A serological follow‐up of toxocariasis patients after chemotherapy based on the detection of IgG, IgA, and IgE antibodies by enzyme‐linked immunosorbent assay

Elefant, Guita Rubinsky; Shimizu, Sumie Hoshino; Sanchez, Maria Carmen Arroyo; Jacob, Cristina Miuki Abe; Ferreira, Antônio Walter

doi:10.1002/jcla.20126

Cited by 119 publications

(154 citation statements)

References 32 publications

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“…We also note that the presence of mixed infections between Toxocara and other helminthes might increase the frequency of seropositivity using conventional serological tests, but with the previous serum absorption with Ascaris suum antigens, we might diminish or avoid the possible cross-reactions, as recommended by other authors 6,23 .…”

Section: Discussionmentioning

confidence: 84%

Human toxocariasis: a seroepidemiological survey in the Amazonian city of Yurimaguas, Peru

Roldán

Cavero

Espinoza

et al. 2010

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe aim of this study was to determine the seroprevalence of the infection by Toxocara in the general population of the Amazonian city of Yurimaguas, Peru. From March to August 2008, a total of 300 subjects were sampled and tested by means of a Toxocara ELISA-IgG test. A clinical and epidemiological questionnaire was used to assess the symptomatology and risk factors associated with human toxocariasis. The overall rate of seropositivity was 35.66%, with a significant high proportion in children (p < 0.001). The clinical evaluation revealed that 95.33% of the seropositive group had some type of symptomatology: headache (66.36%), respiratory compromise (63.55%), abdominal pain (54.21%), cutaneous signs (40.19%) and ocular manifestations (36.45%), and almost all of them were statistically significant (p < 0.001). Furthermore, 56.07% of the seropositive subjects presented at least one intestinal pathogen parasite with predominance of helminthes, but without significant association (p = 0.334). The analysis of risk factors showed only that the use of public places and geophagia exhibited a significant association with the seropositivity (p < 0.001). Clinical, serological and epidemiological findings associated to infection with Toxocara were observed in the present study and future studies should be done to assess this serious health problem.

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Section: Discussionmentioning

confidence: 84%

Human toxocariasis: a seroepidemiological survey in the Amazonian city of Yurimaguas, Peru

Roldán

Cavero

Espinoza

et al. 2010

Rev. Inst. Med. trop. S. Paulo

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“…Nevertheless, it is not a good choice to reduce cross-reactions when cases of strongyloidiasis, hydatidosis or fascioliasis are implicated. Perhaps a possible solution may be the use of a more concentrated antigenic extract to avoid the crossreactive antibodies from the sera, although Elefant et al (2006) have used lower concentrations to avoid unspecific antibodies before the ELISA assays.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of an enzyme-linked immunoelectrotransfer blot test for the confirmatory serodiagnosis of human toxocariasis

Roldán

Espinoza

2009

Mem. Inst. Oswaldo Cruz

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To improve the serodiagnosis of human toxocariasis, a sensitive and specific enzyme-linked immunoelectrotransfer blot (EITB-IgG) test was developed and evaluated using Toxocara canis larvae excretory-secretory antigens for detecting anti-Toxocara IgG antibodies. The EITB-IgG profile of toxocariasis was characterized by comparing 27 sera from patients with toxocariasis, 110 sera from healthy subjects and 186 sera from patients with other helminth diseases (ascariasis, ancylostomiasis, trichuriasis, enterobiasis, strongyloidiasis, hymenolepiasis, diphyllobothriasis, taeniasis, cysticercosis, hydatidosis and fascioliasis). Antigenic bands of 24, 28, 30, 35, 56, 117, 136

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“…An indirect ELISA test was performed to evaluate the production of IgG anti-T. canis antibodies according to the protocol described elsewhere (DE SAVIGNY; VOLLER; WOODRUFF, 1979) with slight modifications (ELEFANT et al, 2006).…”

Section: Elisa (Enzyme-linked Immunosorbent Assay)mentioning

confidence: 99%

Detection of larvae of Toxocara canis in milk: an experimental study in rabbits

et al. 2014

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Toxocariasis, caused most commonly by Toxocara canis, is an important cosmopolitan zoonosis. Paratenic hosts have been employed to provide knowledge regard to the transmission of toxocariasis. Transmammary transmission in murine experimentally infected was observed based on the recovery of larvae from the tissue. The aim of this study was to evaluate the possibility of transmammary transmission of Toxocara canis in rabbits by detecting larvae directly in milk. Seventeen sexually mature virgin white New Zealand female rabbits were divided into two groups. Twelve animals were orally inoculated with 1,000 T. canis embryonated eggs (infected group), and five animals remained uninfected (control group). One month following the infection, the females were mated. Manual collection of 500 µL of milk from each rabbit was performed on days +7, +14 and +21 of lactation for three consecutive lactations. The recovery of larvae was determined via a centrifuge-sedimentation technique using ether and formalin solutions. ELISA test was run to confirm the production of anti-T. canis antibodies (IgG) by infected rabbits. The presence of larvae was observed in milk samples from 5 (41.7%) of the 12 infected rabbits. The total number of recovered larvae was 20, ranging from 1 to 4 larvae per lactation/rabbit. Larvae were recovered exclusively on days 7 and 14 of lactation. Recovery was verified in different lactations. No significant difference was observed with respect to the number of larvae either in the same lactation period or in different lactation periods. Anti-T. canis antibodies were detected in all infected rabbits. In conclusion, the presence of larvae in rabbit milk samples suggests the possibility of galactogenic transmission of T. canis in paratenic hosts. Moreover, the technique employed in this study allows for the recovery of larvae directly from milk. a recuperação de larvas. O objetivo do presente estudo foi de avaliar a possibilidade de transmissão transmamária de Toxocara canis em coelhos, pela detecção direta de larvas no leite. Dezessete coelhas (Nova Zelândia branca) púberes e virgens foram distribuídas em dois grupos. Doze fêmeas foram infectadas com 1000 ovos embrionados de T. canis (Grupo Infectado), por via oral, enquanto outras cinco coelhas foram mantidas sem infecção (Grupo Controle). Um mês após a inoculação, as coelhas foram acasaladas. Nos dias +7, +14 e +21 após o nascimento dos filhotes, foram coletados, por ordenha manual, 500µL de leite, em três lactações consecutivas. A recuperação de larvas foi determinada pelo uso da técnica de centrífugo-sedimentação com formol-éter. A técnica de ELISA foi empregada para confirmar a produção de anticorpos (IgG) anti-T. canis pelas fêmeas infectadas. Observou-se a presença de larvas em cinco das doze (41,7%) coelhas por amostra. As larvas foram recuperadas exclusivamente nos dias +7, +14 de lactação. A detecção foi observada em diferentes lactações. Não houve diferença significativa entre o número de larvas na mesma lactação ou entre as diferentes lact...

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A serological follow‐up of toxocariasis patients after chemotherapy based on the detection of IgG, IgA, and IgE antibodies by enzyme‐linked immunosorbent assay

Cited by 119 publications

References 32 publications

Human toxocariasis: a seroepidemiological survey in the Amazonian city of Yurimaguas, Peru

Human toxocariasis: a seroepidemiological survey in the Amazonian city of Yurimaguas, Peru

Evaluation of an enzyme-linked immunoelectrotransfer blot test for the confirmatory serodiagnosis of human toxocariasis

Detection of larvae of Toxocara canis in milk: an experimental study in rabbits

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