A sequential protocol combining dual neuroanatomical tract-tracing with the visualization of local circuit neurons within the striatum

Gonzalo, Nancy; Moreno, Ainhoa; Erdozain, Marı́a A; Garcia, Pedro Braga Linhares; Vázquez, Armando González; Castle, M.; Lanciego, José L.

doi:10.1016/s0165-0270(01)00440-x

Cited by 10 publications

(5 citation statements)

References 55 publications

(54 reference statements)

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“…The latter is usually done via an antibody that highlights a specific type of neuron (presumed GABAergic, peptidergic, cholinergic, or dopaminergic targeted neurons, to name just a few). Initial attempts in this realm included experiments with 'absorption' tags, that is visualization with different light absorption ('colored') chromogens such as diaminobenzidine (brown precipitate), nickel-enhanced diaminobenzidine (black precipitate), tetramethylbenzidine (dark blue precipitate), and Vector VIP™ (magenta precipitate) (Wouterlood et al 1987;Lanciego et al 1997Lanciego et al , 2000Köbbert et al 2000;Gonzalo et al 2001;Lanciego and Wouterlood 2011 (review); Unal et al 2014), while the introduction of confocal laser microscopy, and in parallel the introduction of robust fluorochromes, delivered resolution high enough to reliably detect markers even inside thin fragile fibers and axon varicosities (Wouterlood et al 2007). Most work, nowadays, published in this field is documented with confocal microscopy (e.g., Boulland et al 2009;Barbier et al 2018).…”

Section: Multi-dimensional Tracing With Pha-lmentioning

confidence: 99%

Neuroanatomical tract-tracing techniques that did go viral

Lanciego

Wouterlood

2020

Brain Struct Funct

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Neuroanatomical tracing methods remain fundamental for elucidating the complexity of brain circuits. During the past decades, the technical arsenal at our disposal has been greatly enriched, with a steady supply of fresh arrivals. This paper provides a landscape view of classical and modern tools for tract-tracing purposes. Focus is placed on methods that have gone viral, i.e., became most widespread used and fully reliable. To keep an historical perspective, we start by reviewing one-dimensional, standalone transport-tracing tools; these including today’s two most favorite anterograde neuroanatomical tracers such as Phaseolus vulgaris-leucoagglutinin and biotinylated dextran amine. Next, emphasis is placed on several classical tools widely used for retrograde neuroanatomical tracing purposes, where Fluoro-Gold in our opinion represents the best example. Furthermore, it is worth noting that multi-dimensional paradigms can be designed by combining different tracers or by applying a given tracer together with detecting one or more neurochemical substances, as illustrated here with several examples. Finally, it is without any doubt that we are currently witnessing the unstoppable and spectacular rise of modern molecular-genetic techniques based on the use of modified viruses as delivery vehicles for genetic material, therefore, pushing the tract-tracing field forward into a new era. In summary, here, we aim to provide neuroscientists with the advice and background required when facing a choice on which neuroanatomical tracer—or combination thereof—might be best suited for addressing a given experimental design.

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Section: Multi-dimensional Tracing With Pha-lmentioning

confidence: 99%

Neuroanatomical tract-tracing techniques that did go viral

Lanciego

Wouterlood

2020

Brain Struct Funct

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“…Minor changes have to be made when undergoing interneuron detection. Detailed information dealing with histological processing can be obtained elsewhere Kobbert et al, 2000;Gonzalo et al, 2001).…”

Section: Histologymentioning

confidence: 99%

“…The present report is focused on the analysis of thalamostriatal and thalamo-subthalamic circuits arising from the PF nucleus by using multiple neuroanatomical tracing methods (Gonzalo et al, 2001). Emphasis was placed on the elucidation of the presumptive postsynaptically targeted neurons within both the striatum and STN.…”

Section: Introductionmentioning

confidence: 99%

The parafascicular thalamic complex and basal ganglia circuitry: further complexity to the basal ganglia model

Gonzalo¹,

Lanciego²,

Castle³

et al. 2002

THL

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This work is focused on the study of neuronal circuits arising from the rodent caudal intralaminar nuclei and their presumed role on basal ganglia function. Emphasis was placed on the analysis of the architecture of thalamostriatal and thalamo-subthalamic projections in albino rats. Our major interest was to elucidate whether thalamic inputs were related to projection neurons or local circuit neurons within targeted structures (striatum and subthalamic nucleus). Projections coming from the parafascicular nucleus (PF) to the striatum displayed a patchy organization throughout the matrix compartment. These patches are composed by dense terminal axonal arborizations, often containing striatal neurons projecting to the entopeduncular nucleus (ENT) (medial globus pallidus in primates) and neurons projecting to the substantia nigra reticulata (SNR). The thalamostriatal projections under scrutiny were also seen to be in register with all the major classes of striatal interneurons (nitrergic neurons, neurons containing the calcium binding protein parvalbumin (PV) and cholinergic interneurons). Subthalamic neurons projecting to the ENT are the presumed postsynaptic target for fibers coming from the sensorimotor part (dorsolateral) of the PF. In summary, glutamatergic axons arising from the PF might exert a dual control of the striatal output, either by directly exciting striatal projection neurons or indirectly by means of a previous synaptic contact onto an striatal interneuron which in turn modulates the activity of projection neurons. Furthermore, thalamic inputs can also gain access to basal ganglia output nuclei via subthalamo-pallidal projecting neurons, neurons receiving glutamatergic thalamo-subthalamic projections. Thus, activation of either circuit has an opposite physiological effect on the basal ganglia output nucleus. Taken together, these data suggest that the PF may influence neuronal activity in the direct and indirect circuits and could be considered as an additional component of the basal ganglia motor loops.

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“…Nickel salts have been used at much higher concentrations in DAB–Tris than any other metal (Hancock, 1982, 1984; Tago et al , 1986; Lanciego et al , 1997; Tajima et al , 2000; Gonzalo et al , 2001). To further investigate this phenomenon, dot blots were prepared, as described earlier, from Tris‐buffered DAB solutions containing 1, 5, 20 or 100 mM NiCl 2 and incubated, with or without treatment with 0.3% Na 2 S, for 10 min in developer.…”

mentioning

confidence: 99%

The amplification of polymerized diaminobenzidine with physical developers: sensitizing effects of transition metal salts and sulphide

Ruhland

Jasani

2010

Journal of Microscopy

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SummaryAmplification of metal-complexed polymerized diaminobenzidine by two light-insensitive physical developers was systematically examined in a dot blot model system following either polymerizing diaminobenzidine in the presence of transition metal salts or applying the metal salts post-diaminobenzidine polymerization. The effect of sodium sulphide treatment on subsequent amplification was also investigated. Those metal-diaminobenzidine complexes that facilitated the most powerful amplification were subsequently tested in an immunohistochemical setting. The most dramatic amplification of polymerized diaminobenzidine was observed following its post-polymerization treatment with salts of platinum alone, or gold or vanadium with subsequent sulphide treatment, and allowed previously invisible quantities of polymerized diaminobenzidine to be clearly seen. Three other transition metal salts also improved the amplification of polymerized diaminobenzidine but to a lesser degree, namely nickel alone, and silver or rhodium with subsequent sulphide treatment. Sensitivity was comparable with the colloidal gold-silver amplification system.

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A sequential protocol combining dual neuroanatomical tract-tracing with the visualization of local circuit neurons within the striatum

Cited by 10 publications

References 55 publications

Neuroanatomical tract-tracing techniques that did go viral

Neuroanatomical tract-tracing techniques that did go viral

The parafascicular thalamic complex and basal ganglia circuitry: further complexity to the basal ganglia model

The amplification of polymerized diaminobenzidine with physical developers: sensitizing effects of transition metal salts and sulphide

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