A serologically detected tumour-specific membrane antigen of murine lymphomas which is not the target for syngeneic graft rejection

Purification of immunoprotective tumor antigens by preparative isotachophoresis

“…These antisera were functionally specific for MCA-F tumor cells as assessed by an indirect membrane immunofluorescence assay [34]. Antisera were produced in a xenogeneic host because of the difficulty in generating antibodies to TSTA in the syngeneic host [7,9,16]. Altoantisera were prepared by transplanting MCA-F tumor cells into mutant C3HfeB/HeN mice.…”

“…Xenoantisera to TSTA were used because of the difficulties in producing anti-TSTA antibody in syngeneic [7,16] and allogeneic hosts [9]. We [34] and others [4,33] have shown that xenoimmunization with purified tumor antigens provides specific antisera which do not require extensive absorption to demonstrate specificity.…”

Immunopathology

“…However, their meaning will not be plain until the cell surface studies are repeated with carefully separated subpopulation of preleukemic thymic cells. Finally, the thymus may serve as a privileged site for viral replication, especially since Davey et al (1979) found that a tumor-associated cross-reacting cell surface antigen of a DBA/2 mouse lymphoma did not serve as target for syngeneic graft rejection.…”

Identification of a unique tumor antigen as rejection antigen by molecular cloning and gene transfer.

“…This finding of unique tumor specificity suggested that the diversity of these tumor-specific antigens is very large .Identifying the molecular nature of these unique tumor-specific antigens that cause tumor rejection has proven to be extremely difficult in the past . Serological probes with unique tumor specificity are difficult to obtain (6, 7), and the serologically recognized antigens may not be the target for tumor rejection (8,9) that is primarily T cell-mediated (10).Like other neoplasias, U V light-induced tumors display unique tumor-specific transplantation antigens (11,12), and we used such tumors for studying the nature of these antigens for the following reasons: (a) the unique tumor-specific rejection antigens on UV-induced tumors are stronger than those on chemically induced tumors in that UV-induced tumors often regress after transplantation into normal mice even without prior immunization (13); (b) several of the tumorspecific rejection antigens on one such UV-induced regressor (RE) tumor, called 1591-RE, have been defined by cytolytic T cell clones (14-16); (c) Philipps et al . (17) have generated mAbs with unique specificity for this UV-induced RE tumor that reacted with novel MHC class I molecules on this tumor; and (d) the genes encoding these novel class I molecules have been cloned (18), identified by transfection (18), and their DNA sequence has been determined (19).…”

“…Identifying the molecular nature of these unique tumor-specific antigens that cause tumor rejection has proven to be extremely difficult in the past . Serological probes with unique tumor specificity are difficult to obtain (6, 7), and the serologically recognized antigens may not be the target for tumor rejection (8,9) that is primarily T cell-mediated (10).…”