERS substrates and their detection applications in label-free SERS technology. However, it is a great challenge to use these label-free SERS sensors for detecting hydrophobic or non-polar molecules, especially in complex systems or at low concentrations. Therefore, antibodies, aptamers, and antimicrobial peptides have been used to effectively improve the target selectivity and meet the analysis requirements. Among these selective elements, aptamers are easy to use for synthesis and modifications, and their stability, affinity and specificity are extremely good; they have been successfully used in a variety of testing areas. The combination of SERS detection technology and aptamer recognition ability not only improved the selection accuracy of target molecules, but also improved the sensitivity of the analysis. Variations of aptamer-based SERS sensors have been developed and have achieved satisfactory results in the analysis of small molecules, pathogenic microorganism, mycotoxins, tumor marker and other functional molecules, as well as in successful photothermal therapy of tumors. Herein, we present the latest advances of the aptamer-based SERS sensors, as well as the assembling sensing platforms and the strategies for signal amplification. Furthermore, the existing problems and potential trends of the aptamer-based SERS sensors are discussed.

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Section: Aptamer-based Sers Probes In Quantitative Analysis and Bimentioning

confidence: 99%

Development and Application of Aptamer-Based Surface-Enhanced Raman Spectroscopy Sensors in Quantitative Analysis and Biotherapy

Wang

Zhao

et al. 2019

Sensors

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“…The resulting structure presents high selectivity for detection of malation in mixed solution of pesticides . The coating of cluster of Raman reporter molecules by silica favors the development or more stable SERS systems …”

Section: Theoretical Backgroundmentioning

confidence: 99%

“…43 The coating of cluster of Raman reporter molecules by silica favors the development or more stable SERS systems. 44 In addition to the modification of plasmonic centers, other important parameter to be considered in production of device refers to the substrate nature. 45…”

Section: Sers Mechanisms Sensors Selectivity and Applicationsmentioning

confidence: 99%

Silver‐based surface enhanced Raman spectroscopy devices for detection of organophosphorus pesticides traces

Soares

2019

Biotechnology Progress

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The detection of traces of substances by surface‐sensitive techniques such as surface enhanced Raman spectroscopy (SERS) explores the interaction of adsorbed molecules on plasmonic surfaces to improve the limit of detection of analytes. This article is an overview about recent development in SERS substrates applied in the detection of organophosphorus pesticides on plasmonic surfaces (arrays of metal nanoparticles). The morphology, roughness, chemical functionalization degree, and aggregation level of plasmonic centers are some of the critical parameters to be controlled in the optimization of SERS signal from specific analytes.

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“…However, these methods are expensive, time-consuming, and have high LOD, which inhibit their application in the field for on-spot rapid detection. Alternatively, enzyme-linked immunosorbent assay (ELISA) has a LOD of 4.2 pg/mL [11], while the LOD is 3.9 pg/mL [12] for electrochemical aptasensor, 0.5 pg/mL for quantum dot aptasensor [13], and 8 pM for surface-enhanced raman spectroscopy (SERS) aptasensor [14]. The obvious advantages of these rapid-detection techniques are that they are fast, low-cost, and exhibit high selectivity and sensitivity in monitoring trace DEHP in samples.…”

mentioning

confidence: 99%

“…However, this detection technology was complex and was thus not used to detect real samples in their research. An aptasensor [14] based on SERS has also been developed with a LOD of 8 pM. However, this method included a series of procedures involving magnetic particles and silver nanoparticles with silica.…”

mentioning

confidence: 99%

Selection of Aptamers Specific for DEHP Based on ssDNA Library Immobilized SELEX and Development of Electrochemical Impedance Spectroscopy Aptasensor

et al. 2020

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A selection of aptamers specific for di(2-ethylhexyl) phthalate (DEHP) and development of electrochemical impedance spectroscopy (EIS) aptasensor are described in this paper. The aptamers were selected from an immobilized ssDNA library using the systematic evolution of ligands by exponential enrichment (SELEX). The enrichment was monitored using real-time quantitative PCR (Q-PCR), and the aptamers were identified by high-throughput sequencing (HTS), gold nanoparticles (AuNPs) colorimetric assay, and localized surface plasmon resonance (LSPR). The EIS aptasensor was developed to detect DEHP in water samples. After eight rounds of enrichment, HTS, AuNPs colorimetric assay, and LSPR analysis indicated that four aptamers had higher binding activity, and aptamer 31 had the highest affinity (Kd = 2.26 ± 0.06 nM). The EIS aptasensor had a limit of detection (LOD) of 0.103 pg/mL with no cross-reactivity to DEHP analogs and a mean recovery of 76.07% to 141.32% for detection of DEHP in water samples. This aptamer is novel with the highest affinity and sensitivity.

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A SERS aptasensor for sensitive and selective detection of bis(2-ethylhexyl)phthalate

Abstract: This paper reports a SERS aptasensor developed to detect DEHP at relevant ranges with ultrasensitive performance and good selectivity.

Cited by 36 publications

References 56 publications

Development and Application of Aptamer-Based Surface-Enhanced Raman Spectroscopy Sensors in Quantitative Analysis and Biotherapy

Development and Application of Aptamer-Based Surface-Enhanced Raman Spectroscopy Sensors in Quantitative Analysis and Biotherapy

Silver‐based surface enhanced Raman spectroscopy devices for detection of organophosphorus pesticides traces

Selection of Aptamers Specific for DEHP Based on ssDNA Library Immobilized SELEX and Development of Electrochemical Impedance Spectroscopy Aptasensor

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