A sequence-specific DNA-binding factor (VF1) from Anabaena sp. strain PCC 7120 vegetative cells binds to three adjacent sites in the xisA upstream region

Chastain, Chris J.; Brusca, John S.; Ramasubramanian, T.; Wei, Tingcun; Golden, James W.

doi:10.1128/jb.172.9.5044-5051.1990

Cited by 53 publications

(31 citation statements)

References 18 publications

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“…Binding and footprinting assays showed that, in vitro, NtcA can bind to sites in both promoters. Similar observations have been made with the NtcA-dependent promoters of nir and xisA (Chastain et al, 1990;Frías et al, 2000). Like other regulators of the CAP family, NtcA appears to act either as an activator or as a repressor depending on the metabolic status of the cells (Kolb et al, 1993;Herrero et al, 2001).…”

Section: Discussionsupporting

confidence: 60%

Expression of furA is modulated by NtcA and strongly enhanced in heterocysts of Anabaena sp. PCC 7120

et al. 2007

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Section: Discussionsupporting

confidence: 60%

Expression of furA is modulated by NtcA and strongly enhanced in heterocysts of Anabaena sp. PCC 7120

et al. 2007

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“…1). The xisA promoter fragment under similar conditions (11). The complex formed with nifH shifted a significantly smaller proportion of the labeled fragment than those with the other probes, indicating a weaker binding affinity under these conditions.…”

mentioning

confidence: 72%

“…Differential gene regulation in vegetative cells and heterocysts is probably caused by different RNA (11). Alternatively, the cells were broken by using a cell disruptor with an air-driven pump (Stansted Fluid Power Limited, Stansted, Essex, England).…”

mentioning

confidence: 99%

Two Anabaena sp. strain PCC 7120 DNA-binding factors interact with vegetative cell- and heterocyst-specific genes

1994

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The DNA-binding factor BifA (previously called VF1) binds upstream of the developmentally regulated site-specific recombinase gene xisA in the cyanobacterium Anabaena sp. strain PCC 7120. Besides binding xisA, BifA also binds the glnA, rbcL, and nifH promoter regions. DNase I footprint analysis of BifA binding to glnA showed a protected region -125 to -148 bp upstream of the translation start site. The binding site is between the major glnA transcription start site used in vegetative cells (RNAII) and the major transcription start site used under nitrogen-deficient conditions (RNAI). The two BifA-binding sites on the rbcL promoter were localized to a 24-bp region from +12 to -12 nucleotides and to a 12-bp region from -43 to -54 nucleotides with respect to the transcription start site. Comparison of the BifA binding sites on the glnA, xisA, and rbcL upstream regions revealed the consensus recognition sequence TGT(N9 or 10) ACA. We have identified a second DNA-binding activity (factor 2) that interacts with rbcL and xisA upstream regions. Factor 2 can be resolved from BifA by heparin-Sepharose chromatography and was present in a bifA mutant. Analysis of partially purified vegetative cell and heterocyst extracts showed that whereas BifA was present in both cell types, factor 2 was present only in vegetative cells. DNase I footprint analysis of factor 2 binding to rbcL showed protection of a 63-bp region between positions -15 and -77 with respect to the transcription start site. The factor 2 binding site on xisA was localized to a 68-bp region that showed considerable overlap with the BifA binding sites.

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“…A homolog of NtcA, which was characterized in Anabaena sp. strain PCC 7120, was found to be synonymous with BifA (VF1), which binds to three adjacent sites in the upstream region of the xisA gene, which encodes a site-specific recombinase involved in the excision of an 11-kb DNA fragment from within nifD (6,57). NtcA is also required for heterocyst differentiation in Anabaena sp.…”

Section: Discussionmentioning

confidence: 99%

GlbN (cyanoglobin) is a peripheral membrane protein that is restricted to certain Nostoc spp

et al. 1996

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The glbN gene of Nostoc commune UTEX 584 is juxtaposed to nifU and nifH, and it encodes a 12-kDa monomeric hemoglobin that binds oxygen with high affinity. In N. commune UTEX 584, maximum accumulation of GlbN occurred in both the heterocysts and vegetative cells of nitrogen-fixing cultures when the rate of oxygen evolution was repressed to less than 25 mol of O 2 mg of chlorophyll a ؊1 h ؊1 . Accumulation of GlbN coincided with maximum synthesis of NifH and ferredoxin NADP ؉ oxidoreductase (PetH or FNR). A total of 41 strains of cyanobacteria, including 40 nitrogen fixers and representing 16 genera within all five sections of the cyanobacteria were screened for the presence of glbN or GlbN. glbN was present in five Nostoc strains in a single copy. Genomic DNAs from 11 other Nostoc and Anabaena strains, including Anabaena sp. strain PCC 7120, provided no hybridization signals with a glbN probe. A constitutively expressed, 18-kDa protein which cross-reacted strongly with GlbN antibodies was detected in four Anabaena and Nostoc strains and in Trichodesmium thiebautii. The nifU-nifH intergenic region of Nostoc sp. strain MUN 8820 was sequenced (1,229 bp) and was approximately 95% identical to the equivalent region in N. commune UTEX 584. Each strand of the DNA from the nifU-nifH intergenic regions of both strains has the potential to fold into secondary structures in which more than 50% of the bases are internally paired. Mobility shift assays confirmed that NtcA (BifA) bound a site in the nifU-glbN intergenic region of N. commune UTEX 584 approximately 100 bases upstream from the translation initiation site of glbN. This site showed extensive sequence similarity with the promoter region of glnA from Synechococcus sp. strain PCC 7942. In vivo, GlbN had a specific and prominent subcellular location around the periphery of the cytosolic face of the cell membrane, and the protein was found solely in the soluble fraction of cell extracts. Our hypothesis is that GlbN scavenges oxygen for and is a component of a membrane-associated microaerobically induced terminal cytochrome oxidase.

show abstract

A sequence-specific DNA-binding factor (VF1) from Anabaena sp. strain PCC 7120 vegetative cells binds to three adjacent sites in the xisA upstream region

Cited by 53 publications

References 18 publications

Expression of furA is modulated by NtcA and strongly enhanced in heterocysts of Anabaena sp. PCC 7120

Expression of furA is modulated by NtcA and strongly enhanced in heterocysts of Anabaena sp. PCC 7120

Two Anabaena sp. strain PCC 7120 DNA-binding factors interact with vegetative cell- and heterocyst-specific genes

GlbN (cyanoglobin) is a peripheral membrane protein that is restricted to certain Nostoc spp

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