A Sequence-Ready Physical Map of a Region of 12q24.1

Renault, Béatrice; Hovnanian, Alain; Bryce, Steven D.; Chang, Joan-Jung; Lau, Stephanie; Sakuntabhai, Anavaj; Monk, Sarah; Carter, Simon; Ross, Colin J.D.; Pang, J T; Twells, Rebecca C.J.; Chamberlain, Susan; Monaco, Anthony P.; Strachan, Tom; Kucherlapati, Raju

doi:10.1006/geno.1997.4888

Cited by 9 publications

(10 citation statements)

References 34 publications

(1 reference statement)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…YAC 678b5, which contains the three OAS genes, was not part of the previous maps of the region. In our study, YAC 910g1 was found to be negative for markers distal to OAS1 (p40/p46), whereas it was reported to be positive for D12S1895, D12S1888, and D12S2293 (Renault et al, 1997). We also mapped two new highly polymorphic microsatellite markers isolated from PAC223G16 (D12S2396) and the 3Ј UTR of OAS3 (p100) (D12S2397) (Monk et al, 1998), which were not present on the existing contigs.…”

Section: Discussioncontrasting

confidence: 58%

“…The OAS locus is at the telomeric part of two larger contigs of chromosomal region 12q24.1 that were recently reported (Nechiporuck et al, 1997;Renault et al, 1997). Both contigs show that OAS-40/46, termed OIASI for "oligoisoadenylate synthetase" (Renault et al, 1997) and IFI-4 for "interferon induced" (Nechiporuck et al, 1997), maps to a similar position on 12q24.2.…”

Section: Discussionmentioning

confidence: 91%

“…Both contigs show that OAS-40/46, termed OIASI for "oligoisoadenylate synthetase" (Renault et al, 1997) and IFI-4 for "interferon induced" (Nechiporuck et al, 1997), maps to a similar position on 12q24.2. The markers D12S1895 and D12S1888 are mapped telomeric to OAS-40/46 (Renault et al, 1997), but these ESTs were not identified as deriving from OAS-69/71. Mapping of a third OAS gene between the OAS-40/46 and OAS-69/71 genes provides new information on the organization of the three OAS genes and improves the accuracy of the map of this region of chromosome 12.…”

Section: Discussionmentioning

confidence: 95%

“…On the basis of these hybridization results, we concluded that PACs 57I11 and 336L20 carried the three genes for OAS-40/46, -100, and -69/71 with no evidence for . The relative order of WI-11137, D12S2300, and WI-10614 refers to the map by Renault et al (1997). The cosmid contig (see Table 2) is represented by a gray horizontal bar.…”

Section: Analysis Of Pac Clones Reveals Clustering Of the Three Oas Gmentioning

confidence: 99%

See 3 more Smart Citations

The Human 2′,5′-Oligoadenylate Synthetase Locus Is Composed of Three Distinct Genes Clustered on Chromosome 12q24.2 Encoding the 100-, 69-, and 40-kDa Forms

et al. 1998

Self Cite

View full text Add to dashboard Cite

Section: Discussioncontrasting

confidence: 58%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 95%

Section: Analysis Of Pac Clones Reveals Clustering Of the Three Oas Gmentioning

confidence: 99%

See 2 more Smart Citations

The Human 2′,5′-Oligoadenylate Synthetase Locus Is Composed of Three Distinct Genes Clustered on Chromosome 12q24.2 Encoding the 100-, 69-, and 40-kDa Forms

et al. 1998

Self Cite

View full text Add to dashboard Cite

“…If a rearrangement occurred during the bacterial transformation or shortly thereafter, then independently obtained overlapping clones would end up with inconsistent genomic structures. Therefore, the 169 overlapping BACs from the 1.5-Mb contig were compared by pulsed-field gel electrophoresis, high-resolution STS-content mapping (Renault et al 1997), and fingerprinting (Marra et al 1997). Five parallel subcolonies from each BAC were analyzed by pulsed-field gel electrophoresis to exclude the occurrence of major rearrangements or clonal con-tamination.…”

Section: Clone Rearrangementsmentioning

confidence: 99%

A Bacterial Artificial Chromosome Library for Sequencing the Complete Human Genome

Osoegawa¹,

Mammoser²,

Wu³

et al. 2001

Genome Res.

290

233

View full text Add to dashboard Cite

A 30-fold redundant human bacterial artificial chromosome (BAC) library with a large average insert size (178 kb) has been constructed to provide the intermediate substrate for the international genome sequencing effort. The DNA was obtained from a single anonymous volunteer, whose identity was protected through a double-blind donor selection protocol. DNA fragments were generated by partial digestion with EcoRI (library segments 1–4: 24-fold) and MboI (segment 5: sixfold) and cloned into the pBACe3.6 and pTARBAC1 vectors, respectively. The quality of the library was assessed by extensive analysis of 169 clones for rearrangements and artifacts. Eighteen BACs (11%) revealed minor insert rearrangements, and none was chimeric. This BAC library, designated as “RPCI-11,” has been used widely as the central resource for insert-end sequencing, clone fingerprinting, high-throughput sequence analysis and as a source of mapped clones for diagnostic and functional studies.The sequence data described in this paper have been submitted to the GenBank data library under accession nos. AQ936150–AQ936491.]

show abstract

Genome Physical Mapping UsingBACs

Altmann¹

2000

Encyclopedia of Analytical Chemistry

View full text Add to dashboard Cite

A physical map of a region or the entirety of the genome of an organism is a representation of the genome providing information on the relative order and the arrangement of physically defined entities. For most physical maps, these entities are cloned genomic DNA fragments propagated in cosmids, bacteriophage P1, bacterial artificial chromosomes (BACs), P1‐derived artificial chromosomes (PACs), or yeast artificial chromosomes (YACs). The various cloning systems differ in the sizes of the cloned DNA fragments and the fidelity of DNA structure maintenance. Owing to its excellent properties, the BAC system is the premier system for this purpose. Several different, highly efficient procedures have been developed and applied successfully to identify overlaps between the DNA fragments represented in different clones. The procedures are based on different experimental techniques, including nucleic acid hybridization, DNA amplification through polymerase chain reaction (PCR), DNA restriction fragment analysis, and DNA sequencing. Applied separately or in combination, these procedures have been used successfully to generate extensive physical maps of human, animal, and plant genomes.

show abstract

A Sequence-Ready Physical Map of a Region of 12q24.1

Cited by 9 publications

References 34 publications

The Human 2′,5′-Oligoadenylate Synthetase Locus Is Composed of Three Distinct Genes Clustered on Chromosome 12q24.2 Encoding the 100-, 69-, and 40-kDa Forms

The Human 2′,5′-Oligoadenylate Synthetase Locus Is Composed of Three Distinct Genes Clustered on Chromosome 12q24.2 Encoding the 100-, 69-, and 40-kDa Forms

A Bacterial Artificial Chromosome Library for Sequencing the Complete Human Genome

Genome Physical Mapping UsingBACs

Contact Info

Product

Resources

About